Basic dyes such as malachite green and methylene blue have been used as disinfectants to control water fungal infections since the 1930s. However, after succeeding reports of carcinogenicity and bioaccumulation of the dye, their use was forbidden in lieu of public health. This study undertook to evaluate the therapeutic effect of sulfur solution processed by effective microorganisms (EM-PSS) against Saprolegnia parasitica infection, and its safety in fish. In vitro antifungal evaluation of EM-PSS inhibited the growth of S. parasitica mycelia at concentrations of 50 ppm or higher. The acute toxicity test of EM-PSS to the mud fish (Misgurnus mizolepis) measured a no effect concentration (NOEC) at 100 ppm, the lowest effect concentration (LOEC) at 125 ppm, and the half-lethal concentration (LC50) at 125 ppm in juvenile and 250 ppm in the immature stage. In addition, the ecotoxicity test of EM-PSS using Daphnia magna inhibited swimming of D. magna at concentrations of 100 ppm or less. Lastly, the EM-PSS prevented infection of S. parasitica to mud fish, at concentrations of 50 ppm. Furthermore, at 100 ppm concentration, the EM-PSS showed no acute toxicity on mud fish, nor any eco-toxic effects on D. magnano. Therefore, we conclude that carcinogenic disinfectants such as malachite green and methylene blue could be replaced by EM-PSS to remove S. parasitica in mud fish farming, and might be a potential eco-friendly disinfectant in aquaculture.

In this study, changes in daily temperature range were investigated using daily maximum and minimum temperatures of Busan and Daegu for last 81 years (1934-2014), and also characteristics of daily temperature range and seasonal fluctuations by urbanization were examined. First, elapsing changes showed a lower decreasing trend in Busan (0.32℃) than Daegu (1.2 8℃) for last 81 years. Daily temperature range showed the highest rise in winter in both Busan and Daegu. Second, daily temperature range due to urbanization showed that Busan had a pronounced decreasing trend before urbanization meanwhile Daegu showed the same trend after urbanization. On seasonal changes, the results of Busan showed a decreasing trend in summer before urbanization, and in autumn after urbanization. For Daegu, the results showed a decreasing trend in spring before urbanization, and in winter after urbanization. Seasonal fluctuations of Busan showed little difference in the pre and post-urbanization, except in winter and summer. There was large difference in daily temperature range in winter after urbanization, and in summer before the urbanization. The results in Daegu showed that there was decreasing trend of daily temperature range in all seasons after urbanization.

It is well known that urban relative humidity has continuous decreasing trend owing to the influence of urbanization. The change of relative humidity is directly influenced by two factors, namely, temperature effect and water vapor effect in various urban effects. In this study, the temperature and the water vapor effects on the relative humidity change were analyzed by using monthly mean relative humidities for a long period(1961～2013) in Busan and Daegu. The major results obtained in this study can be summarized as follows. Firstly, the urban dryness was caused mainly by water vapor effect in summer. But, for the other seasons, the urban dryness is mainly due to the temperature effect. Secondly, the relative humidity in Busan is on the decrease until now. This phenomenon is similar to another Korean huge cities such as Seoul, Daejeon and Incheon.