Biological activities of bombolitins from Bombus ardens, B. consobrinus, B. terrestris and B. ussurensis (bombolitinsA, C, T and U, respectively) were examined using hemolytic, anti-microbial, anti-fungal and anti-tumor activity assays.Among the four bombolitins tested, bombolitin T showed the highest hemolytic and anti-tumor activities. All bombolitinsexhibited strong anti-microbial and anti-fungal activities, and bombolitin A specifically possessed the highest anti-microbialactivity against the Gram-negative bacteria Escherichia coli. Circular dichroism spectrometry analysis revealed that allfour bombolitins had over 61.7% and 45.5% of α-helicity in 30 mM sodium dodecyl sulfate and 50% trifluoroethanolbuffers, respectively, which form lipid-membrane-mimicking environments. Bombolitin T showed the lowest IC50 valuesof 8.5 μM and 8.8 μM against SK-OV-3 and NIH-OVCAR-3 cell lines, respectively, after 72 h of treatment, but itsrelative hemolytic activity at a concentration of 200 μM was 2.3-fold higher than that of 0.1% Triton X-100. Thisstudy provides new information on the biological and molecular properties of venom peptides of bumblebees. However,further studies on reducing cytotoxicity of bombolitins are needed for designing selective anti-tumor peptides.

The attraction of the tomato whiteflies Trialeurodes vaporariorum, to four type of traps combined with a colored stickyboard and an light emitting lamp was investigated in 2016 in an tomato greenhouse. The colors of the sticky boardswere yellow and the peak wavelengths of the light emitting traps were 470nm(blue), 590nm(yellow) and 450~625nm(white).The type of traps was designed to directly illuminate the plants with an light emitting lamp and set along the lowerpart of the crops whose each sticky board was directed to the plants. This experiments showed that T. vaporariorumadults on average preferred (p>0.05) traps in blue lights(110±3.2adults/trap) compared to traps in yellow lights (71±1.4adults/trap)and white light traps(45±1.1adults/trap). The blue light trap(470nm) showed the most attractive to T. vaporariorum adults,followed by a similarly attraction to the yellow light trap(590nm), whereas the control (no light trap) was little attractiveto T. vaporariorum adults.

NRPS (non-ribosomal pepetide synthetase) plays an important role in the biosynthesis of secondary metabolites, which includes economically important compounds such as antibiotics, antitumor agents, immune-suppressants, siderophores, toxins, etc. In most cases, however, their functions and biosynthesizing pathways have been still remained riddles because of their cryptic expression and recalcitrant genetics. In our previous genome analyses of Cordyceps bassiana C101, we predicted the sequences of secondary metabolite biosynthesizing gene clusters, which contain non-ribosomal pepetide synthetases whose functions are already known such as beauvericin, bassianolide, bassiatin, tenellin, beauverolides, and oosporein. The authors focused on nrps6 that has not been functionally studied. The single-gene deletion and overexpression mutants were established via Agrobacterium-mediated transformations. PCR, quantitative real-time PCR, and southern blot analyses were carried out to confirm the correct single-gene integrated homologous recombination. In further study, comparative metabolic profiling between wild-type and mutant strains will be followed by their detailed chemical structure analysis through NMR spectroscopy.

Regarding the Forty-eight Poems of Soswaewon by Haseo Kim In-Hu, this study examines the soundscape's sound source type and receiver setting at the time of Soswaewon Garden landscaping and how the function and meaning of garden sounds are produced and expressed through literature and on-site survey. The main results of the analysis are as follows. Regarding the soundscape’s main receiver setting in Soswaewon Garden, area at stream garden, Chojeong, Jewol Pavilion, and Gwangpung Pavilion and a display stand were identified, and a combination of various forms of water sounds and artificial sounds around the mountain stream and musical panorama of nature are the sources of sound. Diversity Soswaewon’s soundscape acts as an important landscape resource for playing, sightseeing, and appreciating Soswaewon Garden. In the Forty-eight Poems of Soswaewon, the methods of enjoying the landscape were the act in which the scholars’ view of life and nature are contained at the time of garden landscaping. Therefore, it is of great significance. The Forty-eight Poems of Soswaewon is a textbook about old scenery in which real landscape and semantic landscape are substituted by connecting various sceneries of the season to scholars’ experience of taste for the arts through borrowed scenery and borrowed sound. The soundscape in the Forty-eight Poems of Soswaewon intactly contains the taste of entertainment in appreciation of the garden’s scenery of the season and scholars’ self-cultivational practice for mind and body called self-projection and reflection by having the flow of the mountain stream as the focal point of the garden. The fact that Soswaewon’s soundscape was of as great importance as visual landscape implies that it has significant implications for modern landscape design.

Pleurotus eryngii, an edible white-rot fungus, is cultivated widespread in Eurasia, northern Africa and China. It occupies the second position in the world mushroom market. Despites the importance, the small numbers of studies have been done. To promote the availability of P. eryngii, it is important to know the genome and organization of the fungus. In this study, the whole genome sequence of P5 monokaryon from P. eryngii KNR2312 strain was sequenced using Next Generation Sequencing (NGS) strategy. Identified 222 SSR markers based on newly known genome information and various type of markers used to construct the map consisted of 12 linkage groups (LGs) spanning 1047.8 cM. Using composite interval mapping, 71 quantitative trait loci (QTL)s were identified on 12 linkage groups (LG) for nine traits such as yield, quality, cap color and earliness in four different populations. Clusters of more than five QTLs for various traits were identified on three genomic regions on LG 1, 7, and 9. The largest cluster was identified on LG 1 in the range from 65.4 to 110.4 cM. We performed the genes prediction analysis responsible for yield in the LG1 genomic region with highest logarithm of the odd (LOD) scores The identified genes were involved in biomass degradation and synthesis and signal transduction. However, the region was wide to identifie the genes crucial role in important trait, we need to narrow down. To improve the resolution of QTL mapping, we enlarge the populations crossing with additional 205 monokaryons. Find mapping of QTLs will increases the accuracy and efficiency of interpret the genomic region and enhance the usefulness of genomic information. [Supported by a grant from the IPET (213007-05-1-SBI30), MIFAFF, Republic of Korea.]

