A total of 447 accessions consisting of seven Brassica spp.; Brassica carinata (34), B. juncea (199), B. rapa subsp. dichotoma (18), B. rapa. subsp. oleifera (14), B. rapa subsp. rapa (36), B. rapa subsp. trilocularis (56) and B. alba subsp. alba (90) were studied for their morphological characters and fatty acid compositions. There was a wide variation for morphological traits, oil content and fatty acid composition among Brassica species. Seed number/silique and yield/plant were varied from 4.2 (B. alba) to 25.1 (B. rapa subsp. trilocularis) and from 170.7 g (B. rapa subsp. oleifera) to 351.9 g (B. juncea L. Czern.), respectively. Among Brassica species, B. rapa subsp. trilocularis exhibited the highest oil (29.2%), stearic (20.4%) and erucic acid (45.3%) content. B. carinata had the highest content of palmitic (5.2%), oleic (21.2%) and linolenic acid (11.1%). B. rapa subsp. dichotoma and B. rapa subsp. oleifera exhibited the highest content of linoleic (8.1%) and behenic (26.9%) acid, respectively. B. rapa subsp. trilocularis exhibited the highest (45.3%) erucic acid content and significant positive relationship was observed between oleic acid and linoleic acid. This variation of agronomic and fatty acid compositions in Brassica species can be utilized to develop new varieties.

Glucosinolates (GSLs), beneficial secondary metabolites for human health are abundantly present in radish vegetable. Radish is a member of Brassicaceae family and its seed, leaf and root contain very important GSLs. The objective of this study was to determine the variation of individual and total GSL contents in leaves and roots of 44 radish (Raphanus spp.) germplasm (26 R. sativus L., 3 R. raphanistrum, and 15 R. sativus L. var. raphanistroides Makino), and compare the GSL contents between leaves and roots among three Raphanus species. Thirteen GSLs were identified, being the glucoraphasatin (GRS) and glucobrassicin (GBS) the most abundant aliphatic and indolyl GSLs in both the leaves and roots. Variation in individual and total GSL contents was found among the germplasm of three Raphanus species. The GRS content was higher in roots than that of leaves in all three Raphanus species but the GBS content was higher in leaves than roots. GRS was represented 87.0%, 92.7% and 94.7% of the total GSL in roots of R. sativus L., R. raphanistrum and R. sativus L. var. raphanistroides (Makino) germplasm, respectively. Germplasm of R. raphanistrum exhibited the highest (average, 79.5 μmol/g dw) total GSL with a ranged from 62.7 to 92.9 μmol/g dw. The germplasm IT119288, Joseonmu and IT119262 from R. sativus L., RA 504 and K046542 from R. raphanistrum, and Gyeongju-2003-32 (G2003-32) and IT302373 from R. sativus L. var. raphanistroides (Makino) had high total GSL contents and these could be good candidates for developing the functional compounds-rich varieties in radish breeding program.

Identification of Fusarium oxysporum f. sp. raphani and investigation on fusarium wilt development by isolates and inoculation methods were conducted to establish a screening method for fusarium wilt-resistance in radish (Raphanus sativus L.) germplasm. Pathogenicity of F. oxysporum f. sp. raphani isolate, Heonggye-1 and Heonggye-2, to radish plants was confirmed by seedling test. Radish seedlings were inoculated by root-dipping and soil-drenching with or without root-wounding. For Heonggye-1 isolate, mean disease indexes were 4.13 and 3.91 by root-dipping and soil-drenching with root-wounding, respectively, but those were 1.87 and 1.88 without root-wounding. For Heonggye-2 isolate, mean disease indexes were 3.83 to 4.37 regardless of inoculation methods. Two-hundred sixty accessions of radish germplasm collected from 9 countries of Asia and Europe were evaluated for fusarium wilt-resistance by soil-drenching with root-wounding with Heonggye-2 isolate. Fifty-four resistant accessions with higher than 70% of the percentage of resistant seedlings in accession (PR) and lower than 20% of the percentage of susceptible seedlings in accession (PS) was found. Eighteen susceptible accessions with lower than 20% of PR and higher than 50% of PS were selected. These accessions could be used as breeding and research materials after re-evaluation of disease-resistance and characterization of agronomical traits.

