Selenium was initially considered toxic to humans, but it was then discovered that selenium is essential for normal life processes. Selenium plays important roles in antioxidants. It is expected that chitosan microcapsules containing nano-selenium will be able to be used as a key material in bio-medical and cosmetic applications. The high concentration of chitosan derivatives guaranteesincreased antioxidative activity. Both inorganic and organic forms of selenium can be nutritional sources. The antioxidant properties of selenoproteins help prevent cellular damage from free radicals. The objective of this experiment was to study the antioxidative activity of chitosan nano-selenium. Our experiments were divided into five groups, in the presence of various concentrations(0.1%, 0.3%, 0.5%, 0.7%, and 0.9%) of chitosan. We performed an assessment of the antioxidant properties and cytotoxicity of respective concentrations of chitosan nano-selenium. The antioxidant activity was examined by the free radical scavenging activity on 1, 1-diphenyl-2-picrylhydrazyl(DPPH) assay. The cytotoxicity effect was measured by means of 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay. As a result, the electron donating abilities of 0.1%, 0.3%, 0.5%, 0.7%, and 0.9% of chitosan nano-selenium exhibited effective andioxidant scavenging activity at 12.5 ㎍/㎖ against DPPH radicals. 0.3% chitosan nano-selenium did not show cytotoxicity on human keratinocytes. In general, the cytotoxicity of 0.1% and 0.9% chitosan nano-selenium showed the lowest effects. Though low cytotoxicity of 0.5% and 0.7% chitosan nano-selenium exhibited 29.67% and 38.4% against human keratinocytes on adding 100 ㎍/㎖ and 50 ㎍/㎖, respectively, cell vitality was recovered with 200 ㎍/㎖. These findings support the notion that chitosan nano-selenium may be useful as a new active ingredient source for bioactive compounds.

원형교차로 중 회전교차로는 기존에 존재하였던 로터리의 형태와 유사한 형태의 교차로로서 최근 세계적으로 많이 설치되고 있으며 우리나라에서도 회전교차로에 대한 관심이 증가하고 있다. 그러나 도시부의 경우 회전교차로 운영 효율에 큰 영향을 미치는 요소 중 하나인 보행량에 대한 영향 분석은 미미하다. 따라서 본 연구에서는 보행량에 따른 회전교차로의 효율 분석 및 보행신호를 제시하였다. 첫째, 도시부 1차로 회전교차로의 용량을 분석하기 위해 VISSIM 시뮬레이션을 구성하였다. 둘째, VISSIM 시뮬레이션을 통해 보행량에 따라 신호가 없는 회전교차로와 신호가 있는 원형교차로로 구분하여 교차로의 평균차량 지체를 산정하였다. 끝으로, 이 연구에서 나타난 분석결과 도시부 회전교차로 설치시 보행량이 200명/시 이하인 경우 회전교차로가 적합하며 보행량이 200명/시 이상인 경우는 펠리컨 신호가 있는 원형교차로, 보행량이 600명/시가 높으면서 교통량이 1,500대/시 이상인 경우는 정주기식 신호가 있는 신호교차로가 적합한 것으로 나타났다.