T:raumat ic eosinophili c granuloma(TEG) of the oral mucosa is considered to be a reactive benign lesion. which commonly manifests as an ulcer with elevated and indurated borders Clinically, this lesion simulates a malignant tumor. Histology shows a diffuse. dense, polyrnorphic, and eosinophil - rich cellular infiltra te. which extends deeply into the underlying soft t issues. A major constituent of infil trates is a population of mitotically active‘ la rge‘ atypi cal mononuclear cells. Immunohistologic evaluation of the large atypical cells has suggested a myofibroblastic 01' his tiocytic ol'igin. However, recent reports have shown that these cells are positive 1'01' CD30 antigen and it has been s llggested tha t a subset of TEG cOllld be included within the spectrum of CD30+ lymphoprolifer ative disorders. We have described 2 patients who had oral mllcosal lesions with features of TEG. ln patient 1. the lesiona l cells expressed CD3 .. CD43, LCA. Interestingly, the large cells were strongly CD30 positive. bl1t nega tive for CD68, CD45Ro‘ CD56, CD20. This case was interpreted as a CD30+ Iymphoproliferative disorder. In pa tient 2‘ t he la rge cell s showed strong posit ive for CD68, hut negative for CD30 The small lymphocytic cells ex prerssed CD3 This case was interpreted as an atypical histiocytic granuloma. Therefore, TEGs inclllde atypical histiocytic granllloma a long with the CD30+ lymphoid lesions. These findings suggested that TEG w0111d be a hete rogenous category of oral mucosal di sorders

Gudul has been used since it was invented, and will be used continuously. But recently, we used rarely. One of the this reason is unequally floor temperature in Gudle because the distribution of heating air is not constant. So flow field in Gudul must be known for uniform temperature in floor. Recently, many fluid engineer have been performed the numerical analysis for the understanding of thermal and flow field based on the development of CFD(Computational Fluid Dynamics). In this paper, to find out the variation of temporal temperature in floor of traditional Gudle, we have performed numerical analysis. Initial temperature in floor is 20 t:' after 880 seconds later, maximum temperature become 2i.Oi t:'. ihour later, maximum temperature in floor become 34.5 t:' because continuously increase from 880 seconds.

Hereditary dentin defects consists of dentin dysplasia(DD) and denti nogenesis imperfecta(Dr) ‘ The Dl associated with osteogenesis imperfecta has been classified as DI type 1. whereas isolated inherited defects have been categori zed as DI types II and III , However‘ whether DI type III should be considered a distinct phenotype 01' a variation of DI type 1I is debatable , Recent genetic findings have focused attention on the role of the dentin sialo phosphoprotein(DSPP) gene in the etiology of inherited defects of tooth dentin, We have identified novel mlltation( c,727G - > A, p,D243N) at the 243th codon of exon 4 of the DSPP gene in a Korean patient with DI type III The radiographic and histologic features of the patient revealed the classic phenotype of shell teeth These findings sllggest that DI type II and III are not separate diseases bllt rather the phenotypic variation 01' a s ingle disease

This study aimed to investigate the effect of different light-emitting diode (LED) on the stress response of workers before and after a flower arrangement (FA) activity. Participants were 16 office workers who performed FA under 7 out of 8 LED lighting conditions: darkness, white (at correlated color temperature of 4,500 K), red, blue, green, magenta, cyan, and yellow; no FA is performed in the darkness condition. To identify significant differences among the different lighting conditions, the heart rate (HR), blood pressure (BP), heart rate variability (HRV) consisting of standard deviation of the normal-normal intervals (SDNN), total power (TP), ratio of the low and high frequency (LF/HF), and blood pressure were measured before and after FA. The salivary cortisol level was analyzed immediately after FA. The correlations among HR, HRV, and cortisol showed that the blue and green lights positively affected stress-responses and caused considerable reduction of stress. However, there was only a minor significant difference among other lighting colors, although individual lights may impact one or more parameters. These findings reflect that the methodologies and results of this study can be effectively used to achieve a highly active horticultural therapy with the constructive assistance of LED lighting colors.

Backgoound : This study was conducted to evaluate the quality variation of Ixeris dentata on the antioxidant contents and antioxidant activities according to the different producing area. Methods and Results : The samples were extracted with 70 % EtOH and then analyzed for total flavonoid contents, polypenol contents, DPPH radical scavenging activity, and ABTS radical scavenging activity. Luteinol 7-O-β-D-glucoside, an index component of the Ixeris dentata, was analyzed by HPLC. The leafs of Ixeris dentata in Jinan had the highest concentration of polyphenols (23.91 ㎎/g), followed by Jinbu (22.63 ㎎/g) and Eumseong (21.36 ㎎/g). Flavonoid content was highest in Jinan (15.27 ㎎/g), but there was no significant difference between Jinbu (14.05 ㎎/g) and Eumseong (13.99 ㎎/g). The contents of luteinol 7-O-β-D-glucoside were confirmed in Jinan (0.68%), Jinbu (0.49%) and Eumseong (0.36%), respectively. Conclusion : The comparison of antioxidant contents and antioxidant activities of Ixeris dentata according to the different producing area, Jinan was had the highest concentration, followed by Jinbu and Eumseong. Our results showed that the content of luteoline-7-D-glucoside varied among the different producing area.

