Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA (chitosan+hyaluronic acid) and GnHG (chitosan hydrogel) as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL), respectively. Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. CASA analysis showed GnHA and GnHG are effective against cryopreserved boar sperm. And antioxidant effect is measured by lipid peroxidation analysis. GnHA and GnHG, which is chitosan complex are effective for boar sperm cryopreservation by antioxidant effect.

Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA and GnHG as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function. Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL). Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa. Sperm motility was higher in GnHA 0.25 mg/mL and GnHG 0.5 mg/mL compared to control. In addition, GnHG 0.5 mg/mL was significantly decreased in lipid peroxidation analysis. The results suggest that GnHA and GnHG are effective for boar sperm cryopreservation by antioxidant effect.

The resistance of maize weevil(Sitophilus zeamais), an important agricultural pest of stored grain, on aluminium phosphide fumigant(PH3) has been reported recently in Korea. Many researchers reported that ethyl formate(EF), a potential methyl bromide alternative fumigant, showed good efficacy on several grain pests. In this trial, based on CTP (concentration × time products), we evaluated efficacy of EF on phosphine resistant and susceptible maize weevil. Each of insect strains were collected in grain storehouses and examined the PH3 resistance according to FAO method (Busvine, 1980). L(Ct)90 of PH3 resistant and susceptible strain was 48.3 and 0.3 g h m-3, respectively. When treated with EF, L(Ct)90 of PH3 resistant and susceptible strain was 90.0 and 110.9 g h m-3, respectively. In the case of EF, there was no difference between resistant and sucsecptible strain compare with PH3. These results indicates that EF is a potential alternative for aluminium phosphide resistance management in grain storehouses.

Exportation of sweet persimmon has been grown up recently, but the growth was accumulated because of quarantine and shelf life issues. Herein, we've investigated the concurrent processing of ethyl formate (EtF), a MeBr alternative quarantine fumigant, and 1-methylcyclopropene (1-MCP), an anti-ethylene compound, on sweet persimmon and suggested proper fumigation methods. An important quarantine pest, Tetranychus urticae, was inoculated on sweet persimmon, and then 70 g m-3 of ethyl formate has been fumigated for 6 h before and after 1 ppm of 1-MCP treatment for 24 h at 5°C condition. These concurrent processing showed the promise that efficacy of EtF was enough to meet quarantine guideline and shelf life of fruits was extended in terms of the hardness, surface color and sugar contents when treated fruits were stored for four weeks at 5°C.

Methyl bromide (MB) fumigation is still allowed to use in quarantine treatment on imported timbers and logs, even though IPPC(International Plant Protection Convention) was strongly recommended to reduce their use in case of existing alternatives. A newly developing MB alternative fumigant, ethanedinitrile (EDN), showed the potential in terms of better efficacy to various timber pests and penetration into the timber than MB. Herein, for suggestion of quarantine guideline in Korea, we had verified the efficacies under different temperature conditions(5, 5-15, >15℃) and monitored TLV(Threshold limited value) of EDN post-fumigation for worker safety. The 6 scheduled doses of EDN depending on types of timber pest and temperature conditions had evaluated with PVC-Tarp tent(>100m3) with 40-50% loading factors of imported logs. To control normal wood related pests such as Japanese termite (Reticulitermes speratus) and bark beetle(Cryphalus fulvus), the scheduled doses 30, 40 and 40 g/m3 for 24hr fumigations showed >99% efficacy at 5, 5-15 and >15℃, respectively. The scheduled doses 100, 120 and 150 g/m3 for 24hr fumigations was also successful to gain the proved efficacies at 5, 5-15, >15℃, respectively, for the quarantine guideline to notorious wood related pests such as Japanese pine sawyer (Monochamus alternatus) and pine wilt nematode (Bursaphelenchus xylophilus). The recommended ventilation times with atmospheric conditions at ports was > 1 and > 2 hr under the fully uncovered and partially uncovered of fumigation tent conditions, respectively.

