본 조사지인 돌리네 습지보호지역은 경북 문경시 산북면 굴봉산 정상부에 위치하였으며, 물이 고이기 힘든 돌리네(doline) 지형에 형성된 희귀 습지이다. 뿐만 아니라 멸종위기종과 법적보호종이 분포하는 등 생물다양성 또한 매우 풍부한 것으로 평가되고 있다. 그러나 습지보호지역 주변으로 과수원 등 경작지가 형성되어 있어, 관리 및 보전을 위한 조사 및 대책이 필요하다. 따라서 본 조사에서는 습지보호지역의 체계적인 관리를 위하여 돌리네 습지의 곤충상을 조사하였다. 조사 방법에는 곤충의 다양한 습성과 서식환경을 고려하여, Searching, Sweeping, Pitfall trap, Bucket trap으로 총 4가지의 채집방법으로 조사를 실시하였다. 그 결과 총 11목 82과 188속 237종이 확인되었으며, 이중 딱정벌레목이 가장 많이 출현하였다. 특이사항으로 환경부지정종 51종(중복포함)이 조사되었으며, 그중 멸종위 기종 1종이 확인되었다. 본 연구를 통해 돌리네 습지보호지역의 곤충상을 파악하고 관리 및 보전에 대한 기초자료를 제공하고자 한다.

Ohmic heating is one of advanced thermal processing techniques which utilize conversion of electrical energy into heat. In our study, a feasibility of ohmic heating was tested to cook instant rice cake to improve energy efficacy as an alternative heating methods of conventional electrical kettle. Ohmic heating was conducted using customized ohmic cell (7.5×4.5×9.5 cm) equipped with titanium electrodes. Instant rice cakes in soup were ohmically heated up to 100°C at different electric fields (9, 12, 15 & 18 V/cm) and temperature holding times (60, 80, 100 & 120 s). Thermocouple was placed into both soup and rice cake to evaluate the temperature profile and energy efficacy. Temperature, voltage and current across the sample were measured and recorded at every 3 s using data acquisition system (DAQ). Mathematical model was developed to calculate the internal energy generation rate (QR, W). Internal energy generation rate (QR, W) was integrated versus temperature come-up time (s) to compute the total internal energy dose (ET, J) using MATLBA software. For energy efficacy (Eff), it was calculated ratio of total internal energy dose (ET, J) to heat quantity (Qh, J). During ohmic heating, temperature come-up time was significantly reduced as a function of elevated electric field (P<0.05). For example, 9V/cm of electric field showed 6.2±0.4 min of temperature come-up time up to 100°C. Higher electric field at 18 V/cm reduced temperature come-up time to 1.9±0.1 min. The electric field of 15 V/cm showed the best energy efficacy as 0.78 which meant 78% of electrical energy was converted into thermal energy for heating. In the texture profile analysis, the most preferable harness was found as 6.191 N at 15 V/cm and 100 s holding time. Our study showed the potential of ohmic heating to cook instant rice cakes for home meal replacement (HMR) and outdoor foods.

미꾸라지에서의 Nitrofuran계 대사물질인3-amino-2-oxazolidone (AOZ), 5-morpholinomethyl-3-amino-2-oxazolidinone (AMOZ), 1-ammino-hydantoin (AHD)와 semicarbazide (SEM)의 잔류량을 검사하기 위해HPLC-MS/MS를 이용한 신속한 정량법이 개발되었다. 2-nitrobenzaldehyde (2-NBA)를 이용해 50℃에서 1시간 동안 산 가수분해와 유도체화 과정을 거친 뒤에, 액-액 분배로 정제와 추출을 하였다. 회수율은 음성시료에 3가지 농도 0.5, 1.0, 2.0 μg/kg의 표준액을 첨가하여 평가하였고 평균 회수율은 75.1-108.1% 이었다. 정밀성(%RSD)은 일내 8.7% 이하, 일간 8.5% 이하였다. 직선성은 NBAOZ는 0.2-20 μg/Kg, NBAMOZ는 0.8-20 μg/Kg, NBAHD는 0.2-20 μg/Kg, NBSEM 는 0.1-20 μg/Kg 범위에서 모두 상관계수 0.99이상이었다. 검출한계(LOD)는 NBAOZ 0.06 μg/Kg, NBAMOZ 0.24 μg/Kg, NBAHD 0.06 μg/Kg, NBSEM 0.03 μg/Kg이었고, 정량한계(LOQ)는 NBAOZ 0.2 μg/Kg, NBAMOZ 0.8 μg/Kg, NBAHD 0.2 μg/Kg, NBSEM 0.1 μg/Kg 이었다. 가수분해 및 유도체화 소요시간을 1시간으로 줄여 만든 신속 간편한 이 시험법이미꾸라지 중 nitrofuran metabolites잔류량 분석에 적합함을 확인할 수 있었다.

