Background : Currently sprout ginsengs are produced in extensive variations of cultivation times, methods and specifications that are different in each farm in Korea. Also, regardless the effective ingredient content of ginseng, overgrown sprout ginseng are distributed so that the quality standardization of sprout ginseng is urgently required. The sprout ginseng market is only a segment of the Korean ginseng market still. However, If development of processed products utilizing the functionalities is connected, the market may exponentially grow. Also, if the use of sprout ginseng is extended to productions of drinks, cosmetics, facial masks and others in the cosmetics and health functional food industries, the export competitiveness to the global market is expected to increase. Therefore, this study aims the quality standardization of sprout ginseng to secure the market competitiveness in Korea and overseas by examining the property change following the cultivation phases. Methods and Results : For the overground growing in green houses, the sprout ginsengs planted in early April and early June show to be better in growing than the ones planted in early August. For the underground growing, while no significance is found among the cultivation periods, the sprout ginsengs planted in early June are excellent in terms of the root length and the weight of overground portion. Also, according to the examination on the stem properties of 2-year grown sprout ginsengs in each cultivation time, a trend that, as the growing period is longer from 25 days to 35 days, 45 days and 95 days, the hardness, gumminess, chewingness and springiness of stem are higher. And, on the hardness, gumminess, chewingness and springiness of leaf, while no statistically significance is found in the growing periods from 25 days to 45 days, the 95-day sprout ginsengs show high figures referring that the leaves becomes tougher. Conclusion : In case of cultivating sprout ginsengs in a green house, cultivating in April - June is advantageous for the overground and underground growing. And, it is determined that cultivating sprout ginsengs for 35 - 45 days produces leaves and stems with the most excellent chewing textures felt by consumers when chewing.