Morphological variation between cultivated and weedy types of Perilla frutescens var. frutescens and P. frutescens var. crispa were studied in 327 germplasm by examining 17 morphological characters. The germplasm between the two varieties were varied for their qualitative and quantitative characters. The seed coat color of cultivated P. frutescens var. frutescens is commonly light brown and brown while deep brown color was observed in the weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The leaf size, cluster length, plant height, flower number per cluster and seed weight in cultivated P. frutescens var. frutescens were significantly (P<0.05) different from weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The cultivated P. frutescens var. frutescens exhibited significantly higher plant height (158.6 ㎝) compared to the weedy P. frutescens var. crispa (133.8 ㎝). Likewise, seed weight was significantly higher in cultivated (1.9 g) than in the weedy type of P. frutescens var. frutescens (1.6 g) and P. frutescens var. crispa (1.4 g). Principal component analysis (PCA) result showed that the first and second principal component cumulatively explained 86.6% of the total variation. The cultivated type P. frutescens var. frutescens and its weedy accessions were not clearly separated with P. frutescens var. crispa by PCA. Hence it requires the use of molecular markers for better understanding of their genetic diversity.

Lettuce is an important annual leafy vegetable and bitterness is its potent flavor character. Lettuce germplasm differ their phenotypic characters and sesquiterpene lactones (SLs) contents which are important for consumer’s acceptance. This study was carried out to evaluate the phenotypic characters and SLs contents in one hundred lettuce germplasm in Jeonju, Korea. Twenty-three agro-morphological (16 qualitative and 7 quantitative) traits and two SLs (lactucin and lactucopicrin) contents were studied in these germplasm. Germplasm exhibited the variation in qualitative and quantitative characters. Average plant weight was 423.9 g with a range from 116.0 to 905.0 g. Lactucin content was varied from 19.7 (IT 294226) to 194.4 ㎍/g (IT 294298) with an average concentration of 84.7 ㎍/g. Lactucopicrin ranged from 82.5 (IT 300134) to 2228.6 ㎍/g (IT 294210) with an average concentration of 586.3 ㎍/g. Total SLs content was ranged from 120.1 (IT 300134) to 2286.6 (IT 294210) ㎍/g with the average concentration of 671.0 ㎍/g. Significant (p≤0.05) differences were found between crisp head and butter head germplasm for lactucin, lactucopicrin and total SLs content. Crisp head germplasm revealed the highest average lactucin (112.9 ㎍/g), lactucopicrin (734.8 ㎍/g) and total SLs content (847.7 ㎍/g). Crisp head and leafy type germplasm exhibited more total SLs content (847.7 and 744.7 ㎍/g, respectively) than cos (524.9 ㎍/g) and butter head type (519.4 ㎍/g). Principal component analyses of the quantitative traits indicated that the first principal component axis accounted more than 91% of the total variation. This study revealed the ample genetic variation in the agro-morphological traits and SLs contents to support the selection for improved lettuce varieties.

Garlic (Allium sativum L.) has been used as both food and medicine in many cultures for thousands of years. Garlic cultivars are completely sterile and propagated through vegetative method. Collection of a large number of fertile accessions of these genus is needed to explore genetic variability. In order to investigate genetic variation among Allium species and its possibilities for direct cultivation in Korea, we characterized 12 accessions of A. longicuspis, flowering wild garlic which had collected from Central Asia, the main center of garlic diversity. Most of A. longicuspis accessions showed higher over-wintering and bolting rate, longer scape length and more number of bulbils than Korean landraces cultivar, Danyang and Euiseong, but A. longicuspis accessions exhibited smaller size of bulbs and bulbils. Most accessions of A. longicuspis had more number of cloves per bulb, except K229596 and K248824 than Korean landraces. All the accessions of A. longicuspis from Central Asia had complete bolters having many flowers and topsets in umbel. Further studies of A. longicuspis should focus on securing true seeds through removal of topsets and crosses among accessions to create the genetic variability.

