Plants have evolved elaborate innate immune systems against invading pathogens, such as bacteria, fungi, oomycetes, viruses and insects. Among them, intracellular immune receptors known as nucleotide-binding site and leucine-rich repeat (NB-LRR) play critical roles in effector-triggered immunity (ETI) regarding to plant defense. Here, we identified potential NB-LRR coding sequences from pepper genome using bioinformatics analysis and performed comparative analysis with Solanaceae plants. As a result, we identified 267, 443, and 755 NBS-encoding genes in the genome of tomato, potato, and pepper, respectively. These may indicate that the Solanaceae NB-LRRs were evolved through species-specific unequal-duplication event. Further phylogenetic and clustering analyses revealed that Solanaceae NB-LRRs were classified into the 14 subgroups with 1 TNL and 13 CNL types. We found that the genes in CNL-G1 and CNL-G2 subgroup were highly expanded compared to other subgroup showing a large portion of NB-LRR in pepper genome. Among 755 NB-LRRs in pepper genome, 623 were physically mapped on all 12 pepper chromosome pseudomolecules. Furthermore, a number of NB-LRRs in the same group were physically clustered by tandem array in the specific chromosome. Genome-wide identification of pepper NB-LRR family and their evolutionary analysis could provide an important resource for identification and characterization of genes for breeding of disease resistance crops.

Adipogenesis is critical in development and homeostasis of energy metabolism. However, in these days, the obesity has become prevalent and became a cause of medial complication. Various applications have been suggested to prevent or decrease accumulaiton of energy in fat cells. However, those have little usefulness and have various side effects. Diphlorethohydroxy-carmalol (DPHC) is a phlorotannin compound, with various biological activities in vitro and in vivo. In here, we studied that DPHC could modify the accumulation of fat on integument. The size of adipocytes and thickness of the subcutanous fat tissue was analyzed after treatment of cosmetics contained 0, 0.01, 0.1, 1, or 10 % DPHC using NIS Element D 4.10.00 software (Nikon). The viability and proliferation of cell was analyzed after 0, 0.4, 2, 10, or 50μg/ml of DPHC treatment using MTT (3-[4,5-dimethylthiazo-2-yl]-2,5-diphenyl tetrazolium bromide) assay (R&D system, Cat # 48090-025-k) and measurment of doubling time. Accumulation of lipids in differentiating preadipocytes was analyzed with spectrophotometer after Oil Red-O staining. The size of adipocyte and thickness in skin was decreased in DPHC treated mice. The metabolic activity and doubling of 3T3-L1 were suppressed by DPHC in concentration dependent manner. DPHC also inhibit accumulation of lipids in the adipocyte. The expression of the marker genes for adipocyte differentiation coincided with cytochemical results. Base on them, it is suggested that DPHC has antiobesity effects in integument through suppress accumulation of lipids and suppress the proliferation and differentiation both of adipose stem cells and precursor cells.

Chili pepper (Capsicum annuum) is one of the most important vegetable crop for human being as a rich source of nutritions and spicy condiments. To make reference genome sequence of pepper, we sequenced the whole genome of Capsicum annuum, CM334 using Illumina/Solexa Genome Analyzer GA2. The genome size of CM334 is estimated as 3.5 Gb. A total of 716 Gb (205.96x coverages of the whole genome) of raw sequences were generated. After filtering out the low quality sequences, a total of 233 Gb (66.7x) of the raw sequences were used for assembly. Total assembled contig length and number were 2.93 Gb and 295,502, respectively. N50 and average length were 25.72 kb and 6,5 kb, respectively. By sequencial scaffolding with mate-pair sequences of 2 kb - 20 kb sizes, a total of 3.04 Gb of scaffold which is approximately 90% of the whole genome was assembled. The total number of scaffolds was 33,876 with N50 length of 1,605 kb. For annotation of the pepper genome, a total of 46 Gb of transcriptome sequences were generated from 12 different tissues using Illumina GA2 and Hi-seq 2000. We are under way of analyzing the characteristic traits of pepper using transcriptome data. The progress of pepper genome sequencing project including gene annotation, gene family analysis, comparative genomics studies on evolution of hot taste, genome expansion and fruit development will be presented in the meeting.

