A new variety of Alfalfa (Medicago sativa L.), named 'Alfaking' was developed between 2015 and 2023 at the Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Republic of Korea. The variety was produced through artificial hybridization, with ‘Paravivo’ serving as the maternal line and ‘WL514’ as the paternal line. ‘Alfaking’ underwent field tests across four regions (Cheonan, Pyeongchang, Jeongeup, and Jinju) to evaluate its agronomic characteristics and forage production over two years (2022-2023). The dry matter yield of ‘Alfaking’ reached 22,516 kg/ha, which is 11% higher than the control variety, ‘Vernal.’ ‘Alfaking’ exhibited 2.1% higher crude protein content than ‘Vernal’ in forage nutritive value. The development of this new alfalfa variety, which exhibits excellent adaptability to challenging environmental conditions, is expected to enhance forage cultivation and productivity in Korea.

Iron (Fe) is a vital element for plants and other organisms, involving in several physiological processes including respiration, chlorophyll biosynthesis, and photosynthesis. Unfortunately, how Fe accumulation regulates in response to light quality has not been well established in plants. Therefore, the aim of the study was to explore the mechanism of Fe homeostasis by light quality. In this study, we found morpho-physiological attributes were significantly improved in response to blue (λmax: 450) compared to white (λ max: 500) and red (λmax: 660) light. The root-shoot length, plant biomass, photosynthesis efficiency (Fv/Fm) and leafgreen (SPAD) significantly declined in response to white and red light. However, these parameters were improved and iron deficiency was substantially alleviated by blue light exposure in alfalfa seedlings. This study might be useful to the forage breeders and farmers for improving alfalfa yield and nutritional benefits.

We investigate the astronomical phenomenon records of Sukjong’s 𝐶ℎ𝑢𝑛𝑏𝑎𝑛𝑔-𝐼𝑙𝑔𝑖 made by 𝑆𝑖𝑔𝑎𝑛𝑔𝑤𝑜𝑛 (Royal Educational Office of the Crown Prince) at which King Sukjong was the crown prince (i.e., 1667 January 24–1674 September 22). From the daily records of 2,799 days, we extract the astronomical records of 1,443 days and classify them into 14 categories. Then, we group the records of each category into five phenomena (Atmosphere, Eclipse, Daylight Appearance, Apparition, and Appulse) and compare them with the results of modern astronomical computations wherever possible. Except for Atmosphere group comprising records of meteorological events, such as solar halo, lunar halo, and unusual clouds, the significant findings in every other group are as follows: In Eclipse group, the solar eclipse that occurred on 1673 August 12 was unobservable in Korea, which is in contrast to the record of 𝐽𝑜𝑠𝑒𝑜𝑛𝑤𝑎𝑛𝑔 𝑗𝑜-𝑆𝑖𝑙𝑙𝑜𝑘 (Annals of the Joseon Dynasty), which states that the sun was in eclipse around sunset time, as observed at 𝑁𝑎𝑚 mountain. From the lunar eclipse records, we verify that the Joseon court did not change the date of the events observed after midnight. In Daylight Appearance group, we confirm that this phenomenon was observed during the daytime and not during twilight. We further suggest that if observation conditions are met, a celestial body brighter than −2.3 mag could be seen during the daytime with the naked-eye. In Apparition group, we find the possibilities that the Orionid meteor shower had influence on the meteor records and the seasonality on the aurora records. We also find that the Korean records in which the coma of comet C/1668 E1 was located below the horizon were overlooked in previous studies. Finally, we find that the records of Appulse group generally agree with the results of modern calculations. The records of 𝐵𝑒𝑜𝑚 (trespass in literal) and 𝑆𝑖𝑘 (eating in literal) events show average angular separations of 1.2◦ and 1.0◦, respectively. In conclusion, we believe this work helps study the astronomical records of other logs of 𝑆𝑖𝑔𝑎𝑛𝑔𝑤𝑜𝑛, such as Sunjong’s 𝐶ℎ𝑢𝑛𝑏𝑎𝑛𝑔-𝐼𝑙𝑔𝑖.

