The Stockholm Convention was adopted in Sweden in 2001 to protect human health and the environment, including Persistent Organic Pollutants Rotors, such as toxic and bioaccumulative. Currently, there are 28 kinds of materials. This prohibits and limits the production, use, and manufacture of the product. Korea is a party to the Convention and it is necessary to prepare management and treatment plan to cope with POPs trends. In the text, we have discussed HCBD materials. HCBD belongs to halogenated aliphatic unsaturated hydrocarbons. It is a toxic, organic mixture of bioaccumulation. A study on the treatment of waste containing HCBD substance, We decided to treat the waste containing HCBD thermally. So six samples were selected. Waste water treatment sludge, rubber plate, insecticide, tarpaulin, tire rubber, mixed sample. The tire rubber injected HCBD as a technical sample. HCBD analysis showed that 59.345 ~ 18,238.355 ug/kg was detected. For the thermal treatment, we analyzed element. As a result of thermogravimetric analysis, the weight change due to the decomposition of the material started at 200℃. The material decomposition was completed within 800℃. The thermal treatment was performed on a Lab-scale (1kg/hr). After exhaust gas analysis result, HCBD was detected at 0.01 to 0.09 ug/kg. The decomposition rate is estimated to be 99.848 ~ 99.999%. As a result of dioxin analysis in the exhaust gas, the highest concentration was found in the tarpaulins and the emission limit was exceeded. The concentrations of Cd, Pb, Cr, Cu, Ni and Zn in the residues were very low. Considering the decomposition rate of HCBD containing wastes, incineration treatment at 2 ton/hr or more is considered to be possible. And unintentional persistent organic pollutants such as dioxins in the exhaust gas. Therefore, it is considered safe to operate the incineration temperature at more than 1100℃.

To achieve energy efficiency improvement is used to lower temperature for emission gas at catalyst inlet, or to reduce/stop using steam to reheat emission gas. Saved energy from this process can be used as power source in order to increase generation efficiency. Dry emission gas treatment, on the other hand, is the technology to increase generation efficiency by using highly efficient desalination materials including highly-responsive slaked lime and sodium type chemicals in order to comply with air pollution standards and reduce used steam volume for reheating emission gas. If dry emission gas is available, reheating is possible only with the temperature of 45℃ in order to expect generation efficiency by reducing steam volume for reheating. Retention energy of emission gas from combustion is calculated by emission gas multiplied by specific heat and temperature. In order to obtain more heat recovery from combustion emission gas, it is necessary to reduce not only exothermic loss from boiler facilities but emission calorie of emission gas coming out of boiler facilities. In order to reduce emission calorie of emission gas, it is efficient to realize temperature lowering for the emission gas temperature from the exit of heat recovery facility and reduce emission gas volume. When applying low temperature catalysts, the energy saving features from 0.03% to 2.52% (average 1.28%). When increasing the excess air ratio to 2.0, generation efficiency decreases by 0.41%. When the inlet temperature of the catalyst bed was changed from 210℃ to 180℃, greenhouse gas reduction results were 47.4, 94.8, 118.5, 142.2 thousand tons-CO2/y, CH4 was calculated to be 550.0, 1100.1, 1375.1, 1650.1 kg-CH4/y, and N2O was 275.0, 550.0, 687.6, 825.1 kg-N2O/y. In the case of high efficiency dry flue gas treatment, reduction of greenhouse gases by the change of temperature 120~160℃ and exhaust gas 5,000 ~ 6,500 ㎥/ton is possible with a minimum of 355,461 ton/y of CO2 and minimum 4,125 tons of CH4/y to a maximum of 6,325 ton/y and N2O to a minimum of 2,045 kg/y to a maximum of 3,135 kg/y.