This study was carried out to investigate shelf-life and quality of fresh-cut Dungkunma (Dioscorea bulbifera) in order to elevate utilization of Dungkunma a fresh food. Before vacuum-packaging (in polyethylene/polypropylene film (100μm, 15×20 cm, 75±2 cmHg) and storaging at 2℃, Dungkunma was peeled out and cut to dice type (2.0±0.5 cm3), and then washed and blanched using hot water (at 90±2℃ with 2% NaCl solution for 30 sec). Blanched Dungkunma was pre-dried at room temperature, 40℃ and 50℃ for removing surface water. Each peeled dice Dungkunma was packed 50 g in polyethylene/polypropylene film (100 μm, 15×20 cm) with vacuum treatment (75±2 cmHg) and stored at 2℃ for 90 days. Hardness and adhesiveness of Dungkunma blanched by 2% NaCl and pre-dried at 50℃ (SB50) were the highest, but changes were the least during storage. Lightness and yellowness of stored Dungkunma in all treatments decreased slightly while redness increased during storage. Changes of color of SB50 was the least. Total concentration of aerobic bacteria in SB50 was 1.88±0.18 log CFU/g during 90 days and E. coli was detected in all treatments during whole storage periods. Dioscin and allantoin contents of SB50 were virtually unchanged during the storage. Consequently, the results of this study suggest that vacuum packaged Dungkunma after blanching using 2% NaCl solution could be effective to prolong the quality of fresh-cut Dungkunma.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : Tetraploid lines and maternal varieties used in this study were C0148-10 and C0412-1 from Cheongyang-jaerae, M0148-94 and M0148-120 from Myongan, B0148-43 and B0148-78 from Bulro, D0148-62 and D0148-72 from Cheongdae, and Y0148-2 and Y0148-24 from Youngha. For organic acid composition of tetraploid lines and matrenal varieties, malic acid was highest as 1.47 – 4.6 ㎎/g in fruit, and citric acid and succinic acid were highest in leaf as 2.67 – 4.08 ㎎/g and 4.28 – 6.00 ㎎/g. Total organic acid content in root ranged from 1.78 to 3.23 ㎎/g, lower than in fruit and leaf. Of 11 fatty acids composing the boxthorn fruit, linoleic acid was highest as 25.36 – 50.33 ㎎/g. For leaf, linolenic acid was highest as 4.39 – 8.77 ㎎/g. Linoleic acid was highest as 1.65 – 6.98 ㎎/g of all fatty acids in root. 19 free amino acids were analyzed. Average content of essential amino acids in fruit was 6.64% and lysine was highest as 1.57%. Non-essential amino acid content was 8.26% and serine was highest as 2.72% of all non-essential amino acids in fruit. D0148-62 was highest in the total amino acid and the essential amino acid as 23.58% and 10.19%, respectively. Total amino acid content in leaf was 26.49%. Essential amino acid was 12.12% and leucine was highest as 2.08%. Non-essential amino acid was 14.37% and serine was highest as 4.61%. Total amino acid content in root was 13.25%. Essential amino acid was 6.66% and arginine was highest as 2.58%. Non-essential amino acid was 6.59% and serine was highest as 2.60%. Conclusion : Organic acid content increased in fruit of tetraploid lines and lines induced from Cheongyang-jaerae, Myongan, and Cheongdae were higher in contents of linoleic acid, oleic acid and palmitic acid, resulted in total fatty acid increasing. This shows several induced tetraploid boxthorn lines are very useful resources in breeding new varieties.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : Tetraploid lines and maternal varieties used in this study were C0148-10 and C0412-1 from Cheongyang-jaerae, M0148-94 and M0148-120 from Myongan, B0148-43 and B0148-78 from Bulro, D0148-62 and D0148-72 from Cheongdae, and Y0148-2 and Y0148-24 from Youngha. Betaine content was highest as 0.7 - 1.62% in leaf, followed by 0.55 - 1.17% in fruit and 0.04 - 0.23% in root. Betaine content in plant parts of several tetraploid lines increased compared to martenal varieties, higher in fruit for 5 lines including D0148-72, B0148-78, and C0142-1, higher in leaf for 5 lines including C0148-10, C0412-1, and M0148-94, and higher in root for 7 lines including Y0148-2, M0148-94, and M0148-120. Rutin content in leaf ranged 4.0 – 388.55 ㎎% and was highest as 388.55 ㎎% in Y0148-24. Tannin content in leaf of tetraploid lines was 4.70 - 6.12%, highest as 6.12% in Y0148-2 and M0148-120, similar to the maternal varieties. Youngha of the diploid plants showed the highest tannin content of 7.08%. Total free sugar content in tetraploid lines was higher as 8.53 - 12.53% than maternal varieties. Conclusion : Betaine and rutin contents increased in several tetraploid lines and Total free sugar content in tetraploid lines was higher as 8.53 - 12.53% than maternal varieties. This shows tetraploid boxthorn lines are very useful resources in breeding new varieties.

Background : Dungkunma (Dioscorea bulbifera, commonly known as the air potato, air yam, bitter yam, cheeky yam, potato yam) is a species of true yam in the yam family, and has been used as folk remedy to treat conjunctivitis, diarrhea and dysentery, etc. This study was carried out to investigate shelf-life and quality of fresh-cut Dungkunma (Dioscorea bulbifera) in order to elevate utilization of Dungkunma as fresh food. Methods and Results : Before vacuum-packaging (in polyethylene/polypropylene film (100 ㎛, 15 × 20 ㎝, 75 ± 2 ㎝Hg) and storage at 2℃, Dungkunma were peeled out and cut to dice type (2.0 ± 0.5 ㎤), and then washed and blanched (30 sec at 90 ± 2℃ hot water and 2% NaCl solution) and pre-dried at room temperature, 40℃ and 50℃ for removing surface water. Each peeled dice Dungkunma were packed 50 g in polyethylene/polypropylene film (100 ㎛, 15 × 20 ㎝) with vacuum treatment (75 ± 2 ㎝Hg) and stored at 2℃ for 90 days. Hardness and adhesiveness of Dungkunma blanched by 2% NaCl and pre-dried at 50℃ (SB50) was the highest and increased and decreased, respectively, but changes was the least during storage. Lightness and yellowness of stored Dungkunma in all treatments decreased slightly and redness increased during storage but changes of color was the least at SB50. On vacuum packing, SB50 showed 1.88 log CFU/g of total aerobic bacteria during 90 days, and E. coliwas detected in all treatments during whole storage periods. Dioscin and allantoin content of SB50 was virtually unchanged during the storage. Conclusion : Consequently, the results of this study suggest that vacuum packaged Dungkunma after blanching at 2% NaCl could be effective to prolong the quality of fresh-cut Dungkunma and could be easily used.