Drought stress is a condition that occurs frequently in the field, it reduces of the agricultural yield of field crops. The aim of the study was to screen drought-adapted genotype of Italian rye grass. The experiments were conducted between the two Italian ryegrass (Lolium multiflorum L.) cultivars viz. Hwasan (H) and Kowinearly (KE). The plants were exposed to drought for 14 days. The results suggest that the morphological traits and biomass yield of KE significantly affected by drought stress-induced oxidative stress as the hydrogen peroxide (H2O2) level was induced, while these parameters were unchanged or less affected in H. Furthermore, the cultivar H showed better adaptation by maintaining several physiological parameter including photosystem-II (Fv/Fm), water use efficiency (WUE) and relative water content (RWC%) level in response to drought stress. These results indicate that the cultivar H shows improved drought tolerance by generic variation, improving photosynthetic efficiency and reducing oxidative stress damages under drought stress. These findings can be useful to the breeder and farmer for improving drought tolerance in Italian rye grass through breeding programs.

Fescues, which are widely cultivated as grasses and forages around the world, are often naturally infected with the endophyte, Epichloë. This fungus, transmitted through seeds, imparts resistance to drying and herbivorous insects in its host without causing any external damage, thereby contributing to the adaptation of the host to the environment and maintaining a symbiosis. However, some endophytes, such as E. coenophialum synthesize ergovaline or lolitrem B, which accumulate in the plant and impart anti-mammalian properties. For example, when livestock consume excessive amounts of grass containing toxic endophytes, problems associated with neuromuscular abnormalities, such as convulsions, paralysis, high fever, decreased milk production, reproductive disorders, and even death, can occur. Therefore, pre-inoculation with non-toxic endogenous fungi or management with endophyte-free grass is important in preventing damage to livestock and producing high-quality forage. To date, the diagnosis of endophytes has been mainly performed by observation under a microscope following staining, or by performing an immune blot assay using a monoclonal antibody. Recently, the polymerase chain reaction (PCR)-based molecular diagnostic method is gaining importance in the fields of agriculture, livestock, and healthcare given the method’s advantages. These include faster results, with greater accuracy and sensitivity than those obtained using conventional diagnostic methods. For the diagnosis of endophytes, the nested PCR method is the only available option developed; however, it is limited by the fact that the level of toxic alkaloid synthesis cannot be estimated. Therefore, in this study, we aimed to develop a triplex real-time PCR diagnostic method that can determine the presence or absence of endophyte infection using DNA extracted from seeds within 1 h, while simultaneously detecting easD and LtmC genes, which are related to toxic alkaloid synthesis. This new method was then also applied to real field samples.

Pollution of agricultural soil by alkaline salts, such as Na2CO3, is a critical and long-lasting problem in cultivable land. The aim of the study was to examine the putative role of citric acid (CA) in alleviating Na2CO3-stress in alfalfa. In this study, Na2CO3 significantly induced leaf chlorosis, inhibited plant growth and photosynthesis related parameters, increased hydrogen peroxide (H2O2) and reduced major antioxidant enzymes (SOD ,CAD, APX) in alfalfa. However, the presence of CA these negative effects of Na2CO3-stress largely recovered. Interestingly, expression of antioxidant and ion transporter genes (Fe-SOD, CAT, APX, DHAR and NHX1) involved in Reactive oxygen species (ROS) homeostasis and oxidative stress tolerance in alfalfa. These findings suggest that CA-mediated Na2CO3 stress alleviation is an ecofriendly approach that would be useful to local farmer for alfalfa and other forage crop cultivation in alkaline soils.

