본 연구는 장미 ‘Bubble Gum’의 절화수명을 연장시키기 위하여 미산성 차아염소산수(Slightly acidic hypochlorous water, HOCl, pH 6.26)의 처리효과와 절화수명 연장제로써 의 가능성을 구명하고자 수행되었다. 침지처리는 30μL･L-1의 미산성 차아염소산수를 0분, 0.5분, 1분, 3분, 5분간 침지처 리 후 수돗물(Tap water, control)에 꽂아 두었다. 대조구의 절화수명은 8.7일인데 비해 5분 침치처리 시 10.8일로 대조 구보다 2.1일 수명을 연장시켰다. 상대생체중은 모든 처리구 에서 2일까지 증가하였고, 수분흡수율은 모든 처리구에서 6일 까지 증가하다가 감소하는 추세를 보였다. 화색 변화율인 ΔE 값은 3분(5.25), 5분(6.48), 30초(6.65), 1분(6.79) 침지처리 에서 대조구보다 낮았으며, 상대 화폭 증가율은 5분 침지 처 리구가 145%로 화폭의 증가율이 가장 컸고, 엽록소함량은 처 리 간 차이가 없었다. 결론적으로 절화장미 ‘Bubble Gum’의 절화수명은 미산성 차아염소산수의 5분 침지 처리가 효과적 으로 연장시켰다.

본 연구는 장미 ‘Bubble Gum’에 대한 수확 후 LED 광 환경 과 살균제 Azoxystrobin 보존용액 처리 시 절화품질에 미치는 영향을 구명하고자 수행되었다. 광은 백색 LED와 유색 LED (red:blue=5:1)로 처리하였고, 보존용액은 수돗물(tap water, TW)과 Azoxystrobin 0.05 mL･L-1를 처리하였다. 백색 LED처 리의 절화수명은 TW, Azoxystrobin처리구 각각 9.6일, 9.7일 로 LED 광과 보존용액 처리 간의 유의차가 없었다. 유색 LED 처리의 절화수명은 TW처리구가 13.6일, Azoxystrobin처리구 가 9.8일로, 유색 LED 처리구가 대조구(백색 LED + TW)에 비해 절화수명을 4일 연장시켰다. 절화수명 종료 증상은 LED 광 조건과 관계없이 Azoxystrobin처리구는 꽃잎 위조와 청변 화 증상을 감소시켰다. 모든 처리구에서 상대생체중과 수분흡 수율은 각각 처리 후 2일, 4일까지 증가하다 감소하는 경향을 보였다. 수확 직후 대비 처리 6일 후 화색 변화율과 잎의 엽록 소 함량은 수확 직후와 모든 처리구에서 차이가 없었으며, 화 폭증가율도 처리 간 차이가 없었다. 결론적으로 백색 LED + Azoxystrobin 보존용액처리는 절화장미의 꽃잎 위조와 청변화 증상을 감소시키지만, 절화수명 연장효과가 없었으며, 적색 +청색 LED 처리는 장미의 절화수명 연장효과에 효과적이었다.

This study aims to investigate the proper mixing treatment concentration of ozone (O3) and sucrose to preserve and extend the vase life of the cut rose flowers ‘Dominica’. The vase solution was prepared using tap water, 3% sucrose, ozone 5.5 mg L-1, and 3% sucrose with ozone 5.5 mg L-1. The vase life was the highest in the tap water and ozone treatments at 16.3 and 16.1 days, respectively. The vase life of ozone with sucrose treatment was 6.9 days, which was 9.4 days lower than that of the control. Compared to a single treatment, the vase life termination symptoms for ozone with sucrose treatment decreased petal wilting and increased bent necks. Relative fresh weight and vase solution uptake increased up to 4 days after treatment and decreased from 2 days before vase life termination. The rate of change in petal color was high in L*, a*, and b* for the sucrose treatment than after harvest, and low for the ozone treatments. The maximum relative flower size increase rates after treatment were 195% in the control, 186% in the sucrose treatment, 171% in the ozone treatment, and 155% in the ozone with sucrose treatment.

Background : Panax ginseng C. A. Meyer is a slow-growing perennial herb that is cultivated in shading condition. Climate change occur around the world that make a lot of problem such as damage of high temperature, drought, salinity and disease. The problems lower the ginseng productivity that cause income reduction of farmers. To achieve stable ginseng production, development of elite varieities resistant to abiotic and biotic stresses is consistently required. It is very time consuming process in order to develop new ginseng varieties because ginseng flowers after 3 years of growth. So, early selection system of elite line must be established. This study was conducted to develope efficient ginseng breeding techniques for early identification of heat or salinity resistance. Methods and Results : Ginseng petioles was soaked in mixed salts solution consisting of KNO3, KH2PO4, MgSO4․H2O in order to test resistant or susceptible salinity. The degree of resistance was quantified according to damage size. Also, ginseng lines transplanted in pot were treated 46℃ for 1 hour and then chlorophyll fluorescence reaction were measured in order to test resistant or susceptible high-temperature. The measured values such as Fm/Fo, Fv/Fm, Rfd were differentiated between resistant and susceptible line. Conclusion : Several lines showed that they are resistance to high temperature or salinity. The selected lines will be utilized for parents to develop new varieties.