Background : In this study, a total of 46 breeding lines consisting of native ginseng collections from Geumsan was analyzed and clustered for the selection of Geumsan native ginseng in Korea using DNA markers. Methods and Results : We collected 46 Ginseng breeding lines from Geumsan : GS97-25 - GS00-58. Analyses of the genetic characteristics of the collection were conducted for extraction gDNA using sprout. 46 Ginseng breeding lines from Geumsan could be identified polymorphism using the selected 5 primer. We determained that the 46 breeding lines analyzed could be classified into 5 groups with similartiy value of 0.77 in dendrogram derived from the cluster analysis based on STS-markers. Group 4, which is the largest one, contained 19 collertions (41%). Conclusion : These finding could be used for morphological and genetic characteristics for produced native ginseng in Geumsan area. Futhermore, We could be used diverse genetic resources for Ginseng breeding.

Background : For cultivation of varieties of ginseng, the pure line selection method, which is to select the best among those cultivated in farms for pedigree breeding, replicated yield trials and regional adaptation trials before registering as a new variety, is widely used. Although there are 25 registered varieties of ginseng in Korea, the quality of ginseng is declining together with the amount of harvest being decreased by 15 - 20% due to the heat injuries and diseases from the warming & abnormal climate. Thus, the needs for development of disaster-resistant varieties with better chances of surviving through high temperature, salts and disease are increasing. Therefore, this study is to cultivate disaster-resistant varieties among those selected for their disaster tolerance and salt tolerance through regional adaptation trials. Methods and Results : As a result of examining the growth characteristics of the selected 2 - 5 year old varieties used in the study, among the 5-year old crops, Goryeo 4 and Eumseong 5 showed superior growth in both above and below aerial parts, and among the 4-year old crops, Eumseong 11 and Cheonryang showed superior growth while the growth in the below aerial parts were satisfactory in the order of Cheonryang > Eumseong 10 > Eumseong 11 > Eumseong 9. Among the 3-year old crops, the most superior growth in both above and below aerial parts was observed in Eumseong 14 with the weight of the below aerial part, root diameter and taproot length at 13.8 g, 11.8 ㎝ and 6.2 ㎝ respectively. Among the 2-year old crops, Eumseong 10 showed the most superior growth in both above and below aerial parts. Conclusion : Based on the above results, Goryeo 4 and Eumseong 5 among the 5-year old crops, Eumseong 11 among the 4-year old crops, Eumseong 14 among the 3-year old crops and Eumseon 10 among the 2-year old crops showed the most superior growth among the selected varieties. The growth characteristics of both above and below aerial parts in each year will continuously be monitored.

