Gluten proteins play a key role in the unique baking quality of wheat by determining the water absorption capacity, cohesivity, viscosity, and elasticity of the dough. However, gluten from wheat, barley, rye, and oat can induce gluten sensitivity as well as celiac disease in susceptible populations. Hence, the gluten levels in foods labeled “gluten free” should be monitored. In this study, gluten-containing samples (sample: 600 g, water: 390 g) were treated with the commercial enzyme Protamex® (0.1-0.3% of sample weight) for 1-4 h and then measured with three ELISA kits. In the more viscous sample after treatment with 0.1% Protamex® for 1 h, the measured gluten contents were 1,802.6, 1,718.6, and 1,698.7 mg/kg using the G12, GLUTEN-CHECK, and Wheat/gluten (Gliadin) ELISA kits, respectively. The sample treated with 0.3% enzyme for 4 h had a lower viscosity (32.2 cps), and all three kits gave its gluten content as around 8.4 mg/g. When gluten breaks down, it does not act as gluten and its degradation is due to the enzyme. However, even when Protamex® was used at the same concentration for the same time, the measured values seem to be different for samples with and without the final heating treatment.

In this study, β-1,3/1,6-glucan, lactic acid bacteria, and β-1,3/1,6-glucan+lactic acid bacteria were tested for 10 weeks using an immunodeficient animal model infected with LP-BM5 murine AIDS virus On the immune activity. Cytokines production, plasma immunoglobulin concentration, T cell and B cell proliferation were measured. As a result, the T cell proliferative capacity which was weakened by immunization with LP-BM5 murine AIDS virus increased significantly T cell proliferative capacity compared with the red ginseng control group. B cell proliferative capacity was significantly higher than the infected control group. Increased B cell proliferation was reduced. In the cytokine production, IL-2, IL-12 and IL-15 in the Th1-type cytokine increased the secretion of IL-2, IL-12 and IL-15 compared to the infected control. The proliferative capacity of the treated group was higher than that of the mixed treatment group. TNF-α was significantly decreased compared with the infected control group. The IL-4, IL-6 and IL-10 levels were significantly inhibited in the infected control group and the Th1/Th2 type cytokine expression was regulated by immunohistochemistry. IgE, IgA, and IgG levels were significantly lower in the immunoglobulin secretion assay than in the control. As a result, the immunomodulatory effect of β-1,3/1,6-glucan+lactic acid bacteria was confirmed by mixing with LP-BM5 murine AIDS virus-infected immunodeficient animal model.

대나무 죽력의 ACE 저해효과와 마우스모델에서의 혈행 개선 효과를 보았다. 혈전용해능에서 청국장추출물은 2.56 unit, 미정제죽력에서 4.71 unit, 정제죽력에서 4.32 unit를 나타내어 모두가 plasmin 보다 유의하게 높은 활성이 높고, ACE 활성 저해 효과에서는 죽력 원액, 미정제 죽력과 정제 죽력에서 각각 70.25%, 69.01%, 68.23%로 대조군으로 사용된 청국장이 26.56%으로 나타나 높은 억제효과를 보였다. 마우스모델시험에서 고지방식이군과 함께 고지방식이군+ 정제죽력 0.01%와 0.05%를 투여, 혈청 중 지질변화에서 triglyceride의 함량은 고지방식이군이 일반식이군(271.1 mg/dL)에 비해 314.4 mg/dL로 증가하였고, 고지방식이+죽 력 0.01%군은 301.5 mg/dL로 나타났으며, 고지방식이+죽 력 0.05%군은 289.2 mg/dL으로 유의하게 중성지질이 감소를 나타내었고, total-cholesterol 함량은 327.2 mg/dL로 증가 하였으며, 고지방식이+죽력 0.01%군은 311.9 mg/dL로 나타났으며, 고지방식이+죽력 0.05%군은 293.7 mg/dL로 고 지방식이군에 비교, 유의하게 total-cholesterol이 감소세를 나타내었다. 한편 HDL-cholesterol은 일반식이군이 206.0 mg/dL로 가장 높은 값을 나타내었으며, 고지방식이+죽력 0.01%군 196.6 mg/dL, 고지방식이+죽력 0.05%군 189.2 mg/dL, 고지방식이군 162.2 mg/dL순으로 나타났고, 죽력첨 가군 0.01%군과 0.05%군간의 유의성은 없었다. 혈류개선 효과에서는 고지방식이에 비하여 고지방식이+죽력 0.05% 군이 고지방식이군, 고지방식이+죽력 0.01%군보다 모두 유의하게 빠르게 모세관을 통과하여 빠른 혈액 유동성을 나타내었다. 혈소판응집 억제능에서는 고지방식이군의 경우 대조군인 일반식이군에 비해 혈소판 응집이 증가한 반면 고지방식이+죽력 0.01%군과 고지방식이+죽력 0.05%군은 고지방식이군에 비해 혈소판응집이 억제되는 것으로 나타났다.

