본 연구는 베트남 하노이 지역에서 8가지 한국 토마토 품종 (핑크탑, 큐피랑, 슈퍼도태랑, 선레드, 선글러브, TP-7 플러스, 러블리 250, 광복)과 1가지 베트남 품종(FM 120)을 대상으로 봄-여름 및 가을-겨울 작기에 따른 수확량 및 과실 특성을 비교 분석하였으며, 그 결과는 다음과 같다. 1. 작기에 따라 초장, 경장, 주당 과실수 및 수확량(kg)은 유의성이 있었으며 가을-겨울 작기에서 높게 나타났다. 전체적으로 한국품종이 베트남 품종에 비해 높은 생육특성을 보인 반면, 과실수와 수확량에서는 베트남 품종이 우수하였다. 2. 토마토 과실의 주요 특성인 과장, 과폭 및 과중은 작기에 따라 유의한 차이를 보였으며 가을-겨울 작기에서 높은 수치를 나타내었지만 TSS 함량은 봄-여름 작기에서 높았다. 건물중과 비타민C 함량은 작기에 따라 유의한 차이가 없었다. 품종별 과실 특성은 한국 품종이 우수하였지만 비타민C 함량은 베트남 품종이 높았다. 3. 봄-여름 작기에서 한국품종의 경우 수확량 및 과실특성에서 가을-겨울 작기 재배에 비해 큰 감소를 보인 반면 베트남 품종은 큰 차이를 보이지 않았다. 따라서 본 실험에 이용된 8가지 한국 품종의 경우 봄-여름 작기보다는 가을-겨울 작기 재배가 적합할 것으로 판단된다.

Antioxidants partially ameliorated the detrimental effects of reactive oxygen species (ROS) on sperm characteristics during in vitro storage. The objective of the present study was to investigate the single or synergetic antioxidative effect of curcumin and Vit. E on the characteristics of fresh boar sperm during in vitro storage. The sperm viability in curcumin, Vit. E supplementation and curcumin+Vit. E+H2O2 groups remained over 85.0% in 3 hr incubation period, but in 6 hr incubation period, curcumin+Vit. E+H2O2 groups was sharply dropped than those of curcumin and Vit. E group. The membrane intergrity in all evaluated groups except for H2O2 group did not significantly difference in 3 hr incubation period. The viability in curcumin or Vit. E supplementation were significantly increased than in curcumin+H2O2 and Vit. E+H2O2 group in 6 hr incubation period. The percentage of mitochondrial activity and acrosome intergrity obtained similar trends within same incubation periods irrespective of treatment. The lipid peroxidation of spermatozoal plasma membrane ranged from 11.6∼17.5 nM/l×106 and 14.0∼ 19.0 nM/l×106 in 3 hr and 6 hr incubation periods. In conclusion, curcumin or Vit. E rpplementation alone or cooperatively improved sperm viability index (motility, membrane intergrity, viability and survival rates) and fertility index (mitochondria activity, acrosome intergrity and lipid peroxidation) of fresh boar sperm, indicating that curcumin and Vit. E have a antioxidative properties through its scavenging activity against hydrogen peroxide.

The aim of this study was to evaluate the effects of different straw volume (0.5 and 5.0 ㎖) on cryopreservation in boar semen. Semen quality was evaluated the motility, viability, abnormality, acrosome intactness and membrane integrity. And there were also examined the development rates of IVM/IVF embryos using frozen-thawed boar semen. Boar semen were frozen until 5℃ for 3 hours using cell freezer and making the straws, and then freezing by lowing the straws into styrofoam box on the 8 cm above the LN2. In different straw volume (0.5 and 5.0 ㎖), sperm viability and abnormality were not differ in 0.5 and 5.0 ㎖ straws, but sperm motility were significantly higher in 0.5 ㎖ straws (61.3%) than in 5.0 ㎖ straws (56.3%) (p<0.05). In the Coomassie Brillient Blue (CBB), Hoechst 33258/Propidium Iodide (H258/PI) staining and Hypoosmotic Swelling Test (HOST), the acrosome intactness and sperm membrane integrity were not differ in 0.5 and 5.0 ㎖ straws, but sperm survival rate was significantly higher in 0.5 ㎖ straw (65.0%) than in 5.0 ㎖l straw (55.0%) (p<0.05). Employing the Chlortetracycline (CTC)/Hoechst33258 (H258), all treatment group were not differ in characteristic of uncapacitated acrosome-intact sperm (F), capacitated and acrosome-intact sperm (B-type) and acrosomereaction seprm (AR-type). In the developmental rate of IVM/IVF embryos using frozen-thawed boar semen in different straw volumes, the developmental rate of morula plus blastocysts were 19.9% in 0.5 ㎖ straw and 18.8% in 5.0 ㎖ straw, respectively. These results indicated that straw volume affects the semen motility and sperm survival rate, but not other semen characteristic and developmental rate of IVM/IVF embryos.