Perilla frutescens (L.) is an annual herbaceous and ornamental plant in the Lamiaceae family. Perilla frutescens (L.) Britt.cv.Chookyoupjaso were irradiated using a 200 Gy gamma ray in 1995. By HPLC analysis, this new cultivar significantly induced isoegomaketone content compared with ‘Chookyoupjaso’ control. The phenotypical difference was the changed leaf color of the ‘Atom-Ketone’ from violet to green. The yield potential of this cultivar (106 kg/10a) was 1.83 folds higher than that of ‘Chookyoupjaso’ (57.65 kg/10a). The methanol extracts of ‘Atom-Ketone’ inhibit nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. This extract was further partitioned using ethyl acetate (EtOAc), butanol (BuOH), and water. The EtOAc fraction (EF-Atom-Ketone) was evaluated for antiinflammatory activities. These results indicated that the EF-Atom-Ketone reduced NO production by inhibiting inducible nitric oxide synthase (iNOS) expression. The EF-Atom-Ketone treatment also significantly diminished expression of MCP-1 and IL-6. Therefore, ‘Atom-Ketone’ reveals the potential therapeutic use of bioactive

Perilla frutescens (L.) is an edible plant, not only used as s food ingredient, but also in skin cream, soaps, and medicinal preparetions. ‘Atom-Ros’, a perilla (Perilla frutescenc (L.) Britt. cv. Chookyoupjaso was developed in 1995 by 200 Gy gamma irradiation-mutagenesis. This new cultivar has high rosmarinic acid content more than two fold compare with ‘Chookyoupjaso’ control. The observed phenotypical difference was changed leaf color of the ‘Atom-Ketone’ from violet to green. The yield potential of this cultivar (123.5 kg/10a) was 2.14 fold higher than that of ‘Chookyoupjaso’ (57.65 kg/10a). The methanol extracts of ‘Atom-Ros’ were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophase cells. Atom-Ros showed significant inhibition of NO production. This rosmarinic acid extracted from ‘Atom-Ros’ has a good potential to be developed as an antioxidant agent.

VitE (tocotrienols and tocopherols) are micronutrients with antioxidant properties synthesized by photosynthetic bacteria and plants that play important roles in animal and human nutrition. A new mutant line, T1001-1, was isolated from in vitro mutagenized population by ionizing radiation and shown to have increased VitE contents. The total VitE content was 26% increased in the T1001-1 mutant seeds compare with cv. Dongan (wild-type). In addition, we showed that the mutant confers retarded seedling growth during the early seedling growth stage in rice. To study the molecular mechanism of VitE biosynthesis, we used the rice microarray to identify genes that are upor down-regulated in T1001-1 mutant. In addition, we identified differentially regulated pathway using MapMan analysis, which provides deep insight into changes in transcript and metabolites. Our results enhanced the transcription of genes involved in starch and lipid metabolism in T1001-1 mutant. To identify the molecular mechanisms of the events involving transcription factors in tocopherol accumulation, we compared the expression patterns of transcription factors. The AP2-EREBP, WRKY, C2H2 transcription factor were up-regulated, whereas the MYB family was down-regulated in T1001-1 mutant. Our results demonstrate change of important transcript in high level of VitE accumulating rice mutant.

Ionizing radiation is known to cause chromosomal alterations such as inversions and deletions and affects gene expression within the plant genome. To monitor the genome-wide transcriptome changes by ionizing radiation, we used rice Affimetrix GeneChip microarray to identify genes that are up- or down regulated by gamma-ray (200 Gy, 60Co source), cosmic-ray and ion beam (40 Gy, 220 MeV carbon ion). The overall expression patterns between gamma-ray and ion beam were similar but cosmic-ray was regulated differently. Combined results from all 3 radiations identified 27 up-regulated genes and 188 down regulated genes. These results mean the induction of similar mechanism changes in treatments of gamma ray and ion beam. However the different expression in treatment of cosmic-ray might be due to the other environmental conditions. Among the commonly up- or down- regulated genes, we chose highly up- or down- regulated several genes and confirmed its regulation in response to ionizing radiation exposure by RT-PCR analysis. Moreover, we showed that specific co-expression networks of candidate radio marker genes by ARACNE algorithm. Our results present profiles of gene expression related to different ionizing radiation and marker gene to predict sensitivity to ionizing radiation, such as GS (glutelin subunit) and FBX322.

Cryopreservation has been known as an efficient method for long-term preservation of clonally propagated plants, and several cryopreservation methods have been developed. Among them, a droplet-vitrification method for potato using axillary shoot tips in vitro has been established previously. In this study, we have optimized the procedure in which explants were submitted to a step-wise pre-culture in liquid sucrose-enriched medium (0.3 and 0.7 M for 7 and 17 h, respectively). The pre-cultured explants were dehydrated with PVS3 (w/v, 50% glycerol + 50% sucrose) for 90 min or modified PVS2 vitrification solution (w/v, 37.5% glycerol + 15% DMSO + 15.0% ethylene glycol + 22.5% sucrose) for 30 min. This two dehydration solutions produced post-cryopreservation regeneration percentages of 57.2% and 80.9%, respectively. We also compared a new post-culture medium (0.1 mg L ･ -1 GA3, 0.1 mg L ･ -1 kinetin) with the conventional one (0.15 mg L ･ -1 IAA, 0.2 mg L ･ -1 zeatin, 0.05 mg L ･ -1 GA3); the shooting initiation rates were 80.9% and 43.5%, respectively. The results suggest that the modified droplet-vitrification protocol described in this study is more effective, easier to implement, and more economical than the droplet-vitrification protocols currently used for potato.