아스타잔틴을 포함하는 수중유형 형태의 나노에멀젼을 고압균질기를 이용하여 제조하였다. 이에 유화조건, 유화제 종류, 유화제 농도 그리고 아스타잔틴 농도에 따라 최적화를 되었다. 나노에멀젼의 안정성은 제타포텐셜, FF-SEM, 입도분석기, 색차계를 이용하여 측정하였다. 제조된 아스타잔틴 나노에멀젼의 입도는 160 ~190 nm로 균일하였으며 레시친에 의한 나노에멀젼 보다 glyceryl citrate/lactate/linoleate/oleate에 의한 나노에멀젼이 더욱 안정하고 균일한 입도분포를 가졌다. 아스타잔틴의 봉입도는 HPLC, FF-SEM을 이용하여 확인 하였으며, 제형 구성 후 보관조건에서의 안정도 및 제타 포텐셜 값도 -41의 우수한 결과를 나타내었다.

To find proper water quality management strategy for oxygen consumption organic matters in Jinhae bay, the physical process and net supply/decomposition in terms of COD was estimated by three-dimensional eco-hydrodynamic modeling. The estimation results of physical process in terms of COD showed that transportation of COD was dominant in loading area from land to sea, while accumulation of COD was dominant in middle～bottom level. In case of surface level, the net supply rate of COD was 0～60 mg/m2/day. The net decomposition rate of COD was 0～-0.05 mg/m2/day(-5～-10 m, in depth) to 2 level, and -0.05～-0.20 mg/m2/day(10 m ～) to bottom level. These results indicate that the biological decomposition and physical accumulation of COD are occurred for the most part of Jinhae Bay bottom. The variation of net supply or net decomposition rate of COD as reducing land based input loading is also remarkable. Therefore, it is important to consider both allochthonous and autochthonous oxygen demanding organic matters to improve the water quality of Jinhae Bay.

The three-dimensional eco-hydrodynamic model was applied to estimate the autochthonous COD caused by production of phytoplankton in Jinhae Bay. A residual current was simulated, using a hydrodynamic model, to have a sightly complicated pattern in the inner part of the bay, ranging from 0.001 to 5 cm/s. In the outer part of the bay, the simulated current flowed out to the south sea with a southward flow at a maximum of 25 cm/s. The results of the ecological model simulation of COD levels showed high concentrations, exceeding 4 mg/L, in the inner bay of Masan, an area of wastewater discharge, and lower levels, approaching less than 1 mg/L, closer to the outer part of the bay. The simulation results of Autochthonous COD by two methods using ecological modeling, showed high ratio over 70% of total COD. Therefore, it is more important to consider nutrients than organic matters in the region for control COD standard.

It is very important to interprete and simulate the variation of phytoplankton maximum region for the prediction and control of red tide. This study was composed of two parts, first, the hydrodynamic simulation such as residual current and salinity diffusion, and second, the ecological simulation such as phytoplankton distribution according to freshwater discharge and pollutant loads. Without the Nakdong river discharge, residual current was stagnated in inner side of this estuary, and surface distribution of salinity was over 25psu. On the contrary, with summer mean discharge, freshwater stretched very far outward and some waters flowed into Chinhae Bay through the Kadok channel, and low salinity extended over coastal sea and salinity front occurred. From the result of contributed physical process to phytoplankton biomass, the accumulation was occurred at the west part of this estuary and the Kadok channel with the Nakdong river discharge. When more increased input discharge, the accumulation band was transported to outer side of this estuary. The frequently outbreak of red tide in this area is caused by accumulation of physical processes. The phytoplankton maximum region located inner side of this estuary without the Nakdong river discharge and with mean discharge of winter, but it was moved to outer side when mean discharge of the Nakdong river was increased. The variation of input concentration from the land loads was not largely influenced on phytoplankton biomass and location of maximum region. When discharge was increased, phytoplankton maximum region was transferred to inner side of the Kadok channel. On the other hand, when discharge was decreased, phytoplankton maximum region was transferred to inner side of this estuary and chlorophyll a contents increased to over 20㎍/L. Therefore, if any other conditions are favorable for growth of phytoplankton, decrease of discharge causes to increase of possibility of red tide outbreak.