We fabricated glucose oxidase (GOx)-modified biosensor for detection of glucose by physical immobilization of GOx after electrochemical polymerization of the conductive mixture monomers of the 3-thiophenecarboxylic acid (TCA) and thiophene (Th) onto ITO electrode in this study. We confirmed the successfully fabrication of GOx-modified biosensor via FT-IR spectroscopy, SEM, contact angle, and cyclic voltammetry. The fabricated biosensor has the detection limit of 0.1 μM, the linearity of 0.001-27 mM, and sensitivity of 38.75 mAM-1cm-2, respectively. The fabricated biosensor exhibits high interference effects to dopamine, ascorbic acid, and L-cysteine, respectively. From these results, the fabricated GOx-modified biosensor with long linearity and high sensitivity could be used as glucose sensor in human blood sample.

아메리카왕거저리(Zophobas atratus)는 대형 거저리류로, 영양적 가치가 높아서 현재는 식용, 사료용 곤충으로 활용하고자 연구가 진행 중이다. 아메리카왕거저리의 산란율 향상을 위해서 산란에 적합한 광주기 조건을 조사하였다. 9L/15D, 12L/12D, 15L/9D (암수비율 1:2, 온도 30 ℃, 습도 65% RH, 조도 1800 lux) 의 광조건 하에서 산란수 조사 결과, 아메리카왕거저리의 산란수은 약 5주간은 증가하다가 이후 서서히 감소하는 경향을 보였다. 아메리카왕거저리 1마리의 일일 평균 산란수은 15L/9D가 22.9±6.5개로 가장 높았고, 그 다음이 9L/15D 16.8±8.5개, 12L/12D 7.5±4.6개 순이었다. 누적 산란수(15주) 역시 15L/9D가 291.1±120.2개로 가장 높았고, 그 다음이 9L/15D 200.9±111.3개, 12L/12D 87.7±64.1개 순이었다. 그러나 폐사율은 9L/15D가 53.3%로 가장 높았고, 그 다음이 12L/12D 50.0%, 15L/9D 43.3% 순이었다. 이런 결과로 볼 때 아메리카왕거저리의 산란에 적합한 광주기는 15L/9D로 판단된다.

We have investigated the most suitable photoperiods for development of Zophobas atratus, super mealworm. The test was conducted with three different photoperiods of 9L/15D, 12L/12D and 15L/9D. The rearing conditions were 30℃, 65% R.H and 1,330 to 1,800 lux with wheat bran. In developmental period of 1 to 15 instars of three photoperiods, 12L/12D showed the shortest developmental period as 79.2±2.6 days, and 9L/15D (82.6±3.1 days), 15L/9D (84.2±2.3 days) in the following order. larval developmental period was statistical significant difference. The death rate was not statistically different. In the body weight, 12L/12D showed the heaviest body weight and 15L/9D, 9L/15D in the following order. But there was no significant difference at body weight. The head capsule, body capsule and body length also showed a similar tendency with body weight. The 79.6% of prepupa time of super mealworm was 15-17 instars. The longer prepupa time was accompanied by the lower daylength condition. In average prepupal period of 15-20 instars, 9L/15D was 17.7±2.5 days, 18.4±2.5 days in 12L/12D, 16.2±3.7 days in 15L/9D. The average of pupal period of female and male was 9.0±1.1 days and 9.6±1.3 days, respectively. In conclusion, the most suitable rearing photoperiod of super mealwarm was turned out as 12L/12D

Part of a field survey to assess the extent of damage by termite (Reticulitermes speratus kyushuensis Morimoto), termite samples were collected from various locations of Korea from 2009 to 2011. During the study period, we firstly discovered Reticulitermes species on Jeju island, and partial sequences of mitochondrial COII gene, which is proved useful to study phylogenetic relationships of termites were investigated. Also, morphological characteristics of the collected samples were compared with previous reports. According to the result of taxonomic study and comparative sequence analysis, it was identified as Reticulitermes speratus kyushuensis Morimoto, and this is first report of R. s. kyushuensis from Jeju island in Korea which can be used as estimation of population structure and tool of control strategy.

In southern region of Korea, double cropping system including soybean as a second crop is widely adopted because prolonged high temperature in October enables soybean maturation even in late autumn. Global warming during decades derives transition of soybean planting dates as late as late-July. Delayed planting beyond the critical time moves reproductive stage to less favorable weather conditions like low temperature and short photoperiod, which can affect soybean yield and seed composition. Therefore selection of appropriate cultivar for changed climate and cultural practice is required. Effects of different eco-type and planting date on soybean yield was investigated for three sowing dates of 20 June, 5 July and 20 July with ten cultivars. Strong interaction between cultivar and sowing date was detected in number of branch, node and pod. No significant interaction was observed in 100-grain weight and yield, and this might be resulted from similar yield reduction pattern. Daepung, Taekwang and Uram recorded the highest yield when planted late, proving that these three cultivars are the most adaptable to delayed planting. Delayed planting results in higher sugar content, especially stachyose and raffinose which have detrimental effect on human health by causing flatulence and diarrhea. Protein content was decreased and oil content was increased in July 20 planting. Decrease in protein content was the largest in Hwangeumol and maintained high in Saedanbaek. This information will provide a guideline when select appropriate soybean cultivar in delayed planting to maintain yield and seed quality.