현재 양송이 품종의 개발은 1980년대에 개발된 방법에 의존하여 진행되고 있다. 유전자가위를 이용한 유전자교정 기술이 다양한 분야에서 각광받고 있고, 이 기술을 버섯 육종에도 적용하기 위하여 진행된 이 연구에서는, CRISPR/Cas9 활용에 필수적인 원형질체 분리 효율을 1.0 × 10 8 /mL까지 안정적으로 끌어올렸고, spermidine을 이용하여PEG 형질전환의 효율 또한 기존 방법에 비해 100배가량 끌어올렸음을 보고한다.

The production scale of mushroom cultivation in Korea is approximately 600 billion won, which is 1.6% of the Korean gross agricultural output. Annually, ca. 190,000 tons of mushrooms are harvested in Korea. The oyster mushroom is one of the most favorite and commonly consumed mushrooms in Korea. In case of breeding, the protoplast fusion technique of the oyster mushroom, P. ostreatus was first commercialized in the world. To develop the high temperature varieties, various examinations were accomplished. Protoplast fusion of abalone mushroom, high temperature, and oyster mushroom, popular mushroom, were attempted. 2 strains of P.ostreatus and 2 strains of P.abalonus were fused to each other by protoplast isolation. Fusion strains were investigated by random amplified polymorphic DNA(RAPD) marker, and selected strains were cultivated at 25°C after completing the mycelial growth. As a result of sawdust bag cultivation, most of strains showed the fruiting body, but the morphological characteristics among them were not significant different. However, these protoplast fusion strains were expected as new parents strains to develop varieties.