국내 유통되는 반려동물 사료의 살모넬라 분석시 증균 배양법, 효소면역기법에 의한 분석, 종 특이 primer를 활 용한 PCR 방법을 활용하여 비교 평가하였다. 시료 175점 Salmonella spp. 검출 결과 증균배양법 및 종 특이 primer를 활용한 PCR 방법에 의한 검출 방법에서 2점의 시료(육포, 옥수수 글루텐)가 양성으로 확인되었고, 효소면역기법에 의한 검출방법에서는 1점의 시료(옥수수 글루텐)가 양성 으로 확인되었다. 증균배양법 및 효소면역기법에 의한 검 출방법에 비해 종 특이 primer를 활용한 PCR 방법을 적 용 할 경우 시료에서 분리된 균주의 종(species) 판별이 가 능하였다.
이 연구는 전통미술놀이를 통해 초등학생들의 공감능력 향상을 목적으로 한다. 서울시 소재 초등학교 5학년 학생 52명을 통제집단과 실험집단으로 나누어 사전-사후 인성공감능력(도덕적 공감, 감성적 공감, 사회적 공감)과 미적공감능력(사고개방성, 독립성, 심미성) 설문을 통해 조사하였고, 그 결과를 통계분석 프로그램 SPSS 22.0 프로그램을 이용하여 분석하였다. 또 포트폴리오 및 수업활동자료, 소감문, 관찰을 통하여 이 연구의 효과에 대한 질적 검증을 실시하여 최종 결론을 도출하였다. 연구 결과, 전통미술놀이 프로그램에 참여한 학생들은 통제집단 학생들에 비해 도덕적 공감, 감성적 공감, 사회적 공감, 사고개방성, 독립성, 심미성이 모두 유의미하게 향상되었다. 따라서 본 프로그램은 학생들의 공감능력 키우기에 긍정적인 영향을 미치는 것으로 확인되었다. 이 연구는 전통미술놀이 활동이 우리 미술문화에 대한 이해를 바탕으로 4차 산업혁명시대의 미래교육 방향으로 제시한 인간의 정서적 소통과 공감능력 함양에 긍정적인 영향을 미친다는데 의의가 있다.
Barnyard grasses are dominant weed that are not eliminated easily in the direct water seeding cultivation. So, deep water treatment can reduce their growth at the early growing stage of submerged paddy. This study was carried out to investigate the reducing growth of the barnyard grasses through flooding at seedling stages of rice plant in the green house. Under the normal condition, the plant height of rice variety, Samgwangbyeo, and 3 species of barnyard grass, E. caudata, E. pratocola and E. utilis were not showed the difference up to 10 days after seeding while the plant height of 3 barnyard grass species, especially E. utilis, was more elongated than the rice at 25 days after seeding. Plant height of the 3 barnyard grasses were not elongated largely during 3, 5 and 7 days of flooding treatment at 10 days after seeding. Interestingly, the rice seedlings was grown over 20 cm, and the flooding tolerance of rice seedling was higher than the 3 barnyard grass varieties. However, after flooding treatment for 3, 5 and 7 days, the elongation of plant height of 3 barnyard grasses, especially E. utilis was more speedy compared to rice seedlings as 6 to 9 days passes. And the protein spots from barnyard grasses were also reduced and eliminated more than the spots of rice seedling after flooding treatment.
Light is a energy source for photosynthesis og green plant and sources for photoperiodsm and photomorphogenesis. Plants have showed a variety of reactions corresponding to the surrounding environment to survive, and have introduced environmental signal into growth development. Rice yield has reduced by increasing localized heavy rain that leads duration of solar intensity to decrease 10-16% during cultivation period. It is necessary to improve adaptive technology and observe change of rice yield and growth by decrease of solar intensity for safe culture of rice. For these reasos, we carried out shade culture experiment and compared to rice growth and yield for two rice varity(Dasanbyeo, Chuchengbyeo) in 2008, and we also performed shade culture experiment at two growth stage(panicle formation stage, booting stage) in order to research rice growth and yield by decease of solar intensity on 2009. We carried out shade treatment to make different solar intensity under three different conditions; control, 30-40% block, 70-80% block using shade net. As a result, experiment result showed that plant height and SPAD value were more inceased and tiller number was more deceased under shade treatment than control. Shade treatment decreased rice yield by reducing panicles number per m2 spikelet number per panicle, and percent ripened grain of yield component. The results of protein analysis showed difference between control and treatment.