Momordica charantia L. is a valuable food and medicinal plant of the gourd family (Cucurbitaceae) that is cultivated in tropical and subtropical regions of the world. Physicochemical properties of M. charantia based on cultivars, parts and growing stage were investigated. Crude protein contents of leaf were 27.5%, 26.9%, and 23.6% in native leaf (NL), cv. Erabu leaf (EL), and cv. Dragon leaf (DL), respectively. In particular, the crude protein content was the highest in leaves. The crude fat content was in the order of developmental stage 1 of cv. Erabu fruit (EF1) and developmental stage 1 of native fruit (NF1) with values of 4.0%, and 3.9%, respectively. There was also high amount of crude fiber in stem of all three cultivars. The crude ash content was in the order of cv. Erabu leaf, cv. Dragon leaf, and developmental stage 3 of native fruit (NF3) with values of 23.2%, 17.4%, and 13.6%, respectively. The major minerals found in M. charantia were K, Ca, and Mg. The potassium contents of developmental stage 3 of native fruit (NF3), developmental stage 3 of cv. Dragon fruit (DF3), cv. Dragon stem (DS), and developmental stage 2 of native fruit (NF2) were 498.37, 339.21, 314.30, and 307.34 ㎎ /100g, respectively, while the calcium contents were decreased of EL, DL, and NL with values of 513.45, 371.69, and 209.43 ㎎/100g, respectively. The calcium content was higher in leaves and stems than fruits. On the otherhand, the highest magnesium content was measured in EL (69.92 ㎎/100g). The highest contents of chlorophyll a, chlorophyll b, and total chlorophyll were found in NL (442.9 ㎎/100g dw), EL (759.6 ㎎/100g dw), and EL (1164.9 ㎎/100g dw), respectively. The vitamin C contents from developmental stage 2 of cv. Erabu fruit (EF2), NF3, developmental stage 3 of cv. Erabu on fruit (EF3), and NF2 were found with values of 2849.9, 2330.5, 1985.1, and 1844.5 ㎎/㎏, respectively, and found to be higher in Korean cultivar and Erabu fruit than in Dragon. The charantin contents of leaf were higher than the fruit found to be 547.71, 506.04 and 395.62 ㎍/g dw in DL, EL and EF2, respectively. According to the results, mineral contents, total chlorophyll and charantin contents of M. charantia were higher in the leaves (EL and DL) than the fruits. And, vitamin C content was the highest in the fruit (EF2 and EF3). Therefore, much more research needs to be undertaken to use of the leaves as well as fruits. The data showed that M. charantia can be considered a good source of nutrient for application in food system.

This study aimed to evaluate 105 tomato accessions conserved in National Agrobiodiversity Center regarding their resistance to Ralstonia solanacearum, a soil-borne vascular bacterium that causes lethal wilt diseases of a wide range of crops worldwide. All the accessions are Solanum lycopericum var. lycopersicum including cultivar or breeding lines. At the four leaf stage, the seedlings were inoculated by drenching the soil with the bacterial suspension concentrated of 108 CFU/ml. Plant roots were wounded before inoculation by cutting with the knife. Seven accessions including IT 32899 were rated as resistant, while other 98 accessions were rated as susceptible. IT 32899 scored 0.1 of disease rate and 0.7 of disease index. The selected accessions will be used as a material to reveal the mechanism of wilt tolerance and to identify the host gene involved in defense response.

