11 종의 목이버섯 자실체로부터 얻은 50% EtOH추출물을 사용하여 지방전구세포인 3T3-L1의 지방세포 분화억제 효과를 확인한 모든 strain에서 억제효과가 존재하였다. 털목이에서는 21001 균주가 가장 우수하였고 흑목이에서는 21002 균주가 억제효과가 우수하였다. 전반적으로 털목이가 흑목이에 비해 지방세포 분화억제 효과가 우수하였다. 또한 Adipocyte RNA 발현량과 protein 발현량 측정 실험에서도 털목이 21001 균주가 지방세포 분화억제효과에 있어서 가장 우수하였다. 털목이 21001 버섯을 사용하여 동물실험을 진행한 결과 털목이 자실체 분말이나 50% EtOH 추출물을 급여한 실험에서 체중 감소효과가 나타났으며, 특히 EtOH 추출물 0.2%를 급여한 군에서 유의하게 감소함을 확인하였다(*P<0.05). 동물 희생 후 적출한 간 중량 및 부고환지방량과 혈청 내 중성지방 및 총 콜레스테롤, 혈당, ALT, AST 등도 털목이버섯 21001 EtOH 추출물 0.1% 이상 급여 시 고지방식이만을 급여한 군에 비해 유의차(*P<0.05 이상) 있게 감소함을 확인하였다.

Hot water extracts from 16 domestic edible mushrooms including Pleurotus ferulae (Lanzi) X.L. Mao, Lentinula edodes (Berk.) Pegler, and Hypsizygus marmoreus (Peck) H.E. Bigelow, which are commercially available, were used for determining the cosmetic potential of these mushrooms. In this study, we carried out in vitro functional experiments to determine the inhibitory effects of these extracts on L-DOPA oxidation of tyrosinase and melanin synthesis. Based on the results of these experiments, H. marmoreus (Peck) H.E. Bigelow No. 10 and No. 15 were selected for further analysis. We analyzed the melanin synthesis inhibitory activity, TRP1 and MITF expression via real-time PCR, and Fontana Masson staining in artificial skin Neoderm®-ME. Taken together, we observed that the hot water extract from H. marmoreus (Peck) H.E. Bigelow (No. 15) had better whitening effect than the extracts of other mushrooms. Thus, it can be a potential source of skin-whitening agent

국내 시판 식용버섯 중 아위버섯 및 황금송이버섯, 표고 등을 포함한 16종을 열수추출하여 추출물의 미백 및 주름개선 등의 향장학적 기능성을 확인하였다. 피부 keratinocytes인 HaCaT cell에 각각의 열수추출물을 1,000 μg/ml 및 100μg/ml, 10μg/ml 농도로 하여 실험한 결과 모든 농도에서 cytotoxic은 발견되지 않았으며, wound healing assay를 통하여 cell migration과 proliferation을 확인할 수 있었다. 미백효과를 확인하고자 tyrosinase inhibition 및 DOPA oxidation inhibition, Melanin contents를 분석한 결과 갈색백만송이버섯 (Hypsizygus marmoreus (Peck) H.E. bigelow)과 만가닥버섯(Hypsizygus marmoreus (Peck) H.E. bigelow)이 다른 버섯의 열수 추출물에 비하여 가장 우수한 효능을 나타냈으며 이 결과를 토대로 2 종의 버섯 열수추출물을 이용하여 인공피부 Neoderm®-ME로 기능성을 확인하였다. 또한, 주름개선 및 광노화 억제에 대한 효과를 확 인하고자 elastase inhibitory activity, collagenase inhibitory activity 및 UVB 조사 후 MTT assay를 분석한 결과 흰색백만송이버섯(Hypsizygus marmoreus (Peck) H.E. bigelow)과 갈색백만송이버섯 (Hypsizygus marmoreus (Peck) H.E. bigelow)이 다른 버섯 추출물에 비하여 가장 우수한 효능을 나타냈으며 이결과를 토대로 인공피 부 Neoderm®-ED로 그 기능성을 확인하였다.

This paper intends to analyze the behavior characteristics of steel storage racks according to the constraints conditions by external loads such as seismic loads. In order to achieve these goals, the steel storage racks generally used in Korea were selected as target structures and shaking table tests were conducted. In order to confirm the behavior according to the constraints conditions, the foundation of the steel storage racks was constructed with three conditions (1 bolt, 4 bolts, fixed) and the experiment was carried out. As a result of the analysis of the data obtained through the experiment, it was confirmed that additional damper installation is needed rather than changing the condition of the bottom plate in order to secure the safety of the steel storage racks.

As the number of clinical studies in Korea is increasing and qualitatively improving, interest in safety and welfare of research subjects, which are subjects of clinical research, is also increasing. Researchers who conduct research on humans should know and follow established laws and regulations in relation to clinical research, be aware of research ethics guidelines, and voluntarily comply with them. We would like to briefly inform the researchers that the IRB (Institutional Review Board) of Chosun University Hospital, which was enacted in June 2016 and revised in September 2018, will be able to fully understand the ‘Ethics Guideline for Research Related Persons in Chosun University Hospital (Version 2.0)’. Therefore, researchers need to understand and comply with domestic laws and regulations regarding clinical research, understand the roles and responsibilities of IRBs in protecting research subjects, and study research ethics guidelines.