Deoxygenation process in which CBOD(carbonaceous BOD) is biochemically oxidized to reduced inorganic compounds is of great significance in the oxygen demand of stream waters. The CBOD decay rate has traditionally been determined in a laboratory by CBOD bottle incubation method. But in this study, CBOD decay rates were obtained by laboratory incubation method and natural waters experiment. Average CBOD decay rate for the Nakdong river(upper zone) in natural waters were 0.553 day-1 during April 2005 to January 2007. The values in the middle and down parts of the Nakdong river in natural waters were 0.384 day-1 and 0.252 day-1 at the same period of time, respectively. Average CBOD decay rates using by incubation method in the upper/middle/down parts of the main stream in the Nakdong river basin was 0.270 day-1, 0.289 day-1, and 0.283 day-1 during April 2005 to January 2007, respectively.