The estrogen-mediated effect of mesenchymal stem cells (MSCs) is a highly critical factor for the clinical application of MSCs. However, the present study is conducted on MSCs derived from adult donors, which have different physiological status with steroid hormonal changes. Therefore, we explores the important role of 17β-estradiol (E2) in MSCs derived from female and male newborn piglets (NF- and NM-pBMSCs), which are non-sexually matured donors with steroid hormones. The results revealed that in vitro treatment of MSCs with E2 improved cell proliferation, but the rates varied according to the gender of the newborn donors. Following in vitro treatment of newborn MSCs with E2, mRNA levels of Oct3/4 and Sox2 increased in both genders of MSCs and they may be correlated with both estrogen receptor α (ERα) and ERβ in NF-pBMSCs, but NM-pBMSCs were only correlated with ERα. Moreover, E2-treated NF-pBMSCs decreased in β-galactosidase activity but no influence on NM-pBMSCs. In E2-mediated differentiation capacity, E2 induced an increase in the osteogenic and chondrogenic abilities of both pBMSCs, but adipogenic ability may increased only in NF-pBMSCs. These results demonstrate that E2 could affect both genders of newborn donor-derived MSCs, but the regulatory role of E2 varies depending on gender-dependent characteristics even though the original newborn donors had not been affected by functional steroid hormones.

Choline is an amine essential to maintenance of good health. Here, we modified the ion chromatographic method for measuring choline described by Laikhtman & Rohrer and validated by the ICH guideline and applied it to monitoring of choline content in infant formula in the Republic of Korea. The Certified Reference Materials (CRM), NIST 1849a, were used for validation of the modified method. The parameters of validation were linearity, range, specificity, accuracy, precision, LOD, LOQ and recovery. The linearity, R2, was above 0.999 and the analytical range was 2.5 to 50 μg/mL. The specificity was confirmed by the retention time (RT) and positive tests with calibration standard and CRM. The accuracy, precision and recovery values were 99.6 ± 2.41 %, 2.42 % and 90.8 - 92.8 %. The LOD and LOQ values were 0.03 mg/kg and 0.17 mg/kg, respectively. Five livestock products inspection agencies voluntarily participated in the collaborative study. The result, HORRAT value was 0.8 which was within the reference value 0.5 – 2.0. The validated method was applied to the analysis of choline in infant formula in the Republic of Korea. The results also conformed to 'The Standard for Livestock Products Labeling'.

Vitamin K1 is a fat-soluble vitamin essential for human health. We used the Association of Official Analytical Chemists(AOAC) method 999.15, validated by the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH) Guideline, to monitor vitamin K1 content in infant formula in the Republic of Korea. The Certified Reference Materials (CRM), NIST 1849a, were used for validation of the test method. The parameters of validation were linearity, range, specificity, accuracy, precision, LOD, LOQ and recovery. The linearity, R2 was above 0.999 and the analytical range was 0.001 to 0.22 μg/mL. The specificity was confirmed by the retention time (RT) and positive/negative sample test. The accuracy, precision and recovery values were 101.13 ± 0.13 %, 3.15 % and 81.60 % - 99.78 %. The LOD and LOQ values were 0.582 ng/mL and 1.76 ng/mL, respectively. Five livestock products inspection agencies voluntarily participated in the collaborative study. The HORRAT value was 0.6, which was within the reference value 0.5 – 2.0. The validated test method was applied for the monitoring of vitamin K1 in infant formula in the Republic of Korea. The results also conformed to 'The Standard for Livestock Products of Processing and Ingredient Specifications' and 'The Standard for Livestock Products Labeling'.

우리나라는 비약적인 산업기술발전을 통해 선진국 진입을 눈앞에 두고 있다. 하지만 선진국 진입에 있어 크게 세 가지의 걸림돌이 있다. 첫째로는 세계시장 진입의 장벽, 둘째로 제조물책임법(PL법) 대응방안 미흡, 셋째로는 고객신뢰 및 자원의 한계 이다. 이러한 한계점으로 인하여 신뢰성경영시스템의 필요성이 대두되고 있으며 국내의 신뢰성경영시스템의 활성화는 필수 요소로 다가오고 있다. 본 논문에서는 신뢰성경영시스템의 활성화와 이를 위한 지표개발의 필요성 및 개발 방향성을 제시해보고자 한다.

IT기술의 급격한 발달과 컴퓨터 보급의 일반화로 일상생활뿐만 아니라 생산과 판매 등의 산업현장에 있어서도 컴퓨터가 활용되지 않는 분야가 없다. IT기술의 발달과 결부된 소프트웨어분야의 지속적인 기술 개발은 IT기술의 이용효율성을 증대시켜서 컴퓨터의 이용범위 확대와 이용률을 제고 시키고 있다. 컴퓨터의 이용률이 높아지면서 다양한 분야에서의 소프트웨어 개발에 대한 필요성이 제기되었고 소비자들은 원하는 소프트웨어 선택의 폭을 넓히고 있다. IT분야는 바야흐로 소프트웨어의 품질이 IT자체의 제품선택에 영향을 미치는 중요한 관건이 되는 시기로 접어들게 되었다. 본 연구는 소프트웨어의 품질을 평가하기 위해 제품을 측정하는 국제적인 표준에 대한 개선방안의 제시에 중점을 두었다. 이를 위하여 앞으로 제정될 ISO / IEC 25000에 대해 소개하고, 한걸음 더 나아가서 앞으로 소프트웨어의 품질을 효과적으로 관리할 수 있는 방법에 대한 기초자료를 이론적으로 제시하고자 한다.