Toll-like receptor 4 (TLR4) is known to contribute to the modulation of insulin resistance and systemic inflammation seen in obesity and the metabolic syndrome. The present study was performed to investigate the fertility competence of TLR4 knock out male mice (TLR4 mice) on a high-fat diet (HFD), compared to a normal-chow diet (NCD). The controls included wildtype (WT) mice fed on a HFD or NCD. Six-week-old male mice were fed with either a NCD or HFD for 20 weeks. Body and organ weights, serum levels of glucose, triglycerides and hepatoxicity, sperm quality and spermatogenesis were observed after the sacrifice. Also, randomly selected male mice were mated with virgin female mice after feeding of 19 weeks. The weight of the body and organs increased in WT and TLR4 mice on a HFD compared to those of mice on a NCD. The weights of the reproductive organs did not vary among the treatment groups. The motility and concentration of the epididymal spermatozoa decreased in both WT and TLR4 mice fed a HFD. The pregnancy rate and litter size declined in the HFD-fed WT mice compared to the HFD-fed TLR4 mice. In conclusion, the HFD alters energy and steroid metabolism in mice, which may lead to male reproductive disorders. However, fertility competence was somewhat restored in HFD-fed TLR4 male mice, suggesting that the TLR4 is involved in testis dysfunction due to metabolic imbalance.

For the final disposal of radioactive waste, concentration of gamma nuclides such as Co-58, Co-60, Cs-137, Nb-94 have to be determined to meet nuclear regulatory requirements. In general, gamma nuclide analysis can be performed with simple sample pretreatment without complicated chemical separation processes due to the characteristics of the nuclide and high resolution of the measuring equipment. However, when the concentration of Co-60 is high in a specific radioactive waste generated at the NPP, the background is increased by the compton continuum of Co-60. That makes it difficult to evaluate accurately Nb-94, which is in the lower energy band than the gamma ray energy region of Co-60 and especially Cs-137, which is used as a key nuclide of scaling factor. In this study, We consider the problem of MDA dissatisfaction or overestimation due to the increased background by Co-60.

Many radionuclides emit two or more gamma rays in a cascade once they decay. At this time, gamma rays are detected at the same time, and the signals are overlapped and measured as one added signal. This is called the summing coincidence effect, and it causes an error of more than 10% depending on the detection efficiency, measurement conditions, and target nuclide. It is known to be greater as the efficiency of the detector increases and as the distance between the source and the detector decreases. It is necessary to consider the summing coincidence effect since the efficiency of the HPGe detector owned by the KHNP CRI is as high as 65%. In this study, We would like to propose an appropriate gamma nuclide analysis method for radioactive waste generated from NPP by evaluating the influence on the summing coincidence effect.

As nuclear power plants are operated in Korea, low and intermediate-level radioactive wastes and spent nuclear fuels are continuously generated. Due to the increase in the amount of radioactive waste generated, the demand for transportation of radioactive wastes in Korea is increasing. This can have radiological effect for public and worker, risk assessment for radioactive waste transportation should be preceded. Especially, if the radionuclides release in the ocean because of ship sinking accident, it can cause internal exposure by ingestion of aquatic foods. Thus, it is necessary to analyze process of internal exposure due to ingestion. The object of this study is to analyze internal exposure by ingestion of aquatic foods. In this study, we analyzed the process and the evaluation methodology of internal exposure caused by aquatic foods ingestion in MARINRAD, a risk assessment code for marine transport sinking accidents developed by the Sandia National Laboratory (SNL). To calculate the ingestion internal exposure dose, the ingestion concentrations of radionuclides caused by the food chain are calculated first. For this purpose, MARINRAD divide the food chain into three stages; prey, primary predator, and secondary predator. Marine species in each food chain are not specific but general to accommodate a wide variety of global consumer groups. The ingestion concentrations of radionuclides are expressed as an ingestion concentration factors. In the case of prey, the ingestion concentration factors apply the value derived from biological experiments. The predator's ingestion concentration factors are calculated by considering factors such as fraction of nuclide absorbed in gut, ingestion rate, etc. When calculating the ingestion internal exposure dose, the previously calculated ingestion concentration factor, consumption of aquatic food, and dose conversion factor for ingestion are considered. MARINRAD assume that humans consume all marine species presented in the food chain. Marine species consumption is assumed approximate and conservative values for generality. In the internal exposure evaluation by aquatic foods ingestion in this study, the ingestion concetration factor considering the food chain, the fraction of nuclide absorbed in predator’s gut, ingestion rate of predator, etc. were considered as influencing factors. In order to evaluate the risk of maritime transportation reflecting domestic characteristics, factors such as domestic food chains and ingestion rate should be considered. The result of this study can be used as basis for risk assessment for maritime transportation in Korea.