Background : Bai Zhu (Atractylodes macrocephala Koidz.) has been generally harvested in 1-2 years after seedstock planting. Recently, seed is used increasingly for planting in spring and rhizome is harvested in 1 year. In 1 year open field culture, seed harvesting rate is low below 30% and supply and demand of seed is so hard. This study was carried out to improve seed harvesting for stable seed supply and demand in Atractylodes macrocephala Methods and Results : Seed of Atractylodes macrocephala Koidzumi was directly sown in open field, under tunnel and rain shielding condition in mid-April. Tunnel was installed from prebloom, mid-september to seed harvesting time, November using 240 ㎝ wires for 120 ㎝ by interval and 20 ㎝ by height in furrow to assure insect pollination and wind fertilization. In rain shielding condition, plant height was highest as 38.2 ㎝ and number of branches, number of nodes, and content of chlorophyll were higher as 7.8 ea/plant, 8.7 ea/plant, and 65.4 respectively compared to open field and under tunnel. Flowering time was earliest as 23rd September in rain shielding condition and in open field and under tunnel was 26th September. There is no blossom rot in rain shielding condition while blossom rot occurrence was highest in open field by 5 in degree. Seed setting rate was highest as 42.0% in tunnel cultivation and seed weight per plant and 1,000 seed weight were highest as 3.7 g/plant and 22.3 g respectively. Total seed yield was higher as 58% and 65% in tunnel and rain shield condition respectively compared to 29 ㎏/10 a of open field. Tunnel cultivation for seed production of Atractylodes macrocephala is more beneficial for lower installation cost. Conclusion : Tunnel cultivation for seed production of Atractylodes macrocephala is more beneficial due to low blossom rot degree by 1, higher 1,000 seed weight and seed setting rate as 19.0 g and 42%, increased seed yield, and lower installation cost.

Background : In the year 2015, the area under rhizome of Rehmannia glutinosa in Chungnam province increased to 65% (105 ha) of domestic total cutivation area and is recognized as income crop. Since proper strorage method of rhizome has not been established, rhizome decay rate is so high during storage. This experiment was carried out to establish proper strorage method for stable production of rhizome of Rehmannia glutinosa Methods and Results : Korea jiwhang was used for this experiment and rhizome was cured under vinyl house and room temperature for 1, 2, 3 days respectively. Rhizome was stored for 140 days at –2 - 0℃ after curing, and rhizome quality and saprogen were investigated after storage. Trimming loss was 11.3 - 21.9%, higher in longer curig time and rice hull packing. Moisture content was 70.0 - 75.1%, higher in shorter curing time and no rice hull packing. Curing efficacy was better in vinyl house than room temperature, decay rate by weight and number of rhizome was lowest in 48hrs curing and no hull packing as 0.5% and 2.0% respectively. Identified saprogen during storage was Fusarium oxysporum, Fusarium tricinctum, Penicillium verrucosum, Mucor racemosus. Conclusion : Curing efficacy was better in 48hrs treatment under vinyl house with lowest decay rate and rice hull packing increased decay rate considerd due to saprogen existing in rice hull. 4 saprogens including Fusarium oxysporum were identified during storage of rhizome.

Background : In recent, a nationwide decrease in ginseng cultivation area and production is observed, and as the sales of ginseng decreases due to the economic downturn, the ginseng industry in Korea is going through more and more difficulties. However, sales of young ginseng, which are ready for the market after a short-term cultivation - 25 to 45 days - of 1 year old ginseng seedlings or 2 - 3 year old young ginseng in greenhouses and plant factories without using any chemical fertilizers, in large grocery chains and online shops is increasing. However, proper studies on cultivation of young ginseng in greenhouses are yet to be conducted, and thus, experiencing several issues including the cost burden of bed soil and disease occurred from reuse of the soil. Therefore, this study is to provide solutions for farmers cultivating young ginseng by determining the optimal type and amount of bed soil for box culture. Methods and Results : To determine the optimal type of bed soil for cultivation of young ginseng, six types of bed soil were produced by blending bed soil specialized for ginseng, granite residual soil and leaf mold in different ratios. To determine the optimal amount of bed soil for box culture of young ginseng, 1 year old ginseng seedlings are planted in three boxes - each in size of 52 ㎝ × 36.5 ㎝ × 9 ㎝ (W × L × H) filled with soils of 6 ℓ, 7 ℓ and 8 ℓ for an experimentation. Growth and development of 45-day old young ginseng showed satisfactory progress in the following order: 100% commercially available bed soil > 80% commercially available bed soil + 20% granite residual soil > 60% commercially available bed soil + 20% granite residual soil + 20% leaf mold, while the gross weight of below-aerial parts showed the same result. Conclusion : The optimum type of bed soil for greenhouse cultivation of young ginseng is the commercially available bed soil specialized for ginseng cultivation. However, the young ginseng showed relatively satisfactory growth and development in mixed bed soil - 60% commercially available bed soil + 20% granite residual soil + 20% leaf mold - and the growth and development of young ginseng were most satisfactory when the box (52 ㎝ × 36.5 ㎝ × 9 ㎝, W × L × H) was filled with 8 ℓ bed soil.