In this paper, we designed and fabricated the Electromagnetic(EM) wave absorber for an Electronic Toll Collection(ETC or Hi-pass) system by using Amorphous metal powder and CPE. The material properties and the absorption properties of the samples containing 50 wt.%, 60 wt.%, 70 wt.%, and 80 wt.% of Amorphous. Moreover, the EM wave absorption abilities were simulated for the different thicknesses of the EM absorbers by adopting the measured permittivity and permeability, and then the EM wave absorber was fabricated based on the simulated design values. As a result, the EM wave absorber with the composition of Amorphous metal powder : CPE = 50 : 50 wt.% with the thickness of 2.6 mm has excellent absorption ability more than 40 dB at 5.8 GHz.

In this paper, we designed and fabricated the Electro-Magnetic (EM) wave absorber for wireless LAN by using Amorphous and CPE. The material constants and the absorption properties were measured for the samples containing 50 %, 60 %, and 70 % weight fraction of Amorphous. Moreover, the EM wave absorption abilities were simulated for the EM absorbers in different thicknesses by adopting the measured permittivity and permeability, and then the EM wave absorber was fabricated based on the simulated design values. As a result, the EM wave absorber with the composition ratio in Amorphous : CPE = 60 : 40 wt.% with the thickness of 4 mm has the absorption ability more than 35 dB at 2.4 GHz. Thus, it is expected the wireless LAN environment can be improved by using the developed absorber.

In Korea, chilli pepper (Capsicum annum L.) is a major vegetable crop. The pepper seed market is about $35 million and the whole sale market including processed products is equivalent to $2 billion, representing the second highest market value among crops, next to rice in Korea. Since the development of elite pepper variety is so competitive, vegetable seed companies usually run two important programs to keep the credibility of seed quality. One program is to deliver F1 hybrid seeds with a high purity test to farmers. The purity control of parents and F1 hybrid to avoid any contamination is conducted by DNA markers because pepper seeds are obtained using MS line. The other program is to identify the F1 variety from other varieties by analyzing the polymorphism so that the company and/or breeder protects the intellectual property from copying by others or from non-intentional contamination. We have developed about 900 EST-SSR sets from pepper and used to both programs. A total of 66 markers were selected to identify 32 F1 varieties and their own parents. Using these markers, the purity control of F1 hybrid rose up to the highest degree. We also found several SSR markers to distinguish F1 variety from other varieties and these markers could be useful to find the uniqueness of F1 cultivar.

Hopyeongbyeo is an anther culture derived Japonica rice (Oriza sativa L.) cultivar from the cross between Hitomebore and Hwajinbyeo by the rice breeding team of Honam Agricultural Research Institute (HARI), NICS, RDA, in 2003. This cultivar has a about 126 days growth duration from transplanting to harvesting. The short and round grain shape of Hopyeongbyeo is translucent with non-glutinous endosperm. Amylose content of rice grain is about 17.8% and high palatability of cooked rice compared with Nampyeongbyeo. This cultivar shows resistant to bacterial blight pathogenes of K1. The milled rice yield of Hopyeongbyeo is about 5.13MT/ha at ordinary transplanting culture in the local adaptability test. Hopyeongbyeo would be adaptable for the southern plain area of Korea.

In this paper, the EM wave absorbers were designed and fabricated for X -band sensors using Carbon of dielectric material with CPE. The complex relative permittivity of samples is calculated by using measurement results of S-parameter. We simulated the double-layered type EM wave absorber with broad bandwidth using the measured complex relative permittivity by changing the thickness and layer, which was fabricated based on the simulated design The fabricated EM wave absorber consists of 1 mm first layer sheet facing metal with Carbon composition ratio 70 vol. % and 1.5 mm second layer sheet with Carbon composition ratio 60 vol. %. The measured results showed a good agreement to the simulated ones. It is found toot the optimized absorption ability of double-layered type EM wave absorber with thickness of 2.5 mm is higher than 10 dB from 7.8 GHz to 13.3 GHz.

A new compact microstrip low-pass filter and its equivalent-circuit model are developed. The philosophy of the structure behind this novel microstrip low-pass filter is simple as it is composed of a pair of symmetrical parallel coupled-line and an open-stub. With this configuration, a finite attenuation pole near the stopband cutoff frequency is available and adjustable by simply tuning the circuit parameters. Furthermore, the rejection bandwidth of this type of low-pass filter can be extended. In order to validate the feasibility of the proposed design method, a low-pass filter based on a microstrip structure is designed, fabricated, and measured. Experimental results agree very well with the simulation and analytical results.