This study evaluates the ammonia (NH3) reduction effect of Bio-curtains (hereinafter referred to as curtains) utilized for odor control in pig farms based on the distance outside the curtain and the spraying condition. The curtain (total area: 37.9m3) was constructed with two layers of light-shielding screens stretched over a rectangular parallelepiped structure installed around a ventilation fan (630 mm) on the side wall of a pig barn where 48 finishing pigs were reared. The real-time NH3 concentration was measured by using a photoacoustic spectrometer. In the first part of this study, the NH3 measurement position for each side of the curtain was selected based on the lowest standard deviation among 4 to 8 initial sampling points on the surface of the curtain and utilized for both experiments of distance and spraying. In the experiment concerning the distance outside the curtain, ammonia concentration decreased by 17.45% at 2m compared to the distance at 5 cm and by 6.94% at 4m compared to 2m on average. In contrast, the NH3 reduction rate for each distance compared to the ventilation fan was the lowest at the 100% operating rate in which the exhausted NH3 concentration from the ventilation fan was low. At this time, the spraying mist on the inside of the curtain increased the NH3 reduction rate by 4.98 to 10.36% compared to the non-spraying condition. Consequently, the NH3 be reduced as distance outside the curtain increases due to the diffusion effect caused by the surrounding wind and the spraying mist on the inside curtain on the dissolution of NH3.