본 연구는 잎새버섯 병 재배시 적합한 배지조성비율을 구명하고자 수행되었다. 배지조성은 참나무톱밥: 옥수수피: 건비지를 건물중량 비 율로 T1처리(67:11:22), T2처리(68:15:17), T3처리(74:14:12)의 3처리를 두고 시험한 결과, 발이율은 T1처리에서 72.6%, T2처리에서 72.1%로 두 처리 간 유의적 차이가 없었으나 T3처리에서는 65.8%로서 다른 두 처리에 비하여 낮았다. 이병률은 T2처리에서 4.1%, T3처리에서 3.7%인데 비하 여 T1처리에서는 9.8%로 가장 높았다. 수확률은 T1처리 에서 64.1%, T3처리에서 63.6%인데 비하여 T2처리에서 는 70.5%로 가장 높았다. 병당 자실체 중량은 T1처리에 서 85.5 g, T2처리에서 83.3 g으로 두 처리 간 유의적 차 이가 없었으나 T3처리에서는 72.4 g으로 다른 두 처리에 비하여 낮았다. 입병 수 10,000병 기준으로 재배 시 수량은 T2처리에서 587 kg으로 T1처리 및 T3처리에 비하여 각각 7% 및 28%가 높았다. 따라서 잎새버섯 병재배 시 적합한 배지조 성비율은 건조중량비율로 참나무톱밥: 옥수수피: 건비지 가 68:15:17인 것으로 나타났다.

In this study, 43 Collected strains of Grifola frondosa were tested in bottle cultivation. In rate of primordia formation, 10 strains were below 50%, 23 strains were 51~79% and 10 strains were over 80%. In pileus color, almost strains were grayish brown and only one strains were light grayish brown. Ivory and white pileus color were showed 2 strains, respectively. The fresh weight of fruit body per bottle of 25 strains were bellow 69g, that of 16 strains were 70~89g and that of 2 strains were over 90g. We selected 9 strains for parent strains of breeding with useful and various genetic characteristics.

'Gonji No.10', a new variety of Neolentinus lepideus, was bred by mating between monokaryotic strains isolated from 'GMNL66037' and 'GMNL66014' in Mushroom Research Institute, Gyonggi Province ARES in 2012. The optimum temperature for the mycelial growth was 29℃ on PDA medium and that for the primordia formation and the growth of fruiting body was 20±1℃. In the bottle cultivation of ‘Gonji No.10’, the period of spawn run was around 35 days at 22±1℃ and the period from scratching of inoculum to harvest was 27 days. In the characteristics of pilus and stipe, the thickness and diameter of pilus were 6.3mm, 52.0mm respectively. The length and diameter of stipe were 110.5mm, 21.6mm respectively and the number of available stipe was 3.4 per bottle. During the cultivation of ‘Gonji No.10’, the occurrence rate of Trichoderma spp. was 4.9%. In bottle and bag cultivation, the yields of fruiting bodies were 69.6g /900㎖ bottle, 114.2g/1kg bag respectively. When considered in the period available for sale, the shelf life was 34 days.

This study was carried out to determine the optimum dietary supplementation level of oyster mushroom in cherry salmon (Oncorhynchus masou masou). Juvenile cherry salmon averaging 5.0±0.5g (mean ± SD) were fed one of the five experimental diets containing 0, 3.5, 7.0, 10.5 and 14.0% oyster mushroom (D0, D3.5, D7.0, D10.5 and D14.0) for 12weeks. Increasing of dietary beta-glucan content were observed at a high dietary oyster mushroom powder. After the feeding trial, average weight gain (WG) of fish fed D0, D3.5 and D7.0 diets were significantly higher than those of fish fed D10.5 and D14.0 diets (P < 0.05), however there were no significant differences in WG among fish fed D0, D3.5 and D7.0 diets (P > 0.05). Average feed efficiency (FE) of fish fed D0, D3.5 and D7.0 diets were significantly higher than those of fish fed D10.5 and D14.0 diets (P < 0.05), however there were no significant differences in FE among fish fed D0, D3.5 and D7.0 diets (P > 0.05). Average hepatosomatic index (HSI) of fish fed D0, D3.5, D7.0 and D10.5 diets were significantly higher than those of fish fed D14.0 diets (P < 0.05), however there were no significant differences in HSI among fish fed D0, D3.5, D7.0 and D10.5 diets (P > 0.05). Therefore, these results indicated that the optimum dietary supplementation level could be greater than 3.5%, but less than 7.0% in juvenile cherry salmon under our experimental conditions. And additional research on the immune response will be necessary to carry out.

춘추느타리 2호의 최적 환기횟수는 발이유도기 1/10h-1, 생육초기 1/6h-1, 생육중기와 생육후기 1/4h-1에서 자실체의 생육이 양호하였고, 수한느타리 2호는 발이유도기 1/6h-1, 생육초기 1/6h-1, 생육중기 1/4h-1, 생육후기 1/20h-1에서 자실체의 생육이 양호하였다. 춘추느타리 2호 및 수한느타리 2호 원기형성기에는 이산화탄소의 농도의 영향이 크게 나타나지 않았지만, 자실체 생육시 1,500ppm 이상일 경우 갓의 끝부분이 위로 말려 올라가는 등 환기장해 현상이 나타났다. 이와 같은 결과로 춘추느타리 2호에 비해 수한느타리 2호의 경우 호흡량이 높아져서 환기요구도가 높았으며, 환기 횟수가 클 경우 생육이 양호하였다.