인삼 개갑종자의 초저온보존 효과 및 최적조건을 구명하기 위하여 건조 조건과 수분함량 및 액체질소 보존 전 동결처리와 보존 후 해동처리에 따른 초저온보존 효과를 조사하였다. 건조처리는 25℃ air flow 챔버(RH 10～12%)와 15℃ 건조실(RH 22～25%)을 이용하여 실시하였고 보존 전 동결은 –24℃에서 30일간 처리하였으며 보존 후 해동은 4℃에서 24시간, 40℃에서 2분 처리구를 각각 비교하였다. 그 결과 15℃에서 종자수분 8-12%로 건조된 종자가 보존 전 처리 없이 액체질소에 보존된 후 40℃에서 해동되었을 때 발아율이 가장 높은 것으로 나타났다. 그러나 종자수분이 2.2% 이하일 때 건조가 지속되면 발아율은 감소하는 것으로 나타났으며, 4% 이하에 이르면 보존 전후 처리효과는 나타나지 않는 것으로 조사되었다. 반면 25℃에서 건조된 종자는 상대적으로 높은 발아율을 유지하였다. 건조 및 초저온보존에 의한 인삼 개갑 종자의 유근 및 자엽 생장율을 조사한 결과, GA배지 치상 10일 후 무처리한 대조구에서 첫 유근이 출현하였으며 3주 후에는 자엽발달이 시작되었다. 30일째에는 자엽생장율이 유근발달과 함께 유의하게 증가하는 것으로 나타났다. 치상 30일까지 초저온보존에 의한 생장율은 같은 양상을 보이면서 대조구보다 현저히 낮게 나타났으나 40일째에는 양상은 같았으나 회복율이 대조구보다 증가세를 보였다. 또한 보존 전 동결처리 없었던 종자는 동결처리한 종자보다 모든 처리구에서 높은 유근 및 자엽 생장율을 보였다.

The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, also extending to the temperate regions of South America and tropical. However, utilization of SSR markers have not been investigated extensively in Vicia species as compared to other crop species. Here, we have assessed the potential for transferability (cross-species amplification) of cDNA microsatellites markers developed from common vetch (Vicia sativa subsp. sativa). In total, 226 alleles were detected in 36 microsatellite loci. The number of alleles per marker ranged from one to 20, with an average of 6.3. The gene diversity and polymorphism information content value averaged 0.540 and 0.503, with a range of 0–0.85 and 0–0.84 respectively. For transferability of the SSRs, amplification was carried out with selected from two to 8 accessions of 22 different Vicia species. For individual species, the successful amplification rate ranged from 32.6% in V. ervilia to 81.9% in V. sativa subsp. nigra, with average of 48.8%. As the rate of successful amplification of microsatellite markers generally correlates with genetic distance, these SSR markers are potentially useful in the analysis of genetic relationships between or within Vicia species.

Rice (Oryza sativa L.) is important crop as a staple carbohydrate sources and was regarded as domesticated in Asia region. The seed dormancy is one of the domesticated traits, and this character allows that plants survive in various environment. The degree of dormancy have been targeted for controling in breeding program while weak dormancy cause pre-harvest sprouting contrary to the nonuniform seed germination by strong dormancy. In this study, we surveyed the variation of germiability in diverse rice genetic resources including japonica and indica ecotype. Overall, the degree of seed dormancy of rice germplasm was distinguished into four groups and admixed types; first group(G-1) included accessions revealing high germiability, the accessions of second group(G-2) acquired the high germiability by after-ripening process, third group(G-3)’s accessions showed high germiability after dormancy breaking process and the accessions of last group(G-4) maintained the low germiability in spite of the dormancy breaking process. Among the 51 japonica accessions G-1, G-2, G-3 and G-4 included 15, 15, 11 and 10 accessions, respectively, and among 40 indica accessions, G-1, G-2, G-3 and G-4 groups included 15, 15, 9 and 1 accessions, respectively. Based on these primary test, we plan to detail examine the seed germiability and survey the genetic factors affecting the degree of germiability in representative accessions of each group

The world-wide capacity of genebanks for ex-situ conservation of crop genetic resources has increased greatly since the 1970s, improving the access of crop breeders to landraces, and wild relatives (Wright, 1997). In south Korea, systematic seed germplasm management was begun at the Rural Development Administration (RDA) in 1985 and consequently genebank system was established in 1988. The RDA genebank, National Agrobiodiversity Center (NAC) of south Korea, preserve 173,217 accessions of plant seed germplasm and 10.7% (18,509 accessions) of whole preserved germplasm is occupied by horticultral PGR by 2011. Horticultural PGR preserved in NAC is composed with 67 genera of crops including pepper (3,817 accessions), tomato (2,040 accessions), cabbages (1,690 accessions) and etc. According to horticultural plant sub-group, fruit-vegetable is the biggest group with 60% of accession share and leafy vegetable (19%), root vegetable (10%), bulb vegetable (8%), and ornamental (3%) follow subsequently. In aspect of PGR status, landrace is dominant part of conserved accession and then developed cultivar, wild relatives, cultivated material, wild, and weedy type follow subsequently. Recently characterization and evaluation of horticultural PGR have been activated for 3 years in NAC, which are useful for breeders and users focused on disease-resistance or active ingredient. It will promote utilization of horticultural PGR and development of horticultural crop breeding program for both human being and sustainable agriculture in near future.

