11 종의 목이버섯 자실체로부터 얻은 50% EtOH추출물을 사용하여 지방전구세포인 3T3-L1의 지방세포 분화억제 효과를 확인한 모든 strain에서 억제효과가 존재하였다. 털목이에서는 21001 균주가 가장 우수하였고 흑목이에서는 21002 균주가 억제효과가 우수하였다. 전반적으로 털목이가 흑목이에 비해 지방세포 분화억제 효과가 우수하였다. 또한 Adipocyte RNA 발현량과 protein 발현량 측정 실험에서도 털목이 21001 균주가 지방세포 분화억제효과에 있어서 가장 우수하였다. 털목이 21001 버섯을 사용하여 동물실험을 진행한 결과 털목이 자실체 분말이나 50% EtOH 추출물을 급여한 실험에서 체중 감소효과가 나타났으며, 특히 EtOH 추출물 0.2%를 급여한 군에서 유의하게 감소함을 확인하였다(*P<0.05). 동물 희생 후 적출한 간 중량 및 부고환지방량과 혈청 내 중성지방 및 총 콜레스테롤, 혈당, ALT, AST 등도 털목이버섯 21001 EtOH 추출물 0.1% 이상 급여 시 고지방식이만을 급여한 군에 비해 유의차(*P<0.05 이상) 있게 감소함을 확인하였다.

The purpose of this study is to find out a food material having anti-obesity effect and suggest the effect of the material to people who are interested in anti-obesity. For this study, Auricularia (wood ear) has been used. It is world widely spread mushroom, especially in east north Asia countries. It is also called as wood ear in western countries because of its shape. The most considerable fact is that Auricularia is being used for popular food materials for cooking because of its good taste. Totally 11 strains of Auricularia were offered by Doctor Kim, Kil-ja in Jeollanam do Agricultural Research & Extension Services. There were 8 strains of Auricula-polytricha, and 3 strains of Auricula-judae. Each Auricularia strains were extracted with 50% Ethanol for 12 hours in 40°C and then 11 strains were prepared for in vitro study. In vitro study focused on the inhibition effect on adipocyte differentiation of Auricularia spp. 3T3-L1 (preadipocyte cell) was used for identifying the inhibition effect on adipocyte differentiation. Before that, MTT assay was used for measurement of cell viability. The result is essential for fixing the treat concentration of the extracts. After adipocyte differentiation, cells were stained with Oil Red O solution to dye differentiated adipocyte cells. Spectrophotometer was used to quantify the adipocyte cells. There are few adipocyte differentiation markers. From the markers, this study selected four markers (PPAR γ, FABP4(ap2), CD36, C/EBPα) and used them to measure mRNA expression using real-time PCR and Western blot experiment. As a result, this study found out that all the extract of strains has inhibition effects on adipocyte differentiation, especially the adipocyte differentiation control probability of Auricula-polytricha strain 21001 was 24.6%. Auricula-judae strain 21002 (19.3%), Auricula-polytricha strain 249 (14.0%) followed. The result concerning the inhibition effect was identified through Western blot experiment as well. As a further study, in vivo study is on progressing. 56 ICR mice (6w, male) are being used for the animal study. It consists of 8 groups. One is control, another is positive control, the other groups are Auricula-polytricha strain 21001 treatment group. Different concentration of powder or extract of Auricularia is treated in each treatment group. We are anticipating that the result of this further study will be correspond to that of in vitro study.

"Yeu", an Asiatic lily variety was released by the National Horticultural Research Institute (NHRI), in 2006. A crossing was made between Asiatic lily "Foxtrot", a orange colored cultivar, and Asiatic lily "A94-l" (White bird x Foxtrot), a orange colored cultivar in 1996. A promising line No. 9857 was selected in natural cultivation condition from the progenies of a cross combination between "Foxtrot" and "A94-1" in 1998. The flower characteristics and cultivation performance of "Yeu" were investigated from 2000 to 2005 named as Wongyo C1-85. The natural flowering time of "Yeu" was the middle of June, but year-round cut flower production was possible by storing the bulbs under -1 to -2℃ conditions. The flower has unspotted, upward facing, and orange petals without fragrance. The plant height at the natural flowering time is 50±5.7 cm. The width of flower is 12.6±0.5 cm. The weight and circumference of bulb are 18.4±6.8 g and 12.4±2.2 cm, respectively. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially in wet season.

"Black Eye", an Asiatic lily cultivar was released by the National Horticultural Research Institute (NHRI) in 2006. A crossing was made between an Asiatic lily "La Toya", a purple colored variety, and an Asiatic lily "Nerone", a dark red colored variety in 1997. Selection was made in natural cultivation condition from the progenies of a cross combination between "La Toya" and "Nerone" in 2000. The flower characteristics and performance of "Black Eye" were investigated from 2001 to 2005 as Wongyo C1-89. The natural flowering time of "Black Eye" at rain shelter condition was the middle of June, but year-round cut flower production was possible by storing the bulbs at -1 to -2℃ condition. It has black spotted and upward facing dark red petals without fragrance. The plant height is 118 cm. The width of flower is 16.8 cm. The weight and circumference of bulb are 25.9 g and 13.7 cm, respectively. For forcing culture at winter season, it is necessary to add Ca++ to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially during wet season.