The levels of sulfites in wooden chopsticks were analyzed using two different methods, distillation- alkali titration and ion chromatography. According to the two methods, Sulfur dioxide (SO₂) was validated by 0.6 and 0.2 μg/ml of limit of detection (LOD), 1.8 and 0.5 μg/ml of limit of quantification, (LOQ) and a R² > 0.998 for linearity, respectively. The recoveries of SO₂ from food simulants spiked ar levels of 100 ppm were 70.2-100.2%. 158 samples of wooden chopsticks were monitored the migrated amounts of sulfites. Sulfites were detected in 30 samples in ranged of 0.6 to 15.5 ppm. Maximum migration level, 15.5 ppm of sulfites was considered giving no harmful effect to human since it was reached 5.5% of estimated SML.