Background : The main ingredients of bed soil specialized for ginseng, which is often used in cultivation of young ginseng, are peat moss, vermiculite and cocopeat. These ingredients are imported from overseas countries because they are not produced in Korea, and thus play an important role in pricing of the bed soil. The bed soil products show satisfactory ability to retain moisture but, are known to require different containers and water management methods depending on the components of the soil and the crops to be cultivated. However, proper studies on cultivation of young ginseng in greenhouses are yet to be conducted, and research data on water management methods and irrigation amount for box culture of young ginseng are largely unavailable. Methods and Results : To establish the proper method of irrigation for cultivation of young ginseng, young ginseng were cultivated while providing water once a week through the method of drip irrigation and the method that combines drip irrigation and sprinkler irrigation. To establish the proper amount of water required, each box is provided with 2 ℓ, 3 ℓ and 4 ℓ of water respectively and the growth and development of both above and below aerial parts were examined after 45 days. The growth and development of both above and below aerial parts of young ginseng did not show any statistically significant differences between the two irrigation methods but, the young ginseng provided with water through the method that combines drip irrigation and sprinkler irrigation showed a relatively superior growth. Conclusion : Based on the above results, the proper method of irrigation for greenhouse cultivation of young ginseng is to combine the sprinkler irrigation - before budding - and the drip irrigation - after budding. The young ginseng showed the most satisfactory growth when provided with 4 ℓ water once a week, while those provided with less water – 2 ℓ - showed poor growth. For short-term cultivation of 45 days, the growth and development of young ginseng showed little difference regardless of the irrigation methods and the amount of water provided.

Background : With the recent increase in the area of ginseng cultivation in greenhouses, demand for studies of proper cropping patterns in direct seedling of ginseng and varieties of cultivation are increasing, and the needs for clean production of quality ginseng in greenhouses are emerging in connection with the consumer demand for pesticide-free ginseng and the supply and demand of ginseng seedling for cultivation of young ginseng. Therefore, this study is to provide baseline data in improving of quality of ginseng cultivated in greenhouses by examining the contents of ginsenoside in ginseng cultivated in different cropping patterns and different varieties. Methods and Results : As a result of examining the contents of ginsenoside in 3-year old, direct seedling ginseng cultivated in greenhouses in different depths – 10 ㎝, 20 ㎝ and 30 ㎝ - and width – 90 ㎝, 100 ㎝, 110 ㎝ and 120 ㎝ - of furrows, the contents of ginsenoside showed little differences by the depths of furrow in the furrow width of 90 ㎝ but, the highest content of ginsenoside Rb1 was observed in the furrow width of 120 ㎝ and depth of 20 ㎝, while the ginsenoside contents were high in the furrow depth of 20 ㎝ and width of 100 ㎝ and 120 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were greater in the following order: Geumgin > Geumsun > Chunpoong, while the contents of ginsenoside Rb1 were observed to be superior in Sunhyang, Gopoong and Geumseon and the contents of ginsenoside Rb2 were greater in the order of Geumgin > Guemsun >　Cheonpoong. Conclusion : Based on the results, the contents of ginsenoside in different cropping patterns of direct seedling cultivation in greenhouses were higher in lower depths of furrow - 10 ㎝ and 20 ㎝ - at furrow widths wider than 100 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were higher in varieties of Geumjin, Geumsun and Chunpoong.