Owing to the great demand for portable and wearable chemical sensors, the development of all-solid-state potentiometric ion sensors is highly desirable considering their simplicity and stability. However, most ion sensors are challenged by the penetration of water and gas molecules into ion-selective membranes, causing unstable and undesirable sensing performances. In this study, a hydrophobic ionic liquid-modified graphene (Gr) sheet was prepared using a fluid dynamics-induced exfoliation and functionalization process. The high hydrophobicity and electrical double-layer capacitance of Gr make it a potential solid-state ion-to-electron transducer for the development of potentiometric sodium-ion ( Na+) sensors. The as-prepared Na+ sensors effectively prevented the formation of the water layer and penetration of gas species, resulting in stable and high sensing performances. The Na+ sensors showed a Nernstian sensitivity of 58.11 mV/[Na+] with a low relative standard deviation (0.46), fast response time (5.1 s), good selectivity (K < 10− 4), and good durability. Furthermore, the Na+ sensor demonstrated its feasibility in practical applications by measuring accurate and reliable ion concentrations of artificial human sweat and tear samples, comparable to a commercial ion meter.

A facile and efficient method was developed to prepare highly stretchable and conductive graphene conductors with wrinkled structures by the mechanical stretching and shrinking of elastomeric substrates, in which graphene inks were printed on a prestretched elastomeric substrate. Stretchable and exfoliated graphene inks were prepared by mixing graphite and Ecoflex in a shear-assisted fluid dynamics reactor. The resultant graphene conductor exhibited excellent stretchability at 150% strain and high electrical conductivity of 64 ± 1.2 S m− 1. The resistance of the conductor did not change in bent, twisted, and stretched states. The resistance did not change during 10,000 cycles of stretching/releasing, with a maximum strain of 150%. Based on the graphene conductor, a stretchable conductometric sensor with a two-electrode configuration was fabricated to measure impedance changes at different concentrations of electrolyte ions. This sensor exhibited a good and linear sensitivity curve (298.61 Ω mM− 1, R2 = 0.999) in bent and stretched states.

This study was conducted at the National Institute of Animal Science (NIAS) from 2010 to 2017 to develop a late-maturing variety with high productivity for cultivation in the southern region of South Korea. The new variety of Italian ryegrass, ‘IR901’, was a late-maturing variety, and its heading date was 22 May, 17 days later than that of the control variety ‘Kowinearly’. ‘IR901’ had a flag leaf width of 11.2 mm, flag leaf length of 31.8 cm, and plant length of 103 cm on its heading date. The combined average dry matter yield of ‘IR901’ in all three adaptability evaluation regions (Cheonan, Pyeongchang and Haenam) was 7,747 kg/ha, which was similar to that of the ‘Kowinearly’ variety (7,734 kg/ ha). However, the average dry matter yield over three years in Cheonan and Pyeongchang was 82% and 96%, respectively, compared to that of the control, which was most likely because of the poor cold tolerance of ‘IR901’. By contrast, in Haenam, in the southern region, the average dry matter yield of ‘IR901’ was 19% higher than that of the ‘Kowinearly’ variety. The proportions of crude protein (CP), total digestible nutrients (TDN), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in ‘IR901’ were 8.6%, 59.7%, 36.9%, and 54.8%, respectively; the proportions were 0.2% lower, the same, the same, and 2.5% lower than those in the ‘Kowinearly’ variety. The determined in vitro dry matter digestibility (IVDMD) of ‘IR901’ was 72.2% higher than that of ‘Kowinearly’ (67.2). In general, of the two varieties, the forage quality of ‘IR901’ was marginally superior to that of ‘Kowinearly’.

