A 9-year-old, intact female, Pungsan dog was referred for purulent vaginal discharge and depression. Abdominal radiographs revealed gas-filled and soft tissue opacity tubular structures in the mid to caudal abdomen. On ultrasonography, fluid-filled uterus and cervix accompanied by hyperechoic foci and reverberation artifacts were identified. Multiple hyperechoic foci were found within the uterine wall, indicating gas content. Escherichia coli was isolated from aerobic and anaerobic bacterial cultures. Radiological differential diagnosis of tubular shaped, dilated gas-filled structure, and gas in the wall of the structure should consider emphysematous pyometra with endometrial pneumatosis in intact female dogs with vulvar discharge.

Pseudo-pheochromocytoma is defined as an adrenal tumor that has the typical characteristics of pheochromocytoma likely hypertension but is histopathologically diagnosed to other types of adrenal tumors. 11-year-old, castrated male Maltese presented for a left adrenal mass with hypertension. According to clinical signs and radiological features, a temporary diagnosis was made of pheochromocytoma. The adrenalectomy was performed and the histopathological examination of the resected adrenal gland was diagnosed as cortical adenocarcinoma. This report describes the case of pseudo-pheochromocytoma in a dog and implies the salience that even if pheochromocytoma is diagnosed based on all test results, pseudo-pheochromocytoma should not be excluded.

A 13-year-old mixed dog was referred to the animal medical center, Gyeongsang National University. Lung masses were diagnosed at the left cranial and caudal lobes through diagnostic imaging, and consequently, left pneumonectomy was performed using a self-cutting linear endoscopic stapler. The pulmonary arteries, veins, and bronchus of each lung lobe were sealed and resected at once, and any air leakage or bleeding was not observed after the surgery. Compared to the conventional ligation method, the self-cutting linear endoscopic stapler has the advantage of significantly reducing the operation time and enabling simple and reliable sealing.

Even though klotho deficiency in mice exhibits multiple aging-like phenotypes, studies using large animal models such as pigs, which have many similarities to humans, have been limited due to the absence of cell lines or animal models. The objective of this study was to generate homozygous klotho knockout porcine cell lines and cloned embryos. A CRISPR sgRNA specific for the klotho gene was designed and sgRNA (targeting exon 3 of klotho) and Cas9 RNPs were transfected into porcine fibroblasts. The transfected fibroblasts were then used for single cell colony formation and 9 single cell–derived colonies were established. In a T7 endonuclease I mutation assay, 5 colonies (#3, #4, #5, #7 and #9) were confirmed as mutated. These 5 colonies were subsequently analyzed by deep sequencing for determination of homozygous mutated colonies and 4 (#3, #4, #5 and #9) from 5 colonies contained homozygous modifications. Somatic cell nuclear transfer was performed to generate homozygous klotho knockout cloned embryos by using one homozygous mutation colony (#9); the cleavage and blastocyst formation rates were 72.0% and 8.3%, respectively. Two cloned embryos derived from a homozygous klotho knockout cell line (#9) were subjected to deep sequencing and they showed the same mutation pattern as the donor cell line. In conclusion, we produced homozygous klotho knockout porcine embryos cloned from genome-edited porcine fibroblasts.