Background : Water uptake and flow across cellular membranes is a fundamental requirement for plant growth and development, and plant water status is important not only for plant growth under favorable conditions but also for ability of a plant to tolerate adverse environmental conditions. Thus identification of plasma membrane water channel genes (aquaporins) in ginseng provides extensive information for functional studies and the development of markers for salinity stress tolerance. Methods and Results : For salinity treatment, the plants were grown for 4 weeks in culture medium gelled with 0.8% Phytoagar, and the old media were replaced with the fresh medium containing NaCl at 0, 50, 100, 200 and 400 mM, respectively. The samples for stress treated and non-stressed plants were collected from 6h to 72h, and frozen immediately into liquid nitrogen. According to the sequence information from the assembled transcripts, four primer pairs were designed from the aquaporin gene regions. In order to determine the pattern of aquaporins expression in ginseng seedlings to salinity stress, we conducted semi-quantitative RT-PCR. Conclusion : A tonoplast intrinsic protein 1 (TIP1)-type aquaporin is not only believed to be essential for plant life, but also to be beneficial for growth under salinity stress. Therefore, a deeper understanding of aquaporin genes in ginseng will be essential for crop improvement, which could help us to understand the molecular genetic basis for the ginseng genetic improvement and also provide the functional genetic resources for selective breeding and transgenic research.

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.

Background : Due to immature development of embryo in ripened berries, dehiscence process is required for the proper germination of ginseng seeds. Such process involves the preparation of the container with alternating layers of seed and moist sand. In order to make sand fully moist, water sprayer has been usually used by farmers, which is labor intensive, time consuming and causing uneven sand moisture. Methods and Results : In this study, we investigated the effects of different stratification methods on dehiscence ratio of ginseng seeds. Ginseng seeds were stratified for 90 days in a total of 12 different treatments and the dehiscence ratios were compared; drainage methods, drainage time, the ratio between ginseng seeds and sand, and etc. Seed stratification process was performed according to the guideline of ginseng GAP. One thousand ginseng seeds were used for each treatment. It was found that the average of dehiscence of the 12 treatments was 84.6 %. The highest dehiscence ratio (90.3 %) was observed in the seeds that were treated with water soaking, immediately followed by drainage. Higher ratio was also observed in the seeds that were soaked for 60 min, followed by drainage. Therefore, our findings indicate that ginseng seeds soaked in water less than 60 min could dehisce more efficiently than traditional method. Conclusion : Our study demonstrated that ginseng seeds that are subjected to water soaking and then drainage showed better ration of dehiscence. This method will eventually decrease the time and labor used for seed stratification.

Background : This study was conducted to determine the impact of temperature elevated and the effect of transplanting times based on climate change scenario on growth of 2-year-old korean ginseng (Panax ginseng C. A. Meyer.) in temperature gradient chambers (TGC). Methods and Results : As a plant materials, ‘Yunpoong’ was cultivated in TGC at ambient temperature(Amb), Amb+2℃, Amb+4℃ and Amb+6℃ respectively. Ginseng was also transplanted on March 29, April 12 and 26 respectively. Investigation on characteristic of aerial parts were carried out on 28, 56, 84 and 112 days after transplanting and characteristic of roots were conducted on October 19. As transplanting time was faster and temperature was higher, the growth of aerial parts were increased. Compared with those of ginseng transplanted on March 29 with Amb, the root weight which tend to decrease depending on late transplanting time and high temperature decreased about 11.1%, 35.4% and 42.4% in Amb+ 2℃, Amb+4℃ and Amb+6℃ respectively. Ginseng transplanted on April 12 and 26 decreased about 20.9%, 33.9% respectively. Conclusion : Consequently, the more transplanting time extend, the more quantity increased in all temperature treatment. So, it is possible to increase in quantity to advance transplanting time although high temperature will be caused by the climate change.

Background : When ginseng seeds were gathered, the seeds were unripe. To grow immature embryo definitely, special treatment called dehiscence must be performed. Even though dehiscence is completed, most ginseng seeds are on enforced dormancy. The breaking seed dormancy is generally achieved using cold treatment. Also it is reported that gibberellin treatment can replace the treatment. It is very time consuming process in order to develop new ginseng cultivar because ginseng flowers after 3 years of growth. To shorten the ginseng breeding period, it is necessary to establish fast generation progress. Therefore, this study examined the possibility of breaking seed dormancy of ginseng using GA3 treatment and alternating temperature. Methods and Results : Seeds were obtained from local variety fruit which is not inbred. Gibberellin of 100 ppm was treated at seeds for 24 hours. Fixed cold condition was treated on both –2℃ and 2℃. Alternating cold condition was treated on 2℃ and then –2℃, finally 2℃. Fixed and alternating temperature was continued for 15, 30, 45, 60, 90 days that 15 days of alternating temperature is first 2℃ for 5days and then -2℃ for 5days, finally 2℃ for 5days. The other treatment periods such as 30, 45, 60, 90 days mean 10, 15, 20, 30 days respectively. Each of 48 seeds were sowed on tray in greenhouse at 3 replication. Experimental plot was completely randomized. Conclusion : Seeds untreated with GA3 were germinated little and there is no difference between 2℃ and –2℃. Alternating temperature until 60days made no difference with fixed temperature but germination rate increased up to 70.8% when seeds were treated for 90days. Germination of seeds treated with GA3 is much higher than untreated seeds especially combined with alternating temperature.