In weapon assignment studies to defend against threats such as ballistic missiles and long range artillery, threat assessment was partially lacking in analysis of various threat attributes, and considering the threat characteristics of warheads, which are difficult to judge in the early flight stages, it is very important to apply more reliable optimal solutions than approximate solution using LP model, Meta heuristics Genetic Algorithm, Tabu search and Particle swarm optimization etc. Our studies suggest Generic Rule based threat evaluation and weapon assignment algorithm in the basis of various attributes of threats. First job of studies analyzes information on Various attributes such as the type of target, Flight trajectory and flight time, range and intercept altitude of the intercept system, etc. Second job of studies propose Rule based threat evaluation and weapon assignment algorithm were applied to obtain a more reliable solution by reflection the importance of the interception system. It analyzes ballistic missiles and long-range artillery was assigned to multiple intercept system by real time threat assessment reflecting various threat information. The results of this study are provided reliable solution for Weapon Assignment problem as well as considered to be applicable to establishing a missile and long range artillery defense system.

This study was conducted at the National Institute of Animal Science from 2010 to 2017. As a variety that is sufficiently productive in the southern regions to replace imported varieties and sufficiently cold-resistant to be cultivated in the central-northern regions, "IR605" was developed and submitted to the Korea Seed & Variety Service in an application for protection. The novel Italian ryegrass variety "IR605" is a diploid with green leaves, a semi-erect growth habit before wintering, and an erect growth habit in the spring. "IR605" was a medium maturing variety with a heading date of around May 15th. "IR605" had a flag leaf width of 9.9 mm, flag leaf length of 26.7 cm, and plant length on the heading date of 100 cm, which was approximately 5 cm longer than "Kowinearly." The stem thickness and ear length of "IR605" are 0.08 mm thicker and 0.5 cm longer than those of "Kowinearly", respectively. The cold-resistance of "IR605" was weaker than that of "Kowinearly", but strong enough to be cultivated in Pyeongchang, Gangwon province. The dry matter yield of "IR605" (9,308 kg/hectare) was 20% higher than that of "Kowinearly", which was further pronounced in the southern region of Haenam, where there was a 52% increased (p < 0.05). The in vitro dry matter digestibility of "IR605" was 68.4% at which was slightly higher than that of "Kowinearly", The total digestible nutrients was 58.5%, which was slightly lower than "Kowinearly". Overall, the feed quality characteristics of "IR605" were similar to those of "Kowinearly".

This experiment was carried out to study the effects of different environmental conditions and cultivation techniques on productivity of grasslands in central and southern area of Korea on 2017 and 2018. Average dry matter yield of grasslands at 10 actual production sites was 7,496 kg/ha. that was ranged from 4,652 to 13,292 kg/ha with least significant difference(LSD) of 1,577kg/ha between grasslands (p<0.05) on 2017. Average dry matter yield of grasslands at 10 actual production sites was 7,914 kg/ha. that was ranged from 3,927 to 12,372 kg/ha with LSD of 1,577kg/ha between grasslands(p<0.05) on 2018. Dry matter(DM) yield of grasslands have positive correlation with soil fertility (p<0.01) but not correlated with rainfall and air temperature among cultivation environments. And also DM yield of grasslands have positive correlation with grassland management techniques(p<0.01). These results suggest that practices of grassland management techniques and improvement of soil fertility are more important than cultivation environments by climate change for increasing the DM yield of grassland in central and southern area of Korea.

This study was conducted to find out the effects of seed mixture using new domestic grass varieties orchardgrass ‘Onnuri’ and tall fescue ‘Greenmaster’ on botanical composition and dry matter productivity from 2015 to 2018 in oversown hilly pasture, the central northern area of Korea. This experiment was consisted of 4 mixed grasses (Treatment 1 : tall fescue oriented mixture with imported varieties; Treatment 2 : orchardgrass oriented mixture with imported varieties; Treatment 3 : orchardgrass oriented mixture with domestic varieties, Treatment 4 : tall fescue oriented mixture with domestic varieties). Evaluation of seasonal changes in botanical composition of pasture showed that orchardgrass was the highest in all treatments, ranging from 46 to 89% and Kentucky bluegrass was the second highest in all treatments. Treatment 3 (7,633 kg ha-1) and Treatment 4 (7,570 kg ha-1), mixed grasses using domestic varieties, were showed the highest dry matter yield than Treatment 1(6,950 kg ha-1) and Treatment 2 (6,934 kg ha-1), which were mixed grasses using introduced varieties. Therefore, these results showed that the orchard grass oriented grasses mixture with domestic varieties was good for grassland vegetation and productivity in oversown hilly pasture, the central northern area of Korea.