β-Glucan is a natural compound contained in cell walls of yeast or fungi, and cereal’s fiber. It is also known to boost the immune system in human. Aureobasidium is a producer of water-soluble β-1,3/1,6-glucan. In this study, natural killer (NK) cell and macrophage activity were tested to investigate the effects of β-1,3/1,6-glucan isolated from A. pullulans on immune activity. Activation of NK cell was increased about 63-39% by the treatment of 10-200 μg/mL β-1,3/1,6-glucan than control. Besides, only 10 μg/mL of β-1,3/1,6-glucan was enough to boost activation of NK cell. Phagocytosis of macrophage was increased to 15~21% by the treatment of 10~200 μg/mL of β-1,3/1,6-glucan than zymosan-treatment. In LP-BM5 proliferating inhibition test, relative mRNA level of LP-BM5 virus was decreased in β-1,3/1,6-glucan-treated cell about 36~74% than control. The decline of LP-BM5 mRNA level appeared to depend on the concentration of β-1,3/1,6-glucan. These results suggest that pure β-1,3/1,6-glucan from A. pullulans might be contributing to enhancement of immune activity through the activation of NK cell and phagocytosis of macrophage. Moreover, treatment of the β-1,3/1,6-glucan could increase the resistance to virus infection such as LP-BM5 through the restraining of the multiplication.

A new lactic acid bacteria with gluten-degrading activity which was isolated from salted sea foods (traditional Korea fermented food), identified as Weissella confusa (99%) by use of API kit and 16S rRNA sequencing, and designated as W. confusa. When the W. confusa cultured for 48 hours at 30℃ in a MRS medium containing 1% gluten, 45% of gluten was founded to be degraded. W. confusa showed 85% of survival rate at pH 3, and 94% tolerance at 0.1% oxgall, which indicates that W. confusa would survive in stomach of human. Experiments on the thermostability was confirmed that it has a stability of 70% in 50℃. W. confusa inhibited the growth of some pathogen, except for S. aureus. Results in this study suggest that using W. confusa for fermentation of grain flour containing gluten would be desirable to prepare the gluten-free foods needed for those who suffer from celia disease and gluten allergy.

본 연구에서는 사용이 증가되고 있는 천공포장재 필름을 방울 토마토 저장에 이용하여 방울 토마토의 품질변화를 조사하였으며, 또한 세척 및 꼭지 제거의 최소가공 처리유무가 방울 토마토의 저장성에 미치는 영향 및 최적 천공포장 조건을 살펴보았다. 에틸렌 가스 및 이산화탄소는 천공필름 중 투과성이 가장 낮은 LF05 및 OPP/PE 필름구에서 상대적으로 투과성이 높은 LF10, LF20, LF40에 비해 높은 값을 보였고, 산소 분압의 경우 LF05에서