Sanghuangporus sanghuang (Hymenochaetaceae, Agaricomycetes) is a medicinal mushroom which has gained particular attention in Korea as a natural immune booster and a cancer suppressor. Recent molecular phylogenetic studies clarified the species concept of S. sanghuang extensively used in Asia including Korea, China, and Japan. In this study, we assembled its mitochondrial genome and identified the locations and functions of genes and coding regions using next-generation sequencing. Raw sequencing data was produced using PacBio approach, and assembled through hierarchical genome assembly process (HGAP). Determining the mitocondrial regions GeSeq and Artemis annotation tools were used. And the resulting gene map was visualized using OGDraw software. A total of 108 reads was aligned with a coverage of 11.87X. The entire mitochondrial genome was 111,734 bp in length, in which 24 proteins and 46 transfer RNAs were recognized. The overall base composition is 38.37% A, 38.42% T, 11.05% C and 12.14% G with an GC content of 23.2%. This mitochondrial genome data can be used to understand its phylogenetic relationship and taxonomic resolution and as a source of markers for population and species delimitation.

The tobacco cutworm, Spodoptera litura(Fabricius), is a major pest of tomato and frequently demands control measures. The timing of insecticide application is a key factor in determining its efficiency, so an experiment was designed to investigate this. Application of insecticide was based on three criteria: (i) the number of trap-caught moths in a Delta-type trap with a commercial sex pheromone lure placed in the center of the target area, soon after plant emergence; (ii) the percentage of plants exhibiting pinhole-type damage (10% or 20%) and (iii) the percentage of plants exhibiting shot hole-type damage (10% or 20%) compared to a check plot without any control measures. We found that the number of trap-caught moths was, compared to the other methods, the best means of deciding on insecticide application in tomato plant to control the tobacco cutworm. Using pheromone traps, we obtained the best performance of the insecticide Shinnago, causing > 90% larval mortality. Without insecticide application, tomato yield reduction due to the tobacco cutworm larva damage was 27%.

목 적: 안경과 콘택트렌즈 구매장소에 따라 안경사의 직무신뢰도 및 안경과 콘택트렌즈 가격의 만족도에 대한 의견 차이가 있는지에 대하여 알아보고자 하였다. 방 법: 2015년 5월에서 6월 두 달 동안 대구·경북, 부산·경남지역의 안경광학과 재학생과 고객을 대상 으로 안경과 콘택트렌즈 구매 장소, 안경사의 판매 및 검안에 대한 신뢰도 및 안경과 콘택트렌즈 구매가격 만족도에 대한 설문을 실시하였다. 결 과: 직무신뢰도에서 검안은 남자의 평균값이 가장 높았고(3.89±0.88), 가격만족도에서는 모두 여자 의 평균값이 높았다(3.18±0.72). 구매장소에 따른 상관관계에서는 비 프랜차이즈의 검안신뢰도가 가장 높 았다(3.99±0.91). 결 론: 구매장소에 따른 직무신뢰도와 가격만족도에 대하여 일부 항목에서 유의한 차이가 나타났고, 이 결과를 바탕으로 직무신뢰도와 가격만족도를 높이는 자료로 이용될 수 있을 것이라고 생각한다.

Isaria farinosa (Hypocreales, Ascomycota) is a cosmopolitan entomopathogenic fungus affecting a wide range of arthropod hosts. It has mainly been studied as a insecticidal agent to control the agricultural pests. To investigate the useful secondary metabolite(SM) genes in Isaria farinosa C1012 strain, de novo assembly and genome mining were carried out. A whole genome sequencing with PacBio RSII system generated NGS reads greater than 4Gb, which were assembled into 16 contigs using FALCON program. The total size of genome was 33.36Mb. The N50 and N90 were 6,686,213 and 1,912,865bp, respectively. The assembled genome data was analyzed with antiSMASH3 program with a default setting to localize the gene region responsible for synthesizing SMs, such as non-ribosomal peptide synthetases (NRPS) and polyketide synthase (PKS). In this study, we predicted 16 NRPS, 13 PKS, and 9 PKS-NRPS hybrid gene clusters in I. farinosa genome.

Using a partial D-loop sequence of mtDNA, a comprehensive molecular evolutionary analysis was performed of the maternal lineages of the Jeju native pigs(JNPs) that presented in Jeju Island. A total of 100 DNA sequences from Asian domestic pigs(ADP), European domestic pigs(EDP), Asian wild boars(AWB), European wild boars(EWB), and JNPs were used for the molecular evolutionary analyses including phylogeny and network analyses. The most fitted model for the phylogenetic analysis was determined using hierarchical likelihood-ratio tests conducted by MrModeltest implemented in the PAUP package. A consensus tree was established from 5,000,000 iterations using the MR BAYS program. Three recognizable JNP groups–one(JNPE) in the European cluster and two(JNPA1 and JNPA2) in the Asian cluster–were detected in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the most adjacent to that of EWB and a clear haplogroup sharing an identical haplotype(hap16) among 15 individuals of JNPE and 2 individuals of EWB was detected.