Background : Korea's agriculture has been forced to change due to the decline in agricultural population, the aging of the population and the conclusion of the Convention on Biological Diversity. Thus, agriculture is seeking to develop into a sixth industry. For this reason, it is necessary to find high-function alternative plant resources for health promotion to meet the changes in national needs for agricultural products. The adventitious roots culture of wild mountain ginseng is the raw material of food code that produced by the biotechnology technique. introduced in the culture of the plant, which is the only way to use a raw material of food. Therefore, in order to increase the yield of ginsenoside in adventitious roots culture of wild mountain ginseng, ginsenoside precursor was treated and ginsenoside contents were analyzed. Methods and Results : In order to investigate effect of precursor treatment on the production of ginsenoside in adventitious roots culture of wild mountain ginseng, 5 g of adventitious roots culture of mountain ginseng were cultured in liquid SH media supplemented with 2 ㎎/l IBA, 3% sucrose for 8 weeks, which was co-cultured with β-sitosterol and Squalene (0.0625 to 1 mM) or without. Determination of 17 ginsenosides contents of each extract was carried out by HPLC. Rb3 was accumulated by only β-sitosterol, also it increased production of total ginsenoside in the cultured ginseng roots at a concentration of 0.125 mM, which was 2.47-fold higher than that in the control (78.13 ㎎/g of extract). Conclusion : These results are an important to improve the production yield of ginsenoside in adventitious roots culture of wild mountain ginseng, and they provide an opportunity for development of adventitious roots culture of wild mountain ginseng in dietary health supplement and pharmaceutical industries.

Background : Collagen and elastin contribute to form a network under the epidermis and reduce the wrinkles. Collagen and elastin are degraded by collagenase and elastase. Therefore, inhibition of collagenase and elastase activity could be an excellent method for anti-wrinkle. Pinus Koraiensis leaves contain various flavonoid compounds such as quercetin and kaempferol. According to previous reports, kaempferol has increases procollagen synthesis and inhibitory effect on MMP-1 activity. This study was performed to investigate the anti-wrinkle effect of Pinus Koraiensis leaves extract and fractions. Methods and Results : The active components of Pinus Koraiensis leaves extracts and fractions were determined. Total phenol and total flavonoid contents measured using the Folin-Ciocalteu reagent and according with aluminum chloride colormetric method. The kaempferol content analysis using HPLC system. The total phenol, flavonoid and kaempferol contents of ethyl acetate fraction was the highest. The antioxidant activity measured in according with DPPH, ABTS and RP assay. Pinus Koraiensis leaves extract and factions were the highest ethyl acetate fraction in antioxidant activity. The inhibitory activity on collagenase and elastase of Pinus Koraiensis leaves extract and factions were determined. The results of inhibitory activity on enzymes associated with wrinkle formation was the highest of n-butanol fraction, the next higher was 80% ethanol extract. As a result of using MMP-1 content and procollagen type-I C-peptide content assay kit, the anti-wrinkle effect of n-hexane fraction was highest. Conclusion : These results indicate that Pinus Koraiensis extract may help anti-wrinkl