본 연구에서는 다양한 처리 온도와 압력 조건에 따라 초임계 이산화탄소(SC-CO2) 처리한 동결 건조 소간의 이화학적 특성을 비교분석하였다. 무기성분은 SC-CO2 처리에 의해 다소 감소한 Cu를 제외하고는 대체적으로 증가하는 경향을 보였다. Vit A는 SC-CO2처리에 의해 다소 감소하였지만, vit B3는 처리에 의해 모든 조건에서 증가하였으며, 특히 45℃, 450bar에서는 13.20mg/100g으로 가장 높게 나타났으며, 처리 전 7.16mg/100g에 비해 크게 증가하였다. 소간 내 지방의 추출 수율은 SC-CO2 처리 시 온도와 압력이 증가함에 따라 높아지는 경향을 보였다. SC-CO2 처리에 의해 취득한 소간유의 지방산 조성을 분석한 결과, 주요 지방산은 Palmitic acid(43.20~55.82%), Linoleic acid(9.25~16.80%) 그리고 Myristic acid(5.16~10.54%)로 나타났다. 또한, 17종의 아미노산을 분석한 결과, 45℃에서 SC-CO2를 처리하였을 때 아미노산의 손실이 적게 일어났었다. 이상의 결과를 종합하여 보면 SC-CO2는 낮은 수분함량에서도 이화학적 특성의 변화를 최소화 하였다. 이에 따라 SC-CO2 처리는 식품산업에서 부산물인 소간을 이용하여 미생물의 오염으로부터 안전한 고부가가치 식품으로써 다양한 가능성을 열 수 있을 것으로 판단된다.

The German cockroach, Blatella germanica, is one of the most important household pest that can cause disease on human. Conventional contact insecticides show less efficacy because it is hard to be contacted or digested by cockroach that lives inside small crevice. Ethyl formate(EF), which is a potential fumigant to control various insect pests such as stored grain insects and quarantine pests, can be penetrated wherever air exist and shows toxicity on respiratory system of insect. In this trials, we've demonstrated practical and commercial scale fumigations of EF on German cockroach. Efficacy of EF was described in terms of concentration × time (CT) product. At practical fumigation, EF gave 100% control of German cockroach nymph and adult when the CT product was 43.2g h m-3. When 600m3 scale warehouse fumigation for 15 hours with 5g m-3 of EF, nymph and adult stage of German cockroach were completely controlled. The ventilation time to reduce EF concentration under TLV(Threshold Limit Value, EF=100ppm) was less than 1 hour. These results show that EF can be applied at warehouse or mill house to control German cockroach effectively.

본 연구는 들기름을 농도별로 배추김치에 첨가하여 6주의 발효기간 동안 pH, 산도, 젖산균수, 산가, 과산화물가, 색도 등의 품질특성과 관능특성을 조사하였다. 또한, 들깨로부터 볶지 않고 냉압착한 들기름과 볶은 후 가열 압착한 들기름을 같은 농도별로 배추김치에 첨가하여 분석하였다. 모든 실험군에서 발효가 진행됨에 따라 pH는 감소하였으며 6주 후에는 최적 pH인 4.48~5.38 범위였고, 산도도 점차 증가하여 1.20~2.09% 범위였다. 젖산균 수는 발효 0~1주 사이에 급격하게 증가하여 발효 6주에는 4.48~6.04 log CFU/g에 도달했으며, 냉압착 및 가열압착 들기름을 첨가하였을 때 젖산균이 더 증가하였다. 산가와 과산화물가는 발효기간에 따라 점차 증가하였으며, 들기름 첨가비율이 높아짐에 따라 증가하는 경향을 보였다. 가열압착 들기름 첨가군에 비해 모든 실험군에서 발효 기간 경과와 더불어 색도 중 L 값과 a 값은 점차 증가하였으며 b 값은 1주까지는 증가하다 점차 감소하는 경향이었다. 들기름을 첨가하여 제조한 김치의 관능평가를 한 결과 5% 냉압착 들기름을 첨가하였을 때 관능성이 가장 우수하였다. 이상의 결과들을 종합하면 배추김치 제조 시 냉압착한 들기름의 첨가는 발효 시 유용한 젖산균을 증가시키고 관능특성을 향상하는 것으로 판단된다.

