자생 부추속 식물 중 강부추(Allium thunbergii for. rheophytum ined.)와 갯부추(A. pseudojaponicum Makino)는 관상용, 식 용 및 약용자원으로 가치가 있으나 육묘를 위한 생육환경조건 구명이 미비하여 연구할 필요성이 있다. 본 연구는 강부추와 갯부추의 육묘에 미치는 플러그 셀 크기, 차광률, 시비처리에 따른 영향을 구명하기 위하여 실험을 수행하였다. 강부추와 갯부추를 육묘한 결과, 플러그 셀 크기에서는 50, 72, 105, 128, 162, 200셀 처리 중 용적이 가장 큰 50셀에서 초장, 엽 수, 근수, 그리고 근장의 생육이 우수하였다. 그러나 생산비용 과 플러그 육묘의 효율성을 고려하여 105셀 이상의 플러그 트레이 중에서 선택하여 육묘하는 것이 효과적이라 판단된다. 차광률에 따른 유묘는 0, 30, 60, 90% 처리 중 30~60% 차 광처리에서 초장, 근수, 그리고 근장이 유의적으로 높게 측정 되어 생육이 양호하였다. 시비처리에서 생중량과 건중량을 제 외한 생육지표를 검토했을 때, 강부추의 적정 시비처리는 속 효성 고형비료(DO-PRO) 0.1g, 갯부추는 속효성 액체비료 (Peters) 주 1회 8mL 엽면시비처리였고 두 종 모두 속효성 시비처리가 효과적이었다. 강부추와 갯부추의 초기 생육에는 30~60% 차광처리가 된 재배플롯에서 원예상토가 충진된 128셀 플러그 트레이에 종자를 파종한후, DO-PRO 0.1g 또 는 Peters 8mL를 주 1회 엽면시비하면서 재배하는 것이 효 과적이라 판단된다.

Iris laevigata, which belongs to the Iridaceae, is now designated as an “endangered” (EN) grade by Korea Forest Service because it does not have many natural sites known for its reckless development and damage to its natural habitats. This study was carried out to establish the propagation protocol from seed to restore the native habitat of the I. laevigata and to utilize it for ornamental purposes. Basically, the appearance and internal structure of seeds were observed and imbibition experiments were conducted. Germination rate was measured by cold stratification experiment, after warm followed by cold stratification experiment, and GA3 treatment experiment. The seeds had underdeveloped embryos, which had grown to about 25% of those of fully matured seed before germination. In the controlled laboratory experiment, after cold stratification at 5°C for 0, 4, 8, or 12 weeks, the seeds germinated to 0, 11.7, 43.4, or 51.7%, respectively, after 4 weeks of incubation at 25°C. After warm stratification (25°C, 8 weeks) followed by cold stratification for 0, 4, 8, or 12 weeks, the seeds germinated to 0, 51.7, 85.0, or 88.3%, respectively, after 4 weeks of incubation at 25°C. GA3 treatment did not overcome the dormancy. Our study determined the dormancy type of I. laevigata seed. Imbibition experiments showed that there was no physical dormancy, and it was also found that there was an underdeveloped embryo when it was observed that the embryo was growing according to the period of incubation. A nd t he e m bryo grew a t relatively w arm temperatures. It is concluded that the seeds of I. laevigata have morphophysiological dormancy (deep simple MPD). This is the first report to determine the dormancy type in seeds of this valuable ornamental plant.

Mitochondrial genomes of three specimens of Gadus chalcogrammus Pallas 1,814 from Korea and Japan were completely analyzed by the primer walking method. They were 16,570~16,571 bp in length, each comprising 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. Their gene orders were identical to those of conspecific specimens, but exhibited unique haplotypes. In the phylogenetic tree, the juvenile Korean and adult Japanese specimens were separated from the dominant clade composed of specimens from Japan, Korea, the Bering Sea, and the Arctic, including the adult Korean specimen.

약용작물인 천궁류를 가해하는 총채벌레류의 발생종을 확인하고자 2018년 7월과 8월 2회에 걸쳐 영주와 봉화의 일천궁과 토천궁 재배지에서 채집하였다. 두 재배지에서 총 5종의 총채벌레류가 발생함을 확인하였고 꽃노랑총채벌레 > 대만꽃노랑총채벌레 > 미나리총채벌레 > 볼록총채벌레 > 대관령총채벌레 > 파총채벌레 순으로 동일하였지만 포획비율에서는 약간의 차이를 보여 주었다. 이러한 결과는 약용작물로 재배하고 있는 2종의 천궁에서 우점종이 꽃노랑총채벌레와 대만총채벌레임을 확인시켜 주었다. 또한 미나리총채벌레와 대관령총채벌레가 천궁류에서 서식하고 있음을 처음 확인하였다.

