본 연구는 맹종죽(Phyllostachys edulis) 부산물의 고부가가치 활용을 위한 기초연구로, 맹종죽림 조사 및 소각처리를 통한 죽회 생산 기술을 개발하고자 하였다. 맹종죽림 현장에서의 죽간 벌채 후 남은 상층 부산물(잎, 잔가지, 끝대)과 수년간 방치된 하층 부산물의 바이오매스 총량을 파악한 결과, 입죽 상태의 맹종죽을 벌채할 경우, 활용될 죽간의 중량비는 56.6%, 죽림 내에 방치되는 상층 부산물의 중량비는 43.3%로 나타났다. 방치됐던 하층 부산물은 ha당 약 26톤이 잔존해 있었다. 한편, 부산물을 죽회로 활용하기 위해 소형 소각로 및 대형 무연소각로를 이용한 소각처리를 수행하였다. 현장에 설치된 소각로에서의 조사 결과, 소형소각로(화실, 57.2 ㎥)는 1시간에 10 kg, 1일 최대 80 kg을 소각하는 반면, 대형 무연소각로 (화실, 791.4 ㎥)는 1시간에 70 kg, 1일 최대 560 kg을 소각하여 약 7배의 효율을 보였다. 2차 회화 과정을 통해 얻은 순수 죽회의 수율은 약 84%였으며, 칼륨(80,000 ppm↑), 칼슘(50,000 ppm↑), 인(20,000 ppm↑), 마그네슘(20,000 ppm↑), 망간(10,000 ppm↑), 철(10,000 ppm↑) 등 다양한 무기원소가 함유되어 있었다. 본 연구는 대나무 벌채 현장에서 수행된 실험을 바탕으로, 맹종죽림의 지속 가능한 경영과 새로운 부산물 활용 방안에 대한 제시로 큰 의의가 있을 것으로 판단된다.

Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.

Background : Cynanchum wilfordii and Cynanchum auriculatum belong to the Asclepiadaceae family and appear morphologically similar. In order to discriminate them, it is needed to find the presence of sap and the leaf shapes: C. auriculatum has a blade ovate leaf comparing to C. wilfordii. However, in the herbal medicine market, they have been handled as cut and dried roots. Due to their similar morphology, it is limited to distinguish the roots of C. wilfordii and C. auriculatum. Recently in Korea, it has been a critical issue to misuse these two roots in the herbal market and food industry. Thus, it is required to establish a robust tool for the discrimination and quality control of them. Methods and Results : To separation and characterization of flavor compounds, C. wilfordii and C. auriculatum samples were analyzed by head space solid phase micro extraction (HSS) fiber coupled with gas chromatography-mass spectrometry using Rtx-5MS (30 m x 0.25 mm x 0.25 μm) column. As a result, We have identified compounds of a few hundred in aliphatic aldehydes and aliphatic alcohols, alkenes and acids, aromatic compounds, aromatic compounds containing nitrogen & sulfur, etc,. In particular, The aliphatic and aromatic compounds had been clearly separated on the second dimensional direction by using two-dimensional GC. Conclusion : The volatile flavor compounds of C. wilfordii and C. auriculatum could easily analyzed without pre-treatment with improved resolution and sensitivity using HSS-GCxGC-TOFMS. We have identified compounds of a few hundred in C. wilfordii and C. auriculatum sample. And It was more accurately qualitative confirmed with separation of GCxGC and TSD. We have confirmed the PCA and PLS-DA Plot that was classified depending on C. wilfordii and C. auriculatum through multivariate statistical analysis of the identified flavor compounds.