For providing basic data on estuary conservation and pattern of vegetation succession of sand bar, the flora of delta was investigated from March 2004 to August 2006 in the Nakdong River estuary of Korea. The flora in the eight surveyed areas was composed of total 159 taxa that belonged to 46 families, 123 genera, 133 species, 1 subspecies, 20 varieties, and 5 forms. Among them, naturalized plants and evergreen broad trees were 31 taxa and 5 taxa, respectively. The communities of main species at delta of estuary were composed of 16 species such as Rosa rugusa community, Scirpus triqueter community, Phragmites communis community, Carex kobomugi community, Digitaria ciliaris community. Especially, Rosa rugusa community which are important to establish the southern limit line of distribution as the northern factor was distributed through Jinwoodo. The succession of main vegetation community in the new delta (Doyodeung) was in order of Carex scabrifolia, Scirpus triqueter, Phragmites communis, Imperata cylindrica var. koenigii and Miscanthus sinensis var. purpurascens. Therefore, the succession order of the surveyed area seems to predict the pattern of the vegetation succession of another deltas in Nakdong River estuary.

“고강”은 고품질･내병･다수성 지황 개발을 목표로 지황1호 실생 집단에서 우량 개체를 선발, 증식을 거친 후 2001～’02년 생산력 검정시험을 실시하였다. 그 결과 고품질이면서 병해에 강하고수량성이높아수원7호로계통명을부여한후, 2003～’05년까지 3년간 지역적응시험을 실시한 결과 우수성이 인정되어 2005년 12월 직무육성 품종 심의회를 거쳐 “고강”으로 명명하였는바 그 주요특성을 요약하면 다음과 같다. 1. 고강의 초형은 지면에서 약간 솟아오르는 형태를 나타내며, 생육중기 완전 전개된 잎중 가장 어린잎들의 색깔은 안토시아닌 색소를 거의 띠지 않아 대비품종과 구별된다. 2. 고강은 지황1호에 비하여 잎이 크고 많으며, 뿌리가 굵고 길다. 3. 고강은 병에 대한 저항성도 비교적 강하여 생산의 안정성이 높은 품종이다. 4. 고강은 2003～’05년까지 3년간 실시한 지역적응시험결과 수원 등 3지역에서 모두 증수되는 것으로 나타났고, 3지역의 10a당 평균 수량은 1,186kg로 지황1호 대비 13% 증수하였다. 5. 고강은 지황의 주요성분인 catalpol과 엑스함량이 지황1호에 비하여 많은 고품질 품종이다.

Kalopanax pictus is a long-lived woody species mostly distributed in East Asia. K. pictus has been regarded as medically and ecologically important species in Korea. Thornless castor aralia variant, local name 'Cheongsong' is an endemic to Cheongsong province in Korea. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and structure of Korean populations of two species. A high level of genetic variation was found in six K. pictus populations. Twelve primers revealed 49 loci, of which 29 were polymorphic (59.2%). Nei's gene diversity for K.pictus and K. pictus variant were 0.119 and 0.098, respectively. Mean of genetic diversity in K. pictus was higher than average values for species with similar life history traits. The asexual and sexual reproduction, perennial habitat, and longevity are proposed as possible factors contributing to high genetic diversity. An indirect estimate of the number of migrants per generation (Nm=0.857) indicated that gene flow was not extensive among Korean populations of K.pictus. It is suggested that the isolation of geographical distance and reproductive isolation between K.pictus and K.pictus variant populations may have played roles in shaping the population structure of this species.