DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.

  This study is concerned with image information management system for small scale hospital. We intended to developing image management system which is used of the existing analog devices such as medical camera, supersonic analyser, endoscope etc. We deve

Trouble prevention of facilities in operation process plays an Important role for Improving facilities productivity as pro-duct-on systemization is installed with development of facilities automatization Condition monitoring predicts machine's in-ternal c

This study is concerned with image information management system form small scale hospital. We intended to developing image management system which is used of the existing analog devices such as medical camera, supersonic analyser, endoscope etc. We developed a video in interface board based USB 2.0 for handling image in real time. It is capable of transmitting image input signal like as NTSC, PAL to personal computer through USB 2.0. The developed board can transmit image up to 40times speed compared with the exiting system based USB 1.0. Especially the developed system is very helpful for small hospital like as dental clinic, because it is easy and convenient to manage image information without expert. So it will provide reduction of time and cost for handling image information(collecting, saving, retrieval, transmitting image).

The official ebXML white paper states that the vision of ebXML is to create a single global "electronic marketplace" where enterprises of any size and in any geographical location can meet and conduct business with each other through the exchange of XML based messages. ebXML is an end-to-end solution between ebXML compliant software. However, B2B e-marketplace business models do not include such end-to-end B2B models that trading partners exchange XML or EDI based messages with each other. Therefore, this paper aims to clarify the difference between ebXML and B2B public e-marketplace as well as to discuss the application of ebXML to public e-marketplace for making a right decision to adopt a new B2B e-business technology.

This paper presents the business model and system functionalities of a B2B e-marketplace solution that not only can overcome some problems of the public e-marketplace, but also can be applicable to both public and private types of e-marketplaces. This sol