In this study, activated carbons nanofibers (ACNFs) were prepared from polyacrylonitrile-based nanofibers by physical (H2O and CO2) and chemical (KOH) activation. The surface and structural characteristics of the porous carbon were observed by scanning electron microscopy and X-ray diffraction, respectively. Pore characteristics were investigated by N2/77K adsorption isotherms. The specific surface area of the physically ACNFs was increased up to 2400m2/g and the ACNFs were found to be mainly composed of micropore structures. Chemical activation using KOH produced ACNFs with high specific surface area (up to 2500m2/g), and the micropores were mainly found in the ACNFs. The physically and chemically ACNFs showed both mainly type I from the International Union of Pure and Applied Chemistry classification.

예냉온도와 저장온도가 수출 딸기 ‘매향’의 저장성에 미치는 영향을 알아보고자 본 연구를 수행하였다. 2011년 1월 11일에 숙도 80%의 과실을 경상남도 진주지역의 상업적 온실에서 수확하여 0, 2, 4℃로 2-5시간 동안 예냉한 딸기를 30분 만에 실험실로 수송하여 4, 8, 10℃로 설정된 챔버에 저장하여 실험을 진행하였다. 저장하는 동안 딸기의 무게, 경도, 색도, 당도 및 산도의 변화와 잿빛곰팡이 발생률을 조사하였다. 딸기의 무게는 저장기간 중 모든 온도처리에서 감소하였고, 특히 예냉 및 저장온도가 가장 낮은 처리구에서 품질이 가장 우수하였다. 경도는 예냉온도 4℃, 저장온도 10℃ 처리구에서는 저장 후 10일째부터 급격히 감소하였다. 당도는 가장 낮은 온도인 4℃에 저장하였을 때 우수하였다. 산도는 모든 온도처리에서 증가하였으나 실험종료 마지막 날 4℃ 예냉온도와 10℃ 저장온도 처리구에서 0.7%로 가장 높았다. 잿빛곰팡이는 10℃ 저장에서 가장 많이 발생했다. 따라서 딸기의 신선도를 장기간 유지하기 위해서는 0℃에 예냉하여 4℃에 저장하는 것이 효과적인 것으로 판단된다.

엑스선유방촬영술은 다양한 유방질환 중 석회화 병변을 진단하는데 가장 효과적인 방법이다. 환자의 의 료피폭 저감과 진단에 필요한 최적의 영상을 얻기 위해서는 성능유지를 지속적으로 관리해야 한다. 엑스선 유방촬영술에서 엑스선을 발생시키는 양극의 경사각도는 중심선을 기준으로 하기 때문에 조사야 내의 위 치에 따라 엑스선관의 유효초점이 미세하게 달라질 수 있어 공간분해능의 차이가 발생할 수 있다. 본 연구 에서는 디지털 엑스선유방촬영장치에서 검출기 위치에 따른 LSF를 측정하여 MTF를 계산함으로써 공간분 해능 변화에 대해서 연구하였다. 와이어 직경에 대한 변화에서 50 ㎛ 직경의 와이어의 경우 가장 높은 공 간주파수 값을 나타냈으며 검출기의 픽셀보다 작은 직경을 갖는 와이어를 사용해야 검출기의 공간주파수 에 따른 응답을 바르게 구할 수 있다는 것을 알 수 있었다. 조사야 내의 검출기의 위치에 따라서 공간주파 수는 중심부분의 와이어가 나머지 와이어보다 우수한 MTF 특성을 보였으나, 그 차이는 미소하게 나타났 다. 또한 중앙부분에서 반치폭 또는 유효초첨이 가장 작게 나타났으며 중앙에서 벗어날수록 반치폭이 증가 하여 공간분해능이 낮아진 것으로 나타났다.

Phytochemical investigation of the sesame dregs of Sesamum indicum was conducted by open column and prep-HPLC chromatography. Two phytosterols (1 and 2) and two lignans (3 and 4) were isolated from the MeOH extracts of sesame dregs, and identified as β-sitosterol (1), daucosterol (2), sesamin (3), and sesamolin (4) by spectral analysis. Although these compounds were already isolated from sesame, it is important that they were still main phytochemical components in the sesame dregs.

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of H2O and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and 8.49 μg/mg, respectively), their fermented wine (0.45 and 1.33 μg/mg, respectively) and wine residues (no detection).