본 연구는 유색칼라(Zantedeschia)를 고령지 농업연 구센터 대관령포장(해발 800m)의 PE필름(polyethylene film) 하우스에서 단경기 생산의 가능성과 생장, 개화 반응, 절화특성을 구명하고자 수행하였다. 유색칼라인 ‘Golden Affair’ 품종으로 구경 3.5cm, 구고 1.3cm, 구중 17.0g의 구근을 이용하였다. 정식 1일전에 200mg • L-1 GA3에 30분간 침지한 다음 밤 동안 상 온에 두었다가 12~14시간 후인 다음날 아침에 햇빛에 건조시킨 후 5월 19일 차광처리별(0, 30, 50, 70%) 로 정식하였다. 초장은 70% > 50% > 30% >무차광 순으로 양호하였고, 엽수는 50%차광에서 가장 좋았으 며 엽장, 엽폭도 같은 경향이었으나 유의차는 나타나지 않았다. 시기별 초장의 변화는 90일까지 급격하게 증 가하다가 그 이후는 서서히 증가되었다. 개화기는 무 차광 > 30%> 50%> 70% 차광에서 각각 6월 29일, 7월 9일, 7월 15일, 8월 28일 개화되어 차광율이 적 을수록 빨리 개화되었다. 절화장 등 품질 면에서는 70% > 50% > 30% > 무차광 순으로 차광이 많을수록 품질이 좋은 경향이었고 절화수량 면에서는 1등급 절 화수량이 50%차광에서 가장 많게 나타났다. 차광처 리별 수확기에 따른 절화장의 변화는 수확시기가 늦 을수록 길게 나타나 결국은 생장이 충분하게 되는 후기에 절화품질이 좋은 것으로 나타났다.

Potentilla kleiniana is a perennial herb beloning to Rosaceae family. Herein we investigated the effect of light intensity, light quality and chemical elicitor on plant growth and the accumulation of bioactive compounds in P. kleiniana. After 60 days of cultivation under different shading level [0% (200 μmol·m -2 ·s -1 ), 35% (95 μmol·m -2 ·s -1 ), 55% (65 μmol·m -2 ·s -1 ), 75% (40 μmol·m -2 ·s -1 )] in the greenhouse, chlorophyll and carotenoid content were the highest under 35% treatment, however, plant height, leaf number and biomass were the highest under non-shading. As a result of cultivation among strong light condition as a control, florescence and three mixture light sources [red:white:blue (RWB) = 8:1:1, red:blue (RB) = 8:2, red:green:blue (RGB) = 8:1:1] as treatments in plant growth chamber (25 ± 2℃, 185 ± 3 μmol·m -2 ·s -1 ), growth, biomass, chlorophyll content low difference between total phenolic compouds and flavonoid content were higher under RWB treatment. DPPH radical elimination ability was the highest under all treatments especially florescence and RGB treatment except control. As a result of treating chemical elicitor [salicylic acid (SA), methyl jasmonate (MeJA)] concentration (0, 50, 100, 200 μM) respectively, plant height, petiole diameter and biomass were higher under non-treatment, MeJA 50 μM. It was investigated that fresh weight and dry weight under MeJA 50 μM treatment were especially a little high. Total phenolic compounds and flavonoid content of SA 50 μM treatment was the highest but DPPH radical elimination ability was significantly the highest under MeJA 200 μM (88.65%) and MeJA 50 μM (87.84%) treatment. Thus, this study suggested that we determined optimal shading and light quality in the greenhouse and plant growth chamber also confirmed bioactive compound content, antioxidant ratio increase according to different chemical elicitation concentration.

Echinacea purpurea is an important medicinal plant native of North America, which contains caffeic acid derivatives, alkamides, glycoproteins, and polysaccharides. For commercial roduction of bioactive compounds from Echinacea purpurea, adventitious roots were induced and proliferated in 5 and 20-liter bioreactors under various cultural conditions (Medium composition, inoculum density, air volume, light, temperature et al.). In a 5-liter bioreactor, more than 10-fold of biomass increment was achieved after 5 weeks of culture. Increases of root biomass and bioactive compounds were correlated with increased consumption of sucrose and macro/micro nutrients in the medium. Elicitations have been conducted with UV-C, NO, and SA to increase the accumulation of total polyphenolics, flavonoids and caffeic acid derivatives in the adventitious root cultures, which resulted in 1.2-1.6 times of increment. Based on the previous experimental results, 500-liter and 1000-liter scale bioreactors were applied for large scale cultures of Echinacea purpurea adventitious roots. Total root biomass of 3.62 kg and 5.05kg (dry weight) were achieved in a 500 L and a 1000 L bioreactor, respectively. The accumulation of 22.55 mg/g DW cichoric acid, 4.92 mg/g DW chlorogenic acid and 3.99 mg/g DW caftaric acids were also achieved from the adventitious root in a 1000-liter scale bioreactor.