The wheat-rye translocation lines have been agriculturally developed for the resistance to the biotypes of Hessian fly as a major insect pest of wheat. In order to compare the proteomic profiles between ‘Coker797’ (non-2RL), ‘Hamlet’ (2RL), and near-isogenic line (NIL) carrying 2RL, we evaluated the protein extraction and preparation methods for two-dimensional gel electrophoresis approach. The tissues such as leaves, stems, and roots from three wheat-rye lines were extracted by following trichloroacetic acid (TCA)/acetone precipitation. In a preliminary proteome analysis, a commonly expressed protein in Hamlet and NIL strain was identified as methionine synthase annotated in Hordeum vulgare subsp. The present study will provide the experimental guideline for the proteomic study of other useful crop plant tissues.

A new red leaf lettuce (Lactuca sativa L.) cultivar, “Jangsu” which has late bolting and good red expression leaf color was developed from a cross of “Yeolpungcheokchima” (late bolting and high yield) and “Seoulyakchima” (dark red color). The selection and breeding of advanced lines were done by pedigree method during 2000-2004. The advanced lines were evaluated for yield and adaptability in Korea (Gangwon, Kyunggi, Chungbuk, Kyungnam, Jeonnam, Jeonbuk, and Jeju) from 2005 to 2006. The “Jangsu” has gray seed color and elliptic thick leaves. Compared to “Yeolpungcheokchima”, marketable yield of “Jangsu” was lower by 10% (at 469 g per plant), but with particularly improved good expression of red leaf color in high temperature cultivation in the field. The shelf-life of “Jangsu” was three weeks longer than “Yeolpungcheokchima” at 4℃. The amount of bitter sesquiterpene lactones (BSL) and sugar content of “Jangsu” were similar with “Yeolpungcheokchima” at 46 ㎍/g and 4.8 brix, respectively, but taste is better, more crispy, and sweeter than “Yeolpungcheokchima”.