Although nuclear transfer (NT) techniques are used to clone animals, its efficiency is very low. Moreover, nuclear transfer has resulted in offspring with severe developmental problems, probably due to incomplete nuclear reprogramming. Nuclear reprogramming is characterized by functional modification of the transferred nucleus to allow it to direct normal embryo development with the potential to grow to term. Although the nature of the reprogramming factor(s) in mammals is not clear, various nuclear as well as cytoplasmic components are involved in the processes. In this article we review recent data on factors involved in the nuclear reprogramming of cloned embryos.

We present the results of an rocket-borne observation of far-infrared [CII] line at 157.7 μm from the diffuse inter-stellar medium in the Ursa Major. We also introduce a part of results on the [CII] emission recently obtained by the IRTS, a liquid-helium cooled 15cm telescope onboard the Space Flyer Unit. From the rocket-borne observation we obtained the cooling rate of the diffuse HI gas due to the [CII] line emission, which is 1.3±0.2 × 10-26 ergss-1 H-1atom. We also observed appreciable [CII] emission from the molecular clouds, with average CII/CO intensity ratio of 420. The IRTS observation provided the [CII] line emission distribution over large area of the sky along great circles crossing the Galactic plane at I = 50° and I = 230°. We found two components in their intensity distributions, one concentrates on the Galactic plane and the another extends over at least 20° in Galactic latitude. We ascribe one component to the emission from the Galactic disk, and the another one to the emission from the local interstellar gas. The [CII] cooling rate of the latter component is 5.6 ± 2.2 ×10.

Ceramic based nanocomposite, in which nano-sized ceramics and metals were dispersed within matrix grains and/or at grain boundaries, were successfully fabricated in the ceramic/cerarnic and ceramic/metal composite systems such as /SiC, /, MgO/SiC, mullite/SiC, /SiC, /B, /W, /Mo, /Ni and /Mo systems. In these systems, the ceramiclceramic composites were fabricated from homogeneously mixed powders, powders with thin coatings of the second phases and amorphous precursor composite powders by usual powder metallurgical methods. The ceramiclmetal nanocomposites were prepared by combination of H2 reduction of metal oxides in the early stage of sinterings and usual powder metallurgical processes. The transmission electron microscopic observation for the /SiC nanocomposite indicated that the second phases less than 70nm were mainly located within matrix grains and the larger particles were dispersed at the grain boundaries. The similar observation was also identified for other cerarnic/ceramic and ceramiclmetal nanocornposites. The striking findings in these nanocomposites were that mechanical properties were significantly improved by the nano-sized dispersion from 5 to 10 vol% even at high temperatures. For example, the improvement in hcture strength by 2 to 5 times and in creep resistance by 2 to 4 orders was observed not only for the ceramidceramic nanocomposites but also for the ceramiclmetal nanocomposites with only 5~01%se cond phase. The newly developed silicon nitride/boron nitride nanocomposites, in which nano-sized hexagonal BN particulates with low Young's modulus and fracture strength were dispersed mainly within matrix grains, gave also the strong improvement in fracture strength and thermal shock fracture resistance. In presentation, the process-rnicro/nanostructure-properties relationship will be presented in detail. The special emphasis will be placed on the understanding of the roles of nano-sized dispersions on mechanical properties.