Cadmium (Cd) toxicity is a serious limitation for agricultural production. In this study, we explored tolerance mechanism associated with Cd toxicity tolerance in alfalfa plants. We used three distinct alfalfa cultivars M. sativa cv. Vernal, M. sativa cv. Zhung Mu, and M. sativa cv. Xing Jiang Daye in this study. Cd showed declined chlorophyll score in Xing Jiang Daye compared with Zhung Mu and Vernal. No significant change observed among the cultivars for root and shoot length. Atomic absorption spectroscopy analysis demonstrated a significant accumulation of Cd, Fe, S and PC in distinct alfalfa cultivars. However, Zhung Mu and Xing Jiang Daye declined Cd accumulation in root, where Fe, S and PC incremented only in Zhung Mu. It suggests that excess Cd in Zhung Mu possibly inhibited in root by the increased accumulation of Fe, S and PC. This was further confirmed by the response of Fe (MsIRT1) and S transporters (MsSULTR1;2 and MsSULTR1;3), and MsPCS1 genes associated with Fe, S and PC availability and translocation in roots and shoots. It suggests that specially the transcript signal inducing the responses to adjust Cd especially in Zhung Mu. This finding provides the essential background for further molecular breeding program for forage crops.

Soil acidity or alkalinity are serious limitations for crop production. The purpose of this study was to clarify the negative effects of extreme pH stress (low and high) on alfalfa vegetative growth (VG) and biomass accumulation (BA). Two-week-old alfalfa seedlings were exposed to different pH (4.0, 4.5, 7.0, 8.0 and 8.5, respectively) levels for 72 hours. Alfalfa grown at pH 4.0 and 8.5 significantly reduced VG and BA, wherein as neutral pH (7.0) comparably exhibited better growth and biomass yield. These results indicate that extreme acidic or alkaline level are critical limiting factors for growth and biomass yield in alfalfa.

Glutathione S-transferase (GST) is a key gene involved in multiple stress tolerance in all living organisms, though it is still to be disclosed the gene function in teff grass [Eragrostis tef (Zucc.)Trotter].The objectives of this study were to clone and molecular characterization of GST gene in teff grass. We characterized GST1 from teff grass (EtGST1), it composed of a 645-bp open reading frame (ORF) that encoded 195 amino acid residue. Further, we transformed EtGST1 in E.coli BL21 (DE3) cells. This recombinant EtGST1 in E.coli BL21(DE3) induced at 37°C temperature. In addition, Growth of cells overexpressing EtGST1 rapidly increased in the presence of polyethylene glycol (5%), heat (46°C), NaCl (0.6%), and arsenic (1 mM) than that of cells harboring an empty vector. These results suggest that EtGST1 would be suitable candidate for improving tolerance in forages and/or grasses species against multiple abiotic stresses.

We analyze the time data recorded in Korean astronomical almanacs for the years from 1913 to 1945, which belong to the period in which Japan occupied Korea (1910--1945). These almanacs, published by Japanese scholars, differ from previous almanacs in terms of organization, content, and calendrical methods. In this study, we first extract twelve kinds of time data from the almanacs at the following times: solar terms, rising and setting of the Sun and Moon, transit of the Sun, phases of the Moon (i.e., new Moon, first quarter Moon, full Moon, and last quarter Moon), and eclipses of the Sun and Moon. Then, we compare the time data with that obtained from modern calculations. Even though all time data in the almanacs are tabulated in units of minutes, we calculate the data in units of seconds and determine the root mean square (RMS) deviation values for each kind of time data to estimate the accuracy of the data. Our findings are as follows: First, the kind and tabulation method of time data changes several times. For instance, solar transit time is listed only for six years from 1937 to 1942. Second, the times of two equinoxes and those of a new Moon are considerably close to midnight. Third, there are some typographical errors in the almanacs, particularly in the times of moonrise and moonset. Fourth, the contact times for lunar eclipses represent the times of the umbra and not of the penumbra, which is different from the times for solar eclipses. Finally, the RMS deviation values are approximately 0.5 min on average in all kinds of time data, even though they show slightly large differences in the times related to the Moon. In conclusion, we believe that this study is useful for investigating the time data in the almanacs of other East Asian countries that were published during the same period, such as China, Japan, and Manchuria.

Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of H2O2 and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

In this paper, we study the lunar eclipse records in the Goryeosa (History of the Goryeo Dynasty), an official history book of the Goryeo dynasty (A.D. 918 – 1392). In the history book, a total of 228 lunar eclipse accounts are recorded, covering the period from 1009 to 1392. However, we find that two accounts are duplications and four accounts correspond to no known lunar eclipses around the dates. For the remaining lunar eclipses, we calculate the magnitude and the time of the eclipse at different phases using the DE406 ephemeris. Of the 222 lunar eclipse accounts, we find that the minimum penumbral magnitude was 0.5583. For eclipses which occurred after midnight, we find that some accounts were recorded on the day before the eclipse, like the astronomical records of the Joseonwangjosillok (Annals of the Joseon Dynasty), while others were on the day of the lunar eclipse. We also find that four accounts show a difference in the Julian dates between this study and that of Ahn et al., even though it is assumed that the Goryeo court did not change the dates in the accounts for lunar eclipses that occurred after midnight. With regard to the contents of the lunar eclipse accounts, we confirm that the accounts recorded as total eclipses are accurate, except for two accounts. However, both eclipses were very close to the total eclipse. We also confirm that all predicted lunar eclipses did occur, although one eclipse happened two days after the predicted date. In conclusion, we believe that this study is very helpful for investigating the lunar eclipse accounts of other periods in Korea, and furthermore, useful for verifying the calendar dates of the Goryeo dynasty.

The present research investigated copper and cadmium stress-induced differentially expressed genes (DEGs) using annealing control primers (ACP) with the differential display reverse transcription polymerase chain reaction technique in alfalfa (Medicago sativa L. cv. Vernal) leaves. Alfalfa leaves were subjected to 250 μM of copper and cadmium treatment for a period of 6 h. A total of 120 ACPs was used. During copper and cadmium treatment, 6 DEGs were found to be up or down regulated. During copper stress treatment, 1 DEG was up-regulated, and 3 novel genes were discovered. Similarly, during cadmium stress treatment, 1 DEG was up-regulated and 5 novel genes were identified. Among all 6 DEGs, DEG-4 was identified as the gene for trans-2,3-enoyl-CoA reductase, DEG-5 was identified as the gene for senescence-associated protein DIN1 and DEG-6 was identified for caffeic acid O-methyltransferase. All the up-regulated genes may play a role in copper and cadmium stress tolerance in alfalfa.

This study investigated the initial mass function (IMF) and star formation history of high-mass stars in the Small Magellanic Cloud (SMC) using a population synthesis technique. We used the photometric survey catalog of Lee (2013) as the observable quantities and compare them with those of synthetic populations based on Bayesian inference. For the IMF slope (γ) range of -1.1 to -3.5 with steps of 0.1, five types of star formation models were tested: 1) continuous; 2) single burst at 10 Myr; 3) single burst at 60 Myr; 4) double bursts at those epochs; and 5) a complex hybrid model. In this study, a total of 125 models were tested. Based on the model calculations, it was found that the continuous model could simulate the high-mass stars of the SMC and that its IMF slope was -1.6 which is slightly steeper than Salpeter's IMF, i.e., γ=-1.35.

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

We performed a BVR photometric survey for the entire Small Magellanic Cloud (~26 deg 2 ) with a mosaic system, Wide Field Imager (WFI), covering three seasons: September and October 2001 and November 2002. Through the usual data reduction procedures, we present ~0.73 million catalogue stars brighter than 19 magnitude in B amongst a total of ~1.3 million and compare them with published astrometry and photometry results. We found that the average differences between our and the published data are ~0.7 arcsec in astrometry and 0.065, 0.054, and 0.163 in B, V, and R, respectively, in photometry. In addition, using the 2dF spectroscopic data from Evans et al. (2004), we determined the color excesses in (B-V) and (V-R) to be 0.086±0.156, and 0.065±0.112, respectively, while for the distance modulus, we obtained 18.55±1.05.