The sexual maturation occurred by the changes of steroid hormones was known to sex-dependently and/or agedependently regulate the lipid metabolism in various animal species. Our current study demonstrates that lipid and its functional fatty acids can be changed depending on the status of sexual maturation. Of the functional fatty acids, γ- linolenic acid (GLA; 18:3n-6) is an important factor for maintaining human health. The purpose of our study was to investigate the level of GLA in mice with different stages of sexual maturation. To this end, the longissimus muscle (LM) of immature (3-week-old) and mature (7-week-old) female mice was analysed for the fatty acid composition by gas chromatography. Furthermore, both gene and protein level of Δ6 desaturase (FADS2) which is involved in GLA metabolism by real time PCR and Western blotting, respectively. Mature females showed greater (P<0.05) serum 17β -estradiol (E2) level and LM GLA contents than immature group. The mRNA and protein levels of FADS2, which converts precursor linoleic acid into GLA, were higher (P<0.05) in mature female mice than in immature mice. In conclusion, these results show that sexual maturation of female mice induces GLA and FADS2 contents in LM.

시간분해 레이저 유도 형광 분광학을 이용하여 UO22+, UO2(OH)+, (UO2)2(OH)22+, (UO2)3(OH)5+와 같은 우라늄(VI) 화학종 규명 연구를 수행하였다. 들뜸 파장의 변화에 따른 화학종 규명 감도를 조사하였다. 266 nm의 들뜸 파장을 이용할 경우, 나노 몰 농도의 U(VI) 화합물을 구분할 수 있는 화학종 규명 감도를 얻었다. 이온 세기가 0.1 M, pH가 1인 조건에서 UO22+ 이온의 형광 스펙트럼과 형광 수명을 측정하였다. 488, 509, 533, 559 nm 파장의 특징적인 형광 봉우리를 관측하였고, 측정한 형광 수명은 1.92±0.17 ㎲ 이었다. U(VI) 가수분해 화합물의 형광 스펙트럼과 형광 수명의 변화를 이 값을 기준으로 비교하였다. 장파장 방향으로 이동한 형광 봉우리와 길어진 형광 수명을 가진 가수분해 화합물의 특징적인 양상을 보고한다.

Making use of our extended version of ¨ O p i k ′ s convection theory, we have calculated magnetic cycle periods of the sun and late type stars by using Parker's dynamo theory, where we have included the non-linear effect. We presented a relationship between the computed cycle period and spectral type to analyze observed magnetic activities of the late type stars and long-term luminosity variations. It is found that (1) the stellar magentic-cycle period increases towards the later spectral type, (2) the rapid rotation facilitates the activity-related luminosity variation of stars later than about K5, (3) differential rotation plays a critical role in determining the magnetic activity-cycle period, and (4) the non-local effect should be taken into account in order to understand the observed long-term luminosity variations.

A generalization of the original ¨ O p i k ′ s cellular convection theory has been made to accomodate a rotating convective medium. With the use of the formulation, a set of rotating model envelopes of the sun and late type main sequence stars have been constructed under three different rotation periods. Their thermal structures are presented and characteristics of their convection are discussed in the context of stellar dynamo. In the present study it is noted that the rotational angular velocity increases in wards with depth, and its increase turns out to be about 6% at the bottom of the solar convection zone.