Germplasm collection of lily in the mountainousarea is laborious and time consuming accompanied withhigh costs and high risk due to inappropriate environmentalconditions. Cryopreservation being an ideal method for thelong-term preservation can be employed in conservation ofvaluable lily germplasm. Previously, we developed cryo-preservation protocol for Lilium germplasm using ‘dropletvitrification’. In this paper, we have chosen shoot tips as amaterial for cryopreservation because of their genetic safetyupon regrowth in tissue culture. Using this protocol, wehave preserved approximately 160 accessions of lily germ-plasm in 2010~2012. The regeneration rates are rangedfrom 54.3% to 58.5% while the survival rates were from58.3% to 66.4%. Among Lilium germplasm cryopreserved,there are some Korean, Chinese, and Taiwanese seedstocks which have good qualities for inter-species hybrid.Moreover, we also conserved Korean wild endangered seedstock, especially Lilium hansonii. The morphological studyof Lilium germplasm regenerated from cryopreserved mate-rial confirmed the stability of clonal material following cryo-preservation. We anticipate this cryo-collection will beavailable and useful to curators or breeders of Lilium andthis cryobank will also facilitate the conservation and inter-national exchange of Lilium germplasm.

국제해사기구 MO에서 추진하는 e-Navigation 전략은 육상 사용자와 해상 사용자에 다양한 해사안전 정보를 제공하기 위한 네트워크와 정보표준이 필요하다고 정의한 바 있으며 대용량, 다종류의 해양공간정보 서비스가 제공될 것으로 예상된다. 그러나 현재 개발 중인 선박과 선박, 선박과 육상 간 통신을 지원하는 선박용 이동통신 기술은 대용량의 공간정보를 전송하기에는 제약이 따르므로, 대용량의 정보를 최적화하고 간소화하는 기법 개발이 요구되었다.

지하 방사성 폐기물 저장소의 오핸기간동안의 거동은 지반의 파괴와 변형에 영향과 암염의 비선형변형의 예 측은 어려운 실정이다. 따라서 본 연구는 암염의 비선형파괴 메커니즘과 비선형 연속체거동의 유한요소모 델 을 개발하였다.

Soybean is a crop of importance economically and nutritionally in many parts of the world. Thanks to many new genes brought from genomic research, It is possible to introduce various candidate genes through genetic transformation to see the performance of the genes in field. In our lab, soybean transformations have been tried for last 10 years to probe the possibility of traits improvement by transformation of new gene into soybean. For this purpose, three different genes were transformed into Korean soybean variety, Kwangan. First, the gene that controls early flowering of plant was transformed into Kwangan. Second, a candidate gene for soybean mosaic virus (SMV) resistance was transformed to produce transgenic plants. Third, another candidate gene for drought tolerance was transformed. All the transgenic plants from three genes transformation were produced for their gene insertion and their expression using PCR, qRT-PCR. Further analysis including harvesting seeds is currently undertaken.

ORE7 gene incorporated into 3 different promoters including pCKLSL-35S, pCKLSL-TP and pCSENIF was transformed to Korean soybean variety Kwangan using highly efficient soybean transformation system. The gene is known to exhibit a delayed leaf senescence phenotype in Arabidopsis. Fourteen, Fifteen and nine transgenic plants were produced from pCKLSL-35S::ORE7, pCKLSL-TP::ORE7 and pCSENIF::ORE7, respectively. Moreover, transgenic plants were confirmed for gene introduction and their expression using PCR, qRT-PCR and RT-PCR. To identify the transgene insertion events, the analysis of flanking sequence was determined. As a results, T-DNA was integrated intergenically in transgenic line 1 of pCKLSL-35S::ORE7 and line 1 of pCSENIF::ORE7. Currently, flanking sequence analysis with pCKLSL-35S::ORE7, pCKLSL-TP::ORE7 and pCSENIF::ORE7 is carrying out to investigate the stable T-DNA insertions.

e-Navigation strategy IMO promotes is defined as it is necessary for network and information standard to provide various maritime safety informations to in land and on board users, and it is expected to provide a large amount and diverse kinds of maritime spatial information services to them. However, as there are some limits to transmit that by current mobile maritime communication technologies, it is required to simplify and optimize the information. In this study, tree node method is applied to ENC using Douglas-Peucker algorithm and we arranged the effect of simplified spatial information through CALS method which compensates the defect of basic tree node method.

Climatological trend for the period of 1970 to 2009 in sea water temperature around the Antarctic Peninsular waters in the Southern Ocean was investigated. During the period from 1970 to 2009, sea water temperature in the top 500 m water column except 100 m increased at a rate of 0.003 - 0.011℃·yr-1, but at 100 m it decreased at a rate of -0.003℃·yr-1. Although long-term trend is generally warming, there were several periods of sharp changes between 1970 and 2009. Annual mean sea water temperature between surface and 500 m except 100 m decreased from the early of 1970s to the end of 1980s, and then it increased to the end of 2000s. In the entire water column between the surface and 500 m, sea water temperature closely correlated with the El Nino events expressed as the Southern Oscillation Index(SOI), and SOI and sea water temperature have a dominant period of about 3-5 years and decade.