Rice is the staple food of at least half of the world's population. Due to global warming, the weather is difficult to forecast nowadays. Therefore, it is necessary to breed various breeding to respond to such changes in the environment. This study was conducted to analyze the QTL about plant form, culm length, ear number and ear length by using 120 lines by anther culture, a cross between the Indica variety Cheongcheong and Japonica variety Nagdong. DNA marker was selected on the QTLs gene, and the following results were obtained. CNDH (Cheongcheong Nagdong Doubled Haploid) lines frequency distribution table curves about culm length, ear number and ear length exhibited showed a continuous variation close to a normal distribution. QTL analysis result, on culm length qPlL1-1 and qPlL1-2 were detected on the chromosome 1 and qPlL5 was detected on the chromosome 5. However, on ear length qPL2, qPL3 and qPL10, were detected on the chromosome 2, 3 and 10, while on ear number qPN1-1 and qPN1-2 were detected on the chromosome 1, qPN9 was detected on the chromosome 9. The QTLs related to culm length was found to chromosomes 5 and LOD scores were 3.81. The QTLs related to ear length was found to chromosomes 2 and 3 LOD scores were 7.13 and 3.20. The QTLs related to ear number was found to chromosome 9 and LOD scores were 4.27. Twenty two (22) Japonica cultivars and 12 Indica cultivars were analyzed polymorphisms, using selected 9 markers from the result about plant form analysis. RM5311, RM555 and RM8111 about the culm length, the ear length and number of ear were selected on the standard of Cheongcheong and Nagdong. Each rate of concordances about the culm length, the ear length and number of ear are 44.11%, 41.17% and 44.11%.

본 연구는 사과의 자가불화합성 현상에 효과적인 저해제와 활력제를 탐색하기 위해 여러 종류의 물질을 조사하였다. 후지 사과의 화주조직에서 분비되는 S RNase에 대한 저해효과를 조사하기 위해 In vitro 상에서 효소 저해제를 넣어 효소활성을 측정한 결과, Iron(II) Sulfate에서 현저하게 활성이 24.3% 감소되어 저해효과가 뛰어난 것으로 나타났다. In vitro 상에서 후지 사과의 화분관 신장 억제 효과를 분석한 결과, 후지 사과의 화분은 2% Sucrose 신장배지 보다 10% Sucrose 신장배지에서 약 90% 이상의 높은 발아율을 나타났다. 또한 저해제와 첨가제 처리에 의한 안동지역과 영주지역의 Field에서 후지 사과 착과율 조사 결과, 안동지역에서는 A++V6 2회 처리가 86.1%로 중심화착과율이 가장 높았으며, 영주지역에서는 A++V1 1회 처리가 91.9%로 중심화 착과율이 가장 높은 것으로 나타났다.

The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.

사과 재배시 대두되고 있는 수정 불량 및 안정된 수정을 통한 품질 향상을 위한 방안을 모색하고자 자가불화합성 제어제(상품명 : 애플플러스)를 이용한 SSR 유전 분석을 통한 자가수정 향상 정도 및 촉진 효과, 품질 특성에 미치는 영향을 검토하였다. 1. 후지 품종에 있어서 중심화 결실율은 자연방임시 무처리의 경우 2004년 0.5%, 2005년 3.0%인데 비하여 자가불화합성 제어제 처리의 경우 2004년 17.2%, 2005년 44.4%로 높게 나타났다. 2. 망을 씌워 자가불화합성 제어제를 처리한 실험구에서도 무처리구에서는 2004년 0.4%, 2005년 0.7%, 처리구에서는 2004년 3.3%, 2005년 5.2%의 결실율을 보여 처리구가 7.4~8.3 배정도 결실율이 높게 나타났다. 3. 망을 씌워 자가불화합성 제어제 처리한 실험구와 무처리구를 비교하면 L/D, 경도에서는 유의성이 인정되지 않았으며, 종자수에서는 무처리구 0.9개, IS-1 처리구 1.2개, IS-2 처리구 1.2개, IS-3 처리구 1.5개, IS-4 처리구 0.7개로 나타나 약제 종류에 따라 효과가 다르게 나타났다. 4. SSR 유전자 분석에 의한 후지 품종 종자의 자가수분 정도는 무처리구에서 0%, IS-1 처리구 100%, IS-2 처리구 73%, IS-3 처리구 88%, IS-4 처리구 62%로 나타나 IS-1의 약제가 가장 높게 나타났다.