Background: The International Classification of Functioning, Disability, and Health-Child and Youth version (ICF-CY) is designed to record the characteristics of developing children and examine the influence of a child’s environment on their health. Objects: This study was designed to determine the relationship between the clinically extracted ICF-CY items and The Pediatric Evaluation of Disability Inventory (PEDI) and Gross Motor Function Measure (GMFM) items. Methods: Thirty patients (17 males and 13 females) who were hospitalized in a pediatric and youth patient unit of a rehabilitation hospital were included in the study. Four health professionals (two physical therapists and two occupational therapists) working independently linked the PEDI and GMFM-66 items to the activity and participation domains of the ICF-CY. Results: There were strong negative correlations between the ICF-CY subdomains and the PEDI subdomains (r = 0.76–0.95; p < 0.05). There were positive strong correlations between the ICF-CY subdomains and the GMFM-66 (r = 0.76–0.95; p < 0.05). Conclusion: The extracted ICF codes were a valid tool for evaluating the mobility and selfcare conditions of cerebral palsy in the pediatric rehabilitation area.

This study aimed to investigate the function of the constitutively activating mutation D540G on eel FSHR activity by in vitro functional studies. Site-directed mutagenesis was carried out to generate the D-to-G mutation at position 540 of the pcDNA3-eel FSHR construct. Vectors expressing either wild type or mutant receptor were transfected into Chinese hamster ovary (CHO-K1) cells. The functional characteristics of both the wild type and mutant receptors were analyzed by a cAMP assay. cAMP accumulation was highly increased in cells transfected with the D540G mutant receptor in a dose-dependent manner. Of note, basal cAMP levels were remarkably increased (~13.1-fold) with expression of this mutant when compared to wild type receptor. These findings suggest that the D540G mutation in the eel FSHR may contribute to ovulation during eel sex maturation as well as play a pivotal role in inducing FSHR activity.

플랜트의 유황 저장 탱크는 강재로 구성되며, 탱크 저면은 앵커볼트에 의해 Ring Wall 형상의 콘크리트 기초와 연결된다. 탱크 내 유황이 내부 열원에 의해 고온상태를 유지하기 때문에, 유황 저장 탱크는 상온의 유체를 저장하는 다른 탱크에 비해 큰 체적팽창을 겪게 된다. 일반적으로 탱크 기초의 구조설계는 기초의 내외부의 온도차를 하중으로 적용한 구조해석이 수행 되는데, 이 방법은 탱크의 열팽창 특성이 앵커볼트에 의해 집중하중 형태로 콘크리트 기초에 전달되는 현상을 고려할 수 없 다. 이는 온도하중의 영향을 과소평가하게 되며, 앵커볼트에 인접한 콘크리트의 균열을 야기한다. 본 연구는 앵커볼트에 의 한 온도 하중전달 메커니즘을 고려한 하중 평가식을 제안함으로써, 콘크리트 기초에 작용하는 하중을 보다 합리적으로 결정 하고자 한다. 이를 위해 탱크 바닥판과 앵커볼트가 포함된 유한요소모델을 이용해 앵커볼트 개수 증감에 따른 온도하중의 변화를 분석하였으며, 분석결과를 이론해와 결합해 앵커볼트에 의해 전달되는 하중을 평가할 수 있는 명시적인 형태로 해를 제시하였다. 제안된 식의 유효성을 확인하기 위해 실제 플랜트 현장의 유황 저장 탱크 설계에 적용하였으며, 실무적으로 사 용 가능함을 보였다.

In all mammalian species, progesterone is essential in the preparation for and maintenance of pregnancy, if it occurs. Progesterone primes the endometrium for possible implantation and inhibits uterine contraction until birth. Aldo-keto reductases (AKRs) belong to a superfamily of NADPH-dependent reductases that act on a wide range of substrates, including simple carbohydrates, steroid hormones, and endogenous prostaglandins. 20-alpha hydroxysteroid dehydrogenase (20α-HSD; EC.1.1.1.149) enzyme belongs to the family of aldo-keto reductases. 20α-HSD predominantly converts progesterone into its biologically inactive form 20α-hydroxyprogesterone (20α -OHP), and plays a crucial role in the termination of pregnancy and initiation of parturition. In addition, the activity of 20α-HSD during the luteal phase is known to be inhibited by prolactin. We have been reporting on the molecular characterizations of placental and ovarian 20α-HSD in the bovine, pig, deer and monkey. In this study, we focused on the 20α-HSD expression in testis(6, 9, 12, 18 and 21 days after birth) of miniature pig. The protein expression and localization were detected by Western blotting and Immunohistochemical analysis. 20α-HSD protein was detected at molecular weight of 37-kDa by Western blot analysis. Also the RNA expression were detected by Reverse Transcription-PCR and quantification PCR. Additionally, We are going to analysis the function and role of 20α-HSD in the pig testis.

