To enhance the embryo preservation technology and better application of embryo transfer technique to the field (dairy science or animal reproduction. etc.), we examined the viabilities of bovine embryos produced in vitro and in vivo after cryopreservation according to their developmental stage and thawing temperature. Bovine embryos from in vivo/vitro fertilization (Hanwoo) were examined at day 7, 8, and 9. Survival rates and total cell numbers of in vivo fertilized embryos were as follows: morulae 68.8% and ; blastocysts 80.5% and ; expanded blastocysts 77.4% and , respectively. Rates of embryo development for blastocysts and expanded blastocysts after thawing were significantly higher than that of morula stage embryos (p<0.05). While survival rates of in vitro fertilized embryos according to developmental stage showed no significant difference among groups (morula 67.9%; blastocyst 74.3%; and expanded blastocyst 79.4%), total cell numbers were significantly lower than those of other groups (morula ; blastocyst ; and expanded blastocyst ) For the viability according to thawing temperature, survival rate was higher in .

Rice is a staple food for more than 50% of the world's population. Embryo comprises only 2 to 3% of the weight distribution of the entire pericarp but has higher concentration of vitamins, proteins, and essential fatty acids than the other parts of grains. Moreover, α-tocoperol, γ-oryzanol, phytic acid and γ-aminobutric acid that have nutraceutical value are abundant. Increasing the volume of embryo assures the fortification of nutritional value of rice grain. We developed new black waxy giant embryo rice, Milyang 263 by crossing Josaengheugchal, a black waxy rice variety, and get, a giant embryo mutant generated by tissue culture. The nutrient contents and physical properties of Milyang 263 were compared with several giant embryo mutants and normal embryo rice varieties. Changes in the nutrient properties after germination were also observed. Results indicated that this new black waxy giant embryo rice, Milyang 263, offers a promising source for improving nutritional quality of rice especially anthocyanin, essential minerals, and GABA.

To find out the best complex fertilizer for high yielding of rapeseed crop, experiment was conducted on complex fertilizers at the experiment field in upland of rapeseed in Mokpo Experiment Station, Nat'l Institute of Corp Science, RDA, Korea. Experiment was laid out in randomized complete block (RCBD) design. The effects of complex fertilizer (22-22-11) on the number of branches, pod length, percentage of seed set and seed yield were highest but on the plant height, ear length, and number of pods per ear were negligible. On th basis of the results reported above, for getting higher yield of rapeseed crop, among the tested fertilizers complex fertilizer (22-22-11) gave the superior performance and is recommended for application.

The effect of plant growth regulators was investigated on in vitro shoot proliferation from axillary bud explants of Hypericum erectum. To determine the optimal cytokinin for proliferation of axillay buds, we carried out screening four cytokinins (BA, kinetin, 2iP, TDZ). When nodal segments were cultured on MS medium supplemented with 4.5 μM TDZ (thidiazuron), a number of shoots were induced. Our results indicated that the addition of TDZ to culture medium resulted in the induction of significantly more axillary buds than in the addition of other cytokinins. The optimal concentration of TDZ for proliferation of axillary buds was 10 μM. 92% of shoots spontaneously rooted without any plant growth regulator (PGR) and formed whole plantlets within one month. More than 95% of these regenerants survived and they did not show any detectable variation in morphology or growth characteristics compared to their donor plants.

In order to select the seeding machine for mechanizing cultivation of rapeseed in southern areas of Korea, three different seeding machines, ridge rotary, power tiller ridge rotary, tractor ridge rotary were used for sowing one of the high yielding rapeseed cv. Hybrid with five different seeding methods. Seeding of ridge rotary was reduced the seeding effort with 45% and yield components such as plant height, ear length, number of branches and pods, pod length and seed setting rate were higher. The seeding of ridge rotary also was showed highest seed yield. On the basis of time requirement for seeding, vegetative and yield parameters ridge rotary seeding machine was a suitable seeding machine for rapeseed cultivation at the southern area of Korea.

The objectives of this experiment was to evaluate the effect of seeding and transplanting date on yield and quality of rapeseed experiment were conducted in the research field of Chungcheongnam Do(Taejun) Agricultural Research and Extension Services with Yudal, one of the rapeseed cultivar of different growth characteristics among the six treatments were examined and recorded. Results showed that yield components such as plant height, length of ear and pod, number of branches and pods and percentage of pod setting and seed set were highest at the plot with direct seeding in Sep. 10. In this treatment yield of seed, 1,000 grains weight and weight of 1l were highest. On the basis of vegetative and yield parameter of direct seeding in Sep. 10 was the best sowing date of rapeseed crop in Chungcheongnam Do region of Korea. Experiments were conducted in the research field of Chungcheongnam Do(Taejun) Agricultural Research and Extension Services with Yudal variety, which showed different growth characteristics. Yield components such as plant height, length of ear and pod, number of branches and pods and percentage of pod setting and seed set were highest at the plot with direct seeding in Sep. 10. Yield of seed, 1,000 grains weight and weight of 1l were highest at the direct seeding in Sep. 10. Judging from the results reported above, seemed to be direct seeding in Sep. 10.

This study was conducted to evaluate the control effect of fungicides of sclerotinia rot control, growth characteristics, and seed yield in the cultivation of rapeseed. All fungicides treated had no effect on the growth of rapeseed. The major fungicides were mancozeb 75% WP, chlorothalonil 75% WP, dithianon 43% WP. difenoconazole 10% WP, benomyl 50% WP, and propineb 70% WP. Dry seed yield and control were increased largely with chlorothalonil 75% WP (33g/12l), fungicide than the other fungicides Sclerotinia rot. All fungicides had no injury with standard dosage. On the other hand all fungicides had slight injury in the double dosage level for the rapeseed.

Low temperature is one of the most severe abiotic stress factors limiting growth, productivity and distribution of winter cereals. Reliable field screening method, which can detect small differences in winter survival, is important for the effective selection and development of plants to identity superior cold tolerant winter cereal genotypes. This study was undertaken to provide improved screening method of winter hardiness in the field by increasing the accuracy in evaluating winter hardiness of barley (Hordeum vulgare L.). We introduced furrow in field screening of winter survival. By sowing the plants at the ridge and base, we could minimize the effect of topographic variation in a field by giving higher and lower level of stress at the same time. This method could be used by breeders to conduct accurate evaluation of winter hardiness by selecting the better treatment, which shows close to normal distribution, among the winter survival rate from the ridge, base and mean survival rate of the two in a screening field.