We report potentiometric performances of ion-to-electron transducer based on reduced graphene oxide (RGO) for application of all-solid-state potassium ion sensors. A large surface area and pore structure of RGO are obtained by a hydrothermal self-assembly of graphene oxide. The extensive electrochemical characterization of RGO solid contact at the interface of ionselective membrane and gold electrode shows that the potassium ion-selective electrode based on RGO had a high sensitivity (53.34 mV/log[K+]), a low detection of limit (− 4.24 log[ K+], 0.06 mM) a good potential stability, and a high resistance to light and gas interferences. The potentiometric K+- sensor device was fabricated by combining of screen-printed electrodes and a printed circuit board. The K+- sensor device accurately measures the ion concentration of real samples of commercial sports drinks, coke and orange juice, and then transfers the collected data to a mobile application through a Bluetooth module. The screen-printed ion sensors based on RGO solid contact show a great potential for real-time monitoring and point-of-care devices in human health care, water-treatment process, and environmental and chemical industries.

This experiment was carried out to study the effects of different environmental conditions and cultivation techniques on productivity of forage corn in central and southern area of Korea on 2017 and 2018. Average dry matter yield of forage corn at 34 cultivation regions was 13,510kg/ha. Forage productivity of forage corn cultivated at actual production sites have positive correlation with cultivation techniques(p<0.01) but not correlated with cultivation environments. Forage productivity of forage corn have positive correlation with seeding techniques(p<0.01) but not correlated with fertilization techniques. These results suggest that practices of cultivation techniques are more important than cultivation environments for increasing the forage productivity of forage corn. Therefore, yield prediction techniques of forage corn in Korea have to be considered the practices of cultivation techniques along with soil and climate conditions.

Salicylic acid (SA) is an essential plant growth regulator that functions as a signaling molecule in plants. The purpose of this study was to clarify how the exogenous application of SA counteracts aluminum stress-induced growth and biomass yield reduction in alfalfa exposed to aluminum (Al) stress. Two-week-old alfalfa seedlings were exposed to a combination of AlCl3 (0 μM, 50 μM and 100 μM, respectively) and SA (0.1 mM) for 72 hours. We observed, Al stress-induced plant growth inhibition and forage yield reduction are Al stress-dependent manner. A significant reduction of plant height (42.0-52.9%), leaf relative water content (13.0-21.4%), root length (35.4-48.7%), shoot fresh weight (31.2-25.9%), root fresh weight (15.4-23.3%), shoot dry weight (12.7-22.2%), roots dry weight (47.3 -53.5%), were observed in alfalfa. In contrast, SA alleviated the Al-stress and enhanced growth and biomass yield in alfalfa. This study provides useful information concerning the role of SA that counteracts aluminum stress-induced growth and yield reduction in alfalfa.

Soil acidity or alkalinity are serious limitations for crop production. The purpose of this study was to clarify the negative effects of extreme pH stress (low and high) on alfalfa vegetative growth (VG) and biomass accumulation (BA). Two-week-old alfalfa seedlings were exposed to different pH (4.0, 4.5, 7.0, 8.0 and 8.5, respectively) levels for 72 hours. Alfalfa grown at pH 4.0 and 8.5 significantly reduced VG and BA, wherein as neutral pH (7.0) comparably exhibited better growth and biomass yield. These results indicate that extreme acidic or alkaline level are critical limiting factors for growth and biomass yield in alfalfa.

This study was conducted to investigate the effect of seeding dates on grassland productivity and botanical composition in oversown pasture located in Cheonan of South Korea. Four treatment groups were established based on the seeding dates: 18th August, 1st September, 15th September and 29th September. Evaluation of seasonal changes in botanical composition of pasture showed that the highest ratios of grass in 18th August and 1st September (pasture species 93% and weeds 7%) and the lowest in 15th September (pasture species 75% and weeds 25%). In the plant length, there is no significantly different in 5% probability level. In the total dry matter yield of grass, 18th August (13,362 kg ha–1) and 1st September (13,988 kg ha–1) were higher than 15th September (11,883 kg ha-1) and 29th September (11,459 kg ha-1). The findings of the this study suggest that seeding by early September the most desirable results for botanical composition and grassland productivity in oversown pasture, Cheonan of South Korea.

