This study was conducted for recycling the waste MDF(Medium-density fibreboard) to investigate the enzymatic saccharification characteristics using two enzymes Novozyme Cellic® CTec2 and HTec2 (Novozymes, Bagsvaerd, Denmark) after the delignification by pretreatment using sodium chlorite. The chemical composition of the waste MDF are lignin, holocellulose, ash, and other extracts 28.40, 60.20, 0.10, and 11.30%, after pretreatment with sodium chlorite were 5.20, 53.10, 0.03, and 41.67%. The Lignin interferes with enzymatic saccharification of 23.2% was removed, 7.1% of holocellulose was lost. The times of sodium chlorite pretreatment and saccharification of the waste MDF was finished between 48-72 hours, the saccharification speed was fast when the concentration of the enzyme by 10% and the HTec2 CTec2 ratio 9:1. Sugar ratio of the solid content of the waste MDF is the highest as 69.6% when it comes out of 8% and a viscosity was as high as 34.8% when the 12-FB%. Therefore, the pre-treatment with sodium chlorite is more advantageous when enzymatic saccharification to lignocellulosic biomass. The amount of the enzyme, the solid-liquid ratio, and the reaction time showed a proportional relationship with saccharification efficiency. The studies for increasing the solids content of waste MDF to improve the economic efficiency more than 12% should accompanied.