Cytochrome P450 1A2 (CYP1A2) is a member of the cytochrome P450 superfamily enzymes in mammals and plays a major role in metabolizing endogenous hormones in the liver. In recent days, CYP1A2 expression has been found in not only the liver but also other tissues including the pancreas and lung. However, little information is available regarding the expression of CYP1A2 in the ovary, in spite of the facts that the ovarian follicle growth and atresia are tightly associated with controls of endocrine hormonal networks. Therefore, the expression of CYP1A2 in the ovaries of prepubertal and pubertal rats was investigated to assess its expression pattern and puberty-related alteration. It was demonstrated that the expression level of CYP1A2 was significantly (p < 0.01) higher in the pubertal ovaries than prepubertal counterparts. At the ovarian follicle level in both groups, whereas CYP1A2 expression was less detectable in the primordial, primary and secondary follicles, the strongly positive expression of CYP1A2 was localized in the granulosa cell layers in the antral and pre-ovulatory follicles. However, the ratio of CYP1A2-positive ovarian follicle was significantly (p < 0.01) higher in the ovary of pubertal group (73.1 ± 3.1%) than prepubertal one (41.0 ± 10.5%). During the Immunofluorescence, expression of CYP1A2 was mainly localized in Fas-positive follicles, indicating the atretic follicles. In conclusion, these results suggested that CYP1A2 expression was mainly localized at the atretic follicular cells and affected by the onset of puberty. Further study is still necessary but we hypothesize that CYP1A2 expresses in the atretic follicles to metabolize residue of the reproductive hormones. These findings may have important implications for the fields of reproductive biology of animals.

Buckwheat (Fagopyrum species) has long been an excellent functional food. Besides, buckwheat sprouts contain various functional substances. In this study, we investigated the antioxidant activity of buckwheat sprouts in the context of cultivars harvested after different cultivation periods (0, 3, 5, 7, 9, 13, and 15 days after planting). Buckwheat sprouts were cultivated at 25oC for up to 15 days and then extracted with ethanol. Antioxidant components were then extracted from sprouts and leaves using a freeze dryer. The total polyphenolic content, flavonoid content, and antioxidant activity were then analyzed. The total polyphenol content increased from 32.26 mg GA eq/100 g for raw buckwheat to 114.75 mg GA eq/100 g after 7 days of cultivation. Also, the flavonoid content increased from 20.61 mg catechin eq/100 g (0 days) to 56.54 mg/g after 9 days of cultivation. The DPPH radical scavenging activity (concentration of extract at 0.25 mg/mL) increased from 7.89% at day 0 to 53.48% after 9 days of cultivation. Additionally, the ABTS radical scavenging activity increased from 10.26% at day 0 to 32.89% after 7 days of cultivation; of note, the activity decreased afterward. These results suggest that the best buckwheat sprouts with higher biological activities are those cultivated for 7-9 days. For a complete understanding of the potential of buckwheat sprouts as functional foods, we plan to further analyze their antioxidant activity in the future.

목적 : 노안을 대상으로 