벗초파리기생벌인 A. japonica의 벗초파리 유충의 발육단계에 따른 기생특성과 기생당한 유충과 번데기에서 형태적인 차이를 조사하였다. 또한 A. japonica의 우화기간이 벗초파리 우화일수 보다 더 소요되는 것을 확인하였다. A. japonica는 벗초파리 유충에 효과적인 기생 및 살충 효과를 나타내었으며 벗초파리 방제를 위한 천적으로 활용이 가능할 것으로 보인다.
Mealworm(Tenebrio molitor) was added to the feed to improve the health of dogs. As the amount of mealworm powder added to dog feed increased from 2.5 to 7.5%, the contents of crude protein, crude fat, crude fiber and crude ash increased. In the old dogs, creatinine and BUN related to nephropathy, triglyceride related to hypertension, calcium related to osteoporosis, globulin related to immunity, and ALT related to hepatic function were in the normal range. Particularly, ALT showed a tendency to increase overall, but the numerical increase tended to decrease as the amount of mealworm powder added. In the young dogs, all investigative items such as BUN and creatine were in the normal range and ALT tended to decrease at 2.5～5.0% treatment of mealworm. As a result, it was shown that adding 2.5～5.0% of mealworm powder to feed effectively improved the hepatic function of old and young dogs.
This study was carried out to confirm the predatory and developmental features of N. stenoferus. We determine the host range of N. stenoferus. As a result, it was confirmed that Aphis gssypii, Myzus persicae, Planococcus citri, and Frankliniella occidentalis. N. stenoferus is thought to be able to feed on other micro pests. The test for a developmental period of N. stenoferus at 25℃ showed that the egg period was about 10 days. The nymphal period was about 18 days. Each nymphal period from 1st instar to 3rd instar nymphs was about 3 days. And the nymphal period of 4th and 5th were about 3.5 and 6 days, respectively. The female adult laid eggs in stem tissue or on leaves, and sometimes on the soaked cotton for water supply.
Drosophila suzukii (Matsumura) is one of the most destructive pest of thin-skinned fruits such as blueberry, strawberry, raspberry, and cherry. We tested effect of fruit damage on the infestation of D. suzukii by using partially peeled blueberry fruits in assays of behavioral choice and development. In choice test, D. suzukii preferred partially peeled blueberry fruit than normal. But in development test, we did not found difference in adult emergence rate between normal and partially peeled blueberry fruit.
Childrena zenobia is very hopeful as an industrial insect for its beautiful color and long life span. This study was conducted to establish the mass rearing methods of C. zenobia to develop it as an industrial insect.
C. zenobia appeared in the high mountains of about 1,000m above sea level at Pocheon and Gapyoung during high temperature season from July to August, and in the low mountains of about 400m above sea level at Yeoncheon on September. The first oviposition time after mating took 27.8±9.0 days and the number of eggs per female was 180. The life span of adult was 39.3±20.2 days in male and 59.0±18.7 days in female. The hatchability of F1 generation showed high rate over 80% at 20∼30℃, while the rate of F5 generation was very low under 5%. The proper food for rearing the larva was Viola papilionacea. The developmental period from 1st larva to adult was 63.6±5.1 days and the hatching time was 8.7±0.9 days at 20℃ and 48.0±3.3 days and the hatching time was 8.1±1.2 days at 25℃.
These results seemed to be very suggestive that C. zenobia could be massively reared in the condition of indoor rearing room resulting in the utilization of it as an industrial insect for various regional festivals.
Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end, the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.
Baculum elongatum has a long, thin shape that resembles a twig, propagates by parthenogenesis, changes body color, and drops eggs, all of which suggests its potential in the commercial market, especially as an educational pet insect. This study was carried out to determine the best hatching environment and an alternative or artificial diet for rearing B. elongatum to be developed as a commercial and educational pet insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was 42.2 ± 22.7 days and the number of eggs per female was 109.5 ± 70.5 eggs. The hatch rate was 73.3% at 25°C and 66.7% after low temperature treatment (8°C for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet. To rear nymphs and adults of B. elongatum, natural diet such as acacia, white oak, chestnut, or bush clover leaves, or an alternative diet such as clover or an artificial diet can be used.