In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

This study is to review its possibility of recycling, subject for Polyolefine (TPO) and Polyvinyl Chloride (PVC) among thermoplasticity plastic raw materials, of which recycling is possible. Wasted vinyl and processing by-products have been used for Polyolefine (TPO), and coating from used cable and its by-products used for Polyvinyl Chloride (PVC). The study result is shown as following. (1) In case of Polyolefine (TPO), superior property has been shown, when mixed with raw materials, as the property of by-products is excellent. For Polyvinyl Chloride (PVC), property in the same level as raw materials has been shown up to 50% of by-products mixing rates. (2) In case of coating from the used cable, the property as exactly same as raw materials without property loss has been shown since long time passed, with using highquality raw materials at that time of manufacture.

공간적 위치 및 유량 값에 따라 각 계산점마다 조도계수의 값이 달리 주어질 수 있도록 하는 가변 조도변수 부정류 계산모형을 수립하였다. 유량과 조도계수의 관계식으로는 계단함수 또는 멱함수를 적용할 수 있도록 하였다. 수립된 모형을 충주댐부터 팔당댐까지의 남한강 구간에 적용하여 최적화에 의한 매개변수의 추정을 수행하였다. 가변 매개변수 모형의 보정 결과, 계단함수 도형 및 멱함수 모형 모두 유량이 커질수록 조도계수가 감소하는 경향이 일관되게 나타났다. 이

2000년 2월부터 2001년 2월까지 남태평양 미크로네시아 군도의 Chuju 연안에 서식하는 주Epinephelus merra 암컷을 대상으로 생식주기를 조사하였다. 생식소숙도지수 (GSI)는 2월부터 증가하기 시작하여 3월에 최고치 (3.410.84)를 보였다. 조직학적 관찰결과 3월과 4월의 난소 안에는 난황을 가진 다양한 단계의 난모세포와 배란여포들이 존재하였다. 6월부터 1월에 난소 안에는 미성숙 난모세포만 있었다. 이들 결과로 E. merra