The glycoprotein hormone family consists of follicle-stimulating hormone (FSH; GTH1), luteinizing hormone (LH; GTH2), and thyroid-stimulating hormone (TSH), which are secreted by the pituitary gland in all mammalian species, and chorionic gonadotropin, which is secreted by placental trophoblast cells in primates and equids. These hormones consist of non-covalently associated α-, β- subunits. Within a species, the amino acid sequence of α-subunit is identical across all glycoprotein hormones and is encoded by a single gene. The αβ dimer is the active form of the hormone, and biological specificity is conferred by the β-subunit. Both of α and β subunit of eel FSH has two N-glycosylation sites (α-subunit: Asn56 and Asn79; β -subunit: Asn5 and Asn22, respectively). In the present study, we constructed deglycosylated mutants at single and double sites in each subunits of eel FSH for identification of Asn linked oligosaccharides' biological role. Mutant cDANs were cloned into pcDNA3 expression vector and transiently transfected into CHO suspension cells. The quantity of rec-eelFSHs were quantified by sandwich ELISA system, using monoclonal antibodies produced in our lab. The wild type rec-FSH protein was detected at the predicted molecular weight of 34 kDa by western blot. The molecular weight of deglycosylated mutants at single site decreased with about 4 kDa and of mutants at double sites decreased with 8 kDa. After PNGase treatment in the rec-eel FSH proteins, molecular weight also decreased to 7-8 kDa. We generated stably parental cell lines, engineered to express a β-arrestin 2EA fusion protein, expressing eel FSHR and C-terminal deleted mutant. 2 out of 5 receptor cells each were selected by G-418 and we tested these cell lines in a receptor functionality using PathHunter β arrestin assay (DiscoverX). Follicle stimulating hormone acts through binding to its specific receptor. Binding of ligand to the receptor activates the adenosine 3',5'-cyclic monophosphate (cAMP) pathway (McFarland et al., 1989; Ji and Ji, 1991a; Rose, 1998) and the inositol 1phosphate (IP1) the second messenger systems. After stimulation of eelFSH receptor stably transfected Parental CHO cells with FSH wild type and mutant hormones as a ligand, production of cAMP and IP-1 were evaluated (Cisbio). cAMP IC-50 values by eelFSHwt; αΔ56; αΔ79; αΔ56_79; and βΔ5 were 33.1; 1154.7; 22; 410 and 311.9 ng/ml, respectively. IP-1 IC-50 values by eelFSHwt; αΔ56; αΔ79; αΔ56_79 and βΔ5 were 6.8; 7.1; 4.4; 3.8 and 10.2 ng/ml, respectively too. The cAMP activation was greatly decreased in the αΔ56αmutant. Thus, the site of α56 oligosaccharide in the eel plays an pivotal role for the cAMP stimulation using eel FSH receptor cell lines. In the IP-one assay, the activity in the αΔ56 and βΔ5 mutants was a little decreased than the wt. The biological roles of N-linked oligosaccharides in GPCR internalization are going to be estimated by measuring β arrestin recruitment system.