This experiment was carried out to evaluate the characteristic and productivity of forage corn varieties sown at the last ten days of May at Cheonan of Korea on 2017 and 2018. Forage corn varieties sown on 24th May 2017 and 25th May 2018 were emerged in 5th and 1st June, respectively. Silking date of forage corns was different among varieties(p<0.05). Stem length of forage corns was not different among the varieties, except Kwangpyeongok(KPA) and Nero IT. Stem diameter of forage corns was not different amaong the varieties. Outbreak rate of black streaked dwarf virus was lower than 3% in all varieties. Total digestible nutrient(TDN) yield was different among corn varieties(p<0.05), which was higher in P32P75, followed by P2088, P31N27, and KPA. The TDN yield of varieties had direct correlation with stem length, stem diameter and ear yield, respectively(p<0.01). Average of TDN in all varieties was 69.8%. These results suggested that forage corn variety with late-seeding adaptability is more important for increasing the productivity of forage corn If harvesting of winter forage crops have to be finished in the middle ten days of May in middle region of Korea.

본 시험은 사료가치가 우수하고 엔도파이트에 감염되지 않은 톨 페스큐 신품종을 개발하기 위하여 2010년부터 2017년까지 농촌진흥청 국립축산과학원 초지사료과에서 수행되었다. 톨 페스큐 신품종 ‘그린마스터4호’는 6배체 품종으로서 잎은 녹색이며, 출수기가 5월 16일경으로서 대비품종인 ‘Fawn’ 보다 5일 늦은 중생품종이고, 출수기에 초장은 74.6cm 정도이다. 지역적응시험 4개 지역평균 건물수량은 ‘그린마스터4호’가 16.2톤/ha로서 대비품종인 ‘Fawn’보다 5% 많았으며, 지역별 생산성이 4개 지역 모두에서 ‘Fawn’보다 우수하였다. ‘그린마스터4호’의 사료가치는 조단백질(CP) 함량이 15.2%, 건물소화율(IVDMD)이 70.0%, 가소화양분총량(TDN)이 63.6%, 중성세제불용섬유(NDF)가 58.7%, 산성세제불용섬유(ADF)는 32.0%로서 Fawn보다 높거나 대등하였다. ‘그린마스터4호’는 엔도파이트에 감염되지 않는 품종이면서 ‘Fawn’보다 국내 기후환경 적응성과 영속성이 우수하여 초지조성에 알맞은 품종으로서 우리나라 전국에서 재배 가능하다.

본 연구는 이탈리안 라이그라스 재배현장에서 재배환경과 재배기술의 차이에 따른 조사료 생산성의 변화 요인을 분석 하기 위하여 2016부터 2017년까지 수행되었다. 우리나라 중부 및 남부지역에서 27개 이탈리안 라이그라스 재배지의 평균 건물수량은 6,940kg/ha이였다. 이탈리안 라이그라스 재배현장의 생산성은 재배환경보다 재배기술과 높은 상관(p<0.01)이 있는 것으로 나타났다. 재배기술 중에서 파종기술 및 포장관리기술과 생산성 간에는 고도의 상관이 있었으나, 시비기술과 생산성 간에는 상관이 없었다. 이상의 결과는 이탈리안 라이그라스의 생산성 향상에는 재배환경보다 재배기술의 현장실천이 더 중요함을 제시하고 있다. 따라서 우리나라 지역별 이탈리안 라이그라스의 생산성 예측기술의 정밀도를 높이기 위해서는 그 지역의 기상 및 토양환경은 물론 재배기술의 적용을 충분히 고려하여야 할 것으로 사료된다.

