Air curtains, blowing air streams across a doorway, were installed in trial trains on subway line 7 in 2021 and they have been operated with dust collectors to improve the air quality of subway trains. In this study, we investigated the effects of air curtains on the indoor air quality in the trial subway trains. The concentrations of PM2.5 and carbon dioxide in the four selected cabins were measured in the morning hours (e.g., 7:30–11:30 am including the morning rush hour). The measurements were conducted on February 26, March 31, April 30, and May 14, 2021 and air curtain (AC)s and dust collector (DC)s in the four cabins were operated differently on those measuring days. All devices were turned off in the control cabin and only ACs, only DCs, and both the ACs and DCs were turned on in the other three cabins, respectively. The 4-h-averaged PM2.5 concentrations in the cabins, where only ACs and only DCs are turned on, are lower than in the control cabin by 18% and 26%, respectively. In addition, the joint operation of ACs and DCs can decrease the PM2.5 concentration by up to 42%. The time series of PM2.5 concentrations, measured on April 30, illustrate again that ACs block the intrusion of outside particulate matters. The 4-h-averaged carbon dioxide concentrations in the four cabins do not show monotonic differences between the cabins because of the generation of carbon dioxides inside the cabins. When the weights of individual cars and thus the numbers of passengers are similar between the cabins, the carbon dioxide concentrations in the ACs-operated cabins are higher than in the control cabin. This indicates that ACs can block the outward emission of carbon dioxides and maybe other indoor air pollutants as well.