The glycoprotein hormone family consists of luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone, which are secreted by the pituitary gland in all mammalian species, and choriogonadotropin (CG), which is secreted by the placenta in primates and equids. The hormones are composed of a common α subunit and a hormone specific β subunit which are non-covalently associated. Recent advances in biotechnology, particularly in the production of recombinant proteins, have provided opportunities to produce sufficient quantities of recombinant fish GTHs using various expression hosts. Japanese eel Anguilla japonica is one of the most important fish species being aquacultured in Japan but is hampered from the fact that this species does not reproduce in captivity. Artificial induction of gonadal maturation has been successful by administration of pituitary extracts or human chorionic gonadotropin, but the understanding the regulatory mechanism of gonadal development moderated by follicle stimulating hormone (FSH) and luteinizing hormone (LH) remains elusive due to lack of suitable amounts of eel gonadotropins (GTHs). In the present study, we produced tethered rec-eel LH and deglycosylated mutants (56, 79 and 56-79 of α subunit; 10 of β-subunit) of Asn-linked oligosaccharides in CHO suspension cells. Luteinizing hormone acts through binding to its specific receptor. Binding of ligand to the receptor activates the adenosine 3',5'-cyclic monophosphate (cAMP) pathway (McFarland et al., 1989; Ji and Ji, 1991a; Rose, 1998) and the inositol 1 phosphate (IP1) secondary messenger systems. After stimulation of eelLH/CG receptor transfected CHO cells with rec-LH wild type (wt) and mutant hormones as a ligand, production of cAMP and IP-1 were evaluated (Cisbio). cAMP IC-50 values by rec-eelLH wt; αΔ56; αΔ79; αΔ56_79 and βΔ10 were 606.2; 374.9; 100.3; 14.2 and 210.9 ng/ml, respectively. IP-1 IC-50 values by rec-eelLH wt; αΔ56; αΔ79; αΔ56.79 and βΔ10 were 28.3; 16.04; 4.3; 2.1 and 3.6ng/ml, respectively too. As seen in both of the second messenger production, general stimulatory pattern is analogous. cAMP and IP-1 stimulation by wild type and αΔ56, as well as αΔ79 and βΔ10 were approximate, but the stimulating effect of double mutant (αΔ56_ 79) was drastically higher. According to the data, deglycosylated eelLH may bind to the receptor with high affinity and cAMP production is gradually increased. Furthermore, receptor activation by tethered rec-eel mutant ligands (FreeStyle CHO-MAX Expression System) will be evaluated with β arrestin recruitment and GPCR internalization for N-linked oligosaccharides’ biological role in activation of eelLH/CGR.

Equine chorionic gonadotropin (eCG) is a unique molecule that elicits the response characteristics of both follicle- stimulating hormone (FSH) and luteinizing hormone (LH) in other species. Previous studies from this laboratory had demonstrated that recombinant eCG (rec-eCG) from Chinese hamster ovary (CHO-K1) cells exhibited both FSH- and LH-like activity in rat granulosa and Leydig cells. In this study, we analyzed receptor internalization through rec-eCGs, wild type eCG (eCGβ/α) and mutant eCG (eCGβ/αΔ56) with an N-linked oligosaccharide at Asn56 of the α-subunit. Both the rec-eCGs were obtained from CHO-K1 cells. The agonist activation of receptors was analyzed by measuring stimulation time and concentrations of rec-eCGs. Internalization values in the stably selected rat follicle-stimulating hormone receptor (rFSHR) and rat luteinizing/ chorionic gonadotropin receptor (rLH/CGR) were highest at 50 min after stimulation with 10 ng of rec-eCGβ/α. The dose-dependent response was highest when 10 ng of rec-eCGβ/α was used. The deglycosylated eCGβ/αΔ56 mutant did not enhance the agonist-stimulated internalization. We concluded that the state of activation of rFSHR and rLH/CGR could be modulated through agonist-stimulated internalization. Our results suggested that the eLH/CGRs are mostly internalized within 60 min by agonist-stimulation by rec-eCG. We also suggested that the lack of responsiveness of the deglycosylated eCGβ/ αΔ56 was likely because the site of glycosylation played a pivotal role in agonist-stimulated internalization in cells expressing rFSHR and rLH/CGR.

