Mycelial growth of Oyster mushroom is very important. Because the management of careless mycelial growth can be caused contamination and low yield of fruit body. To reduce contamination, it is necessary to maintain clean cooling room, inoculation room and cultivation room. First of all, we investigate death rate of microorganism according to ultraviolet(uv) irradiation in cooling room, inoculation room and contamination rate according to sterilization condition of substrate. Though contamination rates according to sterilization conditions were not showed big differences, the amount of electricity-used was the lowest at 121°C for 90 minute. As results of UV irradiation time effect on death rate of microorganism, density of bacteria was not detected after UV irradiation for 6 hours by using UV lamp(40watt) in the room of 56m3. However the death rate of fungi is not big in the same UV irradiation conditions.

In previous studies, we selected green LED(light emitting diodes) for suitable wavelength of light by higher commercial yields and lower ratio of abnormal fruit body in Lentinula edodes. In this study, we aim to select efficient irradiation intensity of green LED. Stronger irradiation intensity of green LED resulted in more polyphenol contents of fruit body. In four level of irradiation intensity, 5, 10, 15, 20umol/m2/sec there was no big difference in commercial yields and characteristics of fruit body. In addition, Lower irradiation intensity resulted in smaller amount of electircity used. In conclusion, we selected 5umol/m2/sec for suitable irradiation intensity in Lentinula edodes with sawdust substrate cultivation.

Recently, Hypsizigus marmoreus is noticed as new mushroom item in Korea. However, this mushroom has the long incubation period about 80 days including pre-incubation period. This study was attempted to establish the optimal post-incubation temperature and period of Hypsizigus marmoreus for bottle cultivation. The most effective post-incubation temperature and period were 25℃ and 20days, respectively. The higher yield was obtained under 25℃ for 20 day after 30 days of pre-incubation. Therefore, total cultivation period was shortened from 106 days (control) to 78days. It is useful information to increase rotation time of cultivation for effective management in H. marmoreus.

We found about cultivation environment conditions of N. lepideus. For the high quality of N. lepideus, lights-out was useful. In the lights-out, the diameter of pileus of N. lepideus was smaller, and the length and the thickness of stipes were longer and larger than in that of lighting. For the efficient cultivation of N. lepideus, temperature of mycelial growth and development of the fruit body were suitable 20～23℃ and 20℃, respectively. In addition, we found higher yield and better quality of N. lepideus at 1,500～2,000ppm of CO2 concentration during the development of fruit body.

느타리버섯 병 재배용으로 널리 사용되고 있는 미송톱밥, 비트펄프, 면실박(50:30:20) 혼합배지에 춘추느타리 2호를 재배한 배지를 풀버섯 재배 배지인 면실피펠릿, 밀기울, 탄산칼슘분말(90:9:1)배지에 혼합하여 상자재배시험을 통한 배양 및 생육특성을 비교분석한 결과, 느타리버섯 수확 후 배지의 첨가량이 많을수록 배지의 pH는 낮아지는 경향이었고, 총질소 함량이 높아 C/N율이 낮았다. 또한 미생물 밀도는 느타리버섯 수확 후 배지 첨가량 50%일 때 가장 높았으며 오염은 첨가량 0, 25, 50%가 10~13%로 비교적 낮은 오염율을 보였다. 균사배양기간 7일, 초발이 소요일수는 11~14일, 수확기간은 3~5일로 처리 간의 차이가 적어 총재배기간도 큰 차이를 보이지 않았다. 수량은 기본배지인 면실피 펠릿 발효 배지(대조구, 356g/상자)보다 수확 후 배지 첨가량 50%+기본배지(면실피 펠릿 발효 배지)처리구가 같은 수량을 나타냈으며, 또한 수확 개체수 및 개체중도 대조구와 동등하여 수확 후 배지를 이용한 배지재료 절감효과가 인정되었다.

This study was carried to investigate the suitable conditions of Pleurotus eryngii through precooling for the more long-term freshness. The time to be same temperatures between P. eryngii and storage room through precooling at 0℃ and 4℃ were showed 2 hours and 5.5 hours, respectively. P. eryngii was cooled within 6 hours and 18 hours at 0℃ and 4℃ with two type, forced air cooling and pressure cooling. After precooling, P. eryngii was packed 400±10g with anti-fog film, and then stored at 4±1℃. In all treatment of precooling, the weight loss was peaked at 25 days and hardness of P. eryngii were decreased at 10 days during storage. There were no significant freshness differences between precooling type and conditions. It was found that the optimum stored period of P. eryngii at 4℃ after precooling was estimated to be 30~35days. Consequently, it is necessary to elucidate efficient and economic precooling conditions.