After Fukushima nuclear power plant accident in 2011, Concerns about accident of spent fuel pool increase. In Korea, the time of saturation of spent fuel pool is coming, but regulatory measures and safety evaluation are insufficient when occurring spent fuel pool accident. Thus, it is necessary to review of spent fuel pool accident in foreign countries to establish regulatory measures and safety evaluation of spent fuel pool accident suitable for domestic spent fuel pool. Therefore, we reviewed spent fuel pool accident that occurred at Fukushima Unit 4, SONGS Unit 2 and PAKS. In Japan, spent fuel pool accident occurred at Fukushima NPP in 2011. Tsunami was cause of the accident. Station Black Out occurred at Fukushima NPP and Emergency Diesel Generator lost their functions due to Tsunami. As a result, Loss of cooling happened in spent fuel pool at Fukushima NPP. For Unit 4, wall of spent fuel pool in Unit 4 was damaged due to hydrogen explosive, so loss of coolant in spent fuel pool of Unit 4 occurred. After the accident, the temperature of spent fuel pool increases to 75°C, but there was no damage to the spent fuel. In USA, spent fuel pool accident occurred at SONGS Unit 2 in 2013. The debris of nearby ocean is cause of the accident. The debris entered the system through a damaged Salt Water Cooling pump suction strainer. The debris obstructed flow through the Component Cooling Water heat exchanger and operation of Salt Water Cooling. The maximum spent fuel pool temperature during this event was 25.6°C. It was a value that satisfied the technical specifications of the SONGS NPP. In Ukraine, spent fuel pool accident occurred at PAKS in 2003. Unintentionally opened valve of cleaning tank is cause of the accident. Loss of coolant occurred in spent fuel pool of PAKS. Due to loss of coolant, spent fuels were exposed to the vapor state atmosphere, and oxidation occurred in the cladding tube of the spent fuel that rose to 1,400°C. In this study, Review of spent fuel pool accident in major foreign countries was conducted as basic studies for establishing regulatory measures and safety evaluation of spent fuel pool in Korea. Causes of each accident were different by structure of spent fuel pools. Result of this study will be contributed to establish safety measures of spent fuel pool accident suitable for domestic spent fuel pool facility.

Tuberculosis is a potentially deadly infectious disease caused by the Mycobacterium tuberculosis (M. tuberculosis). Tuberculosis is diagnosed by proving the M. tuberculosis in sputum samples based on the results of acid-resistant staining, culture, and nucleic acid amplification tests. However, there is a report that the detection rate of M. tuberculosis is low in acid-resistant staining using tissue specimens. It has been suspected that the cause is a potential loss of acid resistance by the organic solvents used for tissue specimen preparation. Therefore, this study was pursued to find out if Gram staining and fluorescent staining in addition to acid-resistant staining would be helpful in diagnosing tuberculosis. We used four tissue (lung, small intestine, large intestine, and lymph node) samples with chronic granulomatous inflammation observed in HE staining and positive results in real-time PCR. These detection rates and staining properties were investigated through microscopic examination using the Ziehl-Neelsen, Gram, and Auramin rhodamine staining. In this studies, M. tuberculosis were observed by Ziehl-Neelsen, Gram, and Auramin rhodamine staining in all four samples. In the evaluation of clinical microbiology proficiency testing (CMPT), the Ziehl-Neelsen and Gram staining were the same result, but the Auramin rhodamine staining was relatively low. These data indicated that Gram staining is useful for detecting M. tuberculosis in formalin-fixed tissue specimens. Therefore, if the Ziehl-Neelsen and Gram staining are combined as the M. tuberculosis staining method in tissue specimens, a better direction may be provided for tuberculosis diagnosis.