본 연구는 평창지역의 산지에서 목초 파종시기가 초지의 연차별 식생 및 생산성에 미치는 영향을 구명하고 평창지역의 산지초지 조성에 적합한 피종시기를 설정하고자 2015년 8월부터 2017년 10월까지 평창군에 위치한 서울대학교 국제농업기술대학원의 산지에서 수행되었다. 처리는 총 4처리로 T1: 8월 13일 파종 T2: 8월 27일 파종, T3: 9월 10일 파종, T4: 9월 24일 파종이었다. 월동 후 초지 식생구성을 보면 T1과 T2까지는 목초 식생비율이 98%이상으로 높은 식생밀도를 보였지만 T3이후부터는 급격하게 감소하였다. 분얼의 경우 T1과 T2에서 4.4개 이상으로 양호하였지만 T3이후에는 급격하게 감소하였다. 이러한 초기 월동률의 차이는 이후 목초의 식생 및 생산성에 큰 영향을 주었다. 연차별 식생변화를 보면 T1과 T2의 경우 목초 비율이 81%이상으로 우수하였지만 T4의 경우 상대적으로 불량하였다. 전체 건물 수량의 경우 T2와 T1이 각각 9,042 kg ha-1 와 8,845 kg-1 로 우수하게 나타났지만 T4의 경우 5,086 kg ha-1 로 건물 생산성이 상대적으로 낮게 나타났다. 결과를 종합해보면 평창지역에서 8월 말까지 파종을 완료 한 경우 목초 월동이 향상되었으며 이는 식생이나 생산성 측면에서 유리하게 나타났다.

본 연구는 이탈리안 라이그라스 품종별 내한성 및 생육특성의 변화와 그에 따른 생산성 변화요인을 분석하기 위하여 2017년부터 2018년까지 우리나라 중부지역인 충남 천안에서 수행되었다. 이탈리안 라이그라스의 월동율은 품종 간에 차이가 있었으며(p<0.01), 외국품종보다 국내품종이 대부분 월동이 잘되었다. 조생종에서는 코윈어리의 월동율이 68%로 가장 높았고, Florida 80의 월동율이 35%로 가장 낮았으며, 중생종에서는 코윈마스터의 월동율이 70%로 가장 높았고, 외국품종인 Tam 90이 42%로 가장 낮았다(p<0.05). 외국품종 중에서는 Winter-hawk가 57% 월동하여 다른 외국 품종보다 월동율이 높았다. 건물수량은 품종 간 차이가 많았다(p<0.05). 조생종 중에서는 내한성이 강한 국내품종인 그린팜 2호와 코윈어리가 외국품종인 Florida 80보다 건물수량이 약 3~3.5톤/ha 많았다. 중생종 중에서는 내한성이 강한 국내품종인 코윈마스터가 외국품종인 Winter-hawk나 Tam 90보다 건물수량이 약 2톤/ha 많았다(p<0.05). 이탈리안 라이그라스의 내한성은 건물생산성과 고도의 정의상관이 있었다(p<0.01). 우리나라 중부지역에서 이탈리안 라이그라스를 5월 중순에 수확을 완료해야 한다면, 내한성이 강한 국내 육성 조생 또는 중생품종을 선택하여 재배하는 것이 양질 조사료 생산성 향상에 유리할 것으로 사료된다.

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold (4°C), salt (NaCl 200 mM) and heat (42°C) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

Glutathione S-transferase (GST) is a key gene involved in multiple stress tolerance in all living organisms, though it is still to be disclosed the gene function in teff grass [Eragrostis tef (Zucc.)Trotter].The objectives of this study were to clone and molecular characterization of GST gene in teff grass. We characterized GST1 from teff grass (EtGST1), it composed of a 645-bp open reading frame (ORF) that encoded 195 amino acid residue. Further, we transformed EtGST1 in E.coli BL21 (DE3) cells. This recombinant EtGST1 in E.coli BL21(DE3) induced at 37°C temperature. In addition, Growth of cells overexpressing EtGST1 rapidly increased in the presence of polyethylene glycol (5%), heat (46°C), NaCl (0.6%), and arsenic (1 mM) than that of cells harboring an empty vector. These results suggest that EtGST1 would be suitable candidate for improving tolerance in forages and/or grasses species against multiple abiotic stresses.