Soluble-NSF attachment protein receptor (SNARE) proteins play a role in vesicle fusion, exocytosis, and intracellular trafficking in neuronal cells as well as in fertilization and embryogenesis. We investigated the expression patterns of two SNARE proteins, SNAP-25 and synaptotagmin VII (SytVII), and their regulation by pregnant mare serum gonadotropin (PMSG) during mouse ovarian follicular development. Ovaries were obtained at 0, 12, 24, 36, and 48 h post-PMSG injection of immature mice. SNAP-25 and SytVII mRNA expression levels increased gradually in a time-dependant manner. However, protein levels revealed different patterns of expression, suggesting different translational regulation following PMSG stimulation. SNAP-25 and SytVII expression was closely associated with thickening of the granulosa cell (GC) layer and follicle morphological changes from a flattened to a cuboidal shape. To explore follicle stimulating hormone receptor (FSHR)-mediated regulation of their expression, GCs from preantral follicles were cultured to examine the effects of FSHR siRNA knockdown. FSHR siRNA abolished upregulation of the SNAREs in both PMSG and FSH-stimulated GCs. This abolished gene expression was rescued by adding dibutyryl cyclic AMP to the cultures. These results suggest that SNAP-25 and SytVII expression is regulated via the FSHR-cAMP pathway during follicular development.

Construction of the Asian Network for Sustainable Organic Farming Technology (ANSOFT) will be cooperatively administered by the public researchers in 12 Asian member countries from 2010 to 2012. ANSOFT will bring forward multiple reports, which will be constantly renewed by the member countries, regarding environmental issues, plant and landscape protection techniques, regulations and policies of each country’s government on an organic ag끼culture， and natural resources such as organic seeds and biological agents.

The occurrence of major pest infestation was compared between conventional tea plantation and organic tea plantation at Sulloc tea garden in Dosun-dong, Seogwipo-si, Jeju-do from 2002 to 2009. Tetranychus kanzawai was observed a lot in the second year, but it waned from late June. There was not much difference between conventional farming and organic farming in terms of pest density. Empoasca onukii was infested in the second year of organic farming compared with conventional farming, which highlighted the fact that second year of organic farming requires a special care. Scirotothrips dorsalis was highly dense in the second and third year of conventional farming, but its occurrence was lowered when the farming technique was shifted to organic farming. The number of Homona magnanima peaked 4 times each year. In 2008, the first year of organic farming, saw high occurrence of 771.2 per trap per year. In 2009, the second year, the population per trap dropped to 80, showing a great variance depending on year. The occurrence of Caloptilia theivora peaked 5 times annually. In 2008, the first year of organic farming, an average of 2,779 pests per trap was found, and in the following year, 4,143 pests were observed. It showed that density rose in organic growing period.

Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.

"Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. DNA methylation is a highly plastic and critical component of mammalian development The DNA methyltransferases (Dnmts) are responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. The maintenance DNA methyltransferase enzyme, Dnmt 1, and the de novo methyltransferase, Dnmt3a and Dnmt3b, are indispensable for development because mice homozygous for the targeted disruption of any of these genes are not viable. The occurrence of DNA methylation is not random, and it can result in gene silencing The mechanisms underlying these processes are poorly understood. It is well established that DNA methylation and histone deacetylation operate along a common mechanistic pathway to repress transcription through the action of methyl-binding domain proteins (MBDs), which are components of, or recruit, histone deacetylase (HDAC) complexes to methylated DNA. As a basis for future studies on the role of the DNA-methyl-transferase in porcine development, we have isolated and characterized a partial cDNA coding for the porcine Dnmt1. Total RNA of testis, lung and ovary was isolated with TRlzol according to the manufacture's specifications. 5 ug of total RNA was reverse transcribed with Super Script II in the presence of porcine Dnmt 1 specific primers. Standard PCRs were performed in a total volume of 50 ul with cDNA as template. Two DNA fragmenets in different position were produced about 700bp, 1500bp and were cloned into pCR II-TOPO according to the manufacture's specification. Assembly of all sequences resulted in a cDNA from 158bp of 5'to 4861bp of 3'compare with the known human maintenance methyltransferase. Now, we are cloning the unknown Dnmt 1 region by 5'-RACE method and expression of Dnmt 1 in tissues from adult porcine animals.