시뮬레이터는 다양한 형태와 기능을 갖춘 여러 장치들로 구성되어 있으며, 이 장치들이 네트워크로 연동된 복잡한 구조를 이루고 있다. 이러한 이유로 시뮬레이터 개발 및 유지보수 과정에 많은 시간과 비용이 소요된다. 시뮬레이터의 성공적인 개발을 위해서는 관련 전문 가들이 협력하고 업무를 분담하여 병렬적으로 수행하는 것이 이상적이지만 업무의 상호 의존성이 이를 어렵게 한다. 본 논문에서는 시뮬레이 터 개발 업무를 알고리즘 구현과 이를 제외한 시스템 구현으로 나누어 두 업무의 상호 의존성을 낮추고 전문가를 지원하는 방안을 검토하였 다. 특히 알고리즘 구현을 담당하는 도메인 전문가의 요구 사항을 분석함으로써 시뮬레이터 개발 지원 도구를 설계하고, 이를 활용한 시뮬레 이터 개발 절차를 제안하였다. 또한 도메인 전문가의 알고리즘 개발 지원과 유연한 데이터 관리를 위해 데이터셋 개념을 도입하였고, 시뮬레 이터 장비들이 유연하게 재구성될 수 있도록 네트워크 구조를 설계 하였다. 시뮬레이터 개발 지원 도구를 통해서 도메인 전문가는 알고리즘 개발에 전념할 수 있고, 효율적인 협업이 가능할 것으로 기대된다. 또한 개발 절차가 체계화 되고 더 명확해지기 때문에 개발 계획 및 관리가 용이해질 것으로 예상 된다.

This study were to simulate major criteria air pollutants and estimate regional source-receptor relationship using air quality prediction model (TAPM ; The Air Pollution Model) in the Seoul Metropolitan area. Source-receptor relationship was estimated by contribution of each region to other regions and region itself through dividing the Seoul metropolitan area into five regions. According to administrative boundary, region Ⅰ and region Ⅱ were Seoul and Incheon in order. Gyeonggi was divided into three regions by directions like southern(region Ⅲ), northern(Ⅳ) and eastern(Ⅴ) area. Gridded emissions (1km×1km) by Clean Air Pollicy Support System (CAPSS) of National Institute of Environmental Research (NIER) was prepared for TAPM simulation. The operational weather prediction system, Regional Data Assimilation and Prediction System (RDAPS) operated by the Korean Meteorology Administration (KMA) was used for the regional weather forecasting with 30km grid resolution. Modeling period was 5 continuous days for each season with non-precipitation . The results showed that region Ⅰ was the most air-polluted area and it was 3～4 times more polluted region than other regions for NO2, SO2 and PM10. Contributions of SO2 NO2 and PM10 to region Ⅰ, Ⅱ and Ⅲ were more than 50 percent for their own sources. However region Ⅳ and Ⅴ were mostly affected by sources of region Ⅰ, Ⅱ and Ⅲ. When emissions of all regions were assumed to reduce 10 and 20 percent separately, air pollution of each region was reduced linearly and the contributions of reduction scenario were similar to those of base case. As input emissions were reduced according to different ratio - region Ⅰ 40 percent, region Ⅱ and Ⅲ 20 percent, region Ⅳ and Ⅴ 10 percent, air pollutions of region Ⅰ and Ⅲ were decreased remarkably. The contributions to regionⅠ, Ⅱ, Ⅲ were also reduced for their own sources. However, region Ⅰ, Ⅱ and Ⅲ affected more regions Ⅳ and Ⅴ. Shortly, graded reduction of emission could be more effective to control air pollution in emission imbalanced area.

Aircraft Measurements of gaseous pollutants(SO2, NOx and O3) in the Yellow Sea area, were carried out on 1997-2007. Main measurement site in 124°-127°E, 35°-37°N (in the Yellow Sea), have been done along the paths classified vertically and zonally. To understand how the air stream affects Long-range transboundary pollutants in Northease Asia (LTP), the tracks of pollutants in northeast Asia have been analyzed by dividing into 6 different regions(regions I-V and L). Compared with Korea's local sources and western north Pacific influenced by the Yellow Sea, when the air stream from region Ⅱ is dominant, the SO2 concentrations are 3-6times higher. In region Ⅱ and Ⅲ, SO2 concentrations are represented highest at 25.0 and 14.7 ppb, respectively. However, in other regions, SO2 concentration was recorderd the highest at 1.1-3.8 ppb, which is 7-15% higher then the highest one over the region Ⅱ and Ⅲ. During 1997-2007, the mean amount of incoming pollutants is 0.162 ton/km·hr exceeding about 5-times mean amount of outgoing pollutants over the West Sea. During the observed period, the amount of incoming and outgoing SO2 over the Yellow Sea is the highest in winter, at 0.224 ton/(km·hr) and 0.120 ton/(km·hr), respectively.