In order to establish the enzyme linked immunosorbent assay(ELISA) of sterigmatocystin produced by Aspergillus versicolor, we experimented and obtained following results. Two of three rabbits which had been immunized with sterigmatocystin-hemiacetal-BSA produced antibodies against sterigmatocystin at 15 weeks. The produced antibodies were specific for sterigmatocystin and sterigmatocystin-hemiacetal but didn't cross react with other sterigmatocystin analogues in a significant degree. DMF : 4% KCl (18:2) mixed solution was most effective to dissolve sterigmatocystin. For the preparation of sample solution to determine sterigmatocystin by ELISA, sample was extracted with CHCl₃ and dried, then the dried sample was redissolved with 100 ul DMF+4% KCl mixture. 10-1,000 ng/ml level. of standard sterigmatocystin could be applied to the established ELISA. When artifically contaminated rice were assayed by the ELISA, the average recovery of sterigmatocystin spiked to 25-500 ng/g was 109% (97-116%), and mean interwell coefficient of variation was 21% (11-28%).

고려인삼(Panax ginseng C.A. Meyer) 잎 중의 가용성 invertase를 탈이온수로 추출한 후 황산암모늄 0, 4∼0.6 포화획분에 의하여 조제하였으며 이의 효소화힉적 성질을 검토한 결과는 다음과 같다. 본 효소의 최적 pH와 온도는 pH 6.0과 40℃ 였으며 pH 6.0∼8.0 범위와 40℃ 이하에서 안정하였다. Protease 및 단백질 변성제 처리에 의하여 활성이 현저히 저하되었으며 glycosidase에 의해서는 저해를 받지 않았다. 본 효소는 sucrose와 inulin 등의 β-fructofuranoside 결합을 가진 기질에 특이적으로 작용하여 전형적인 β-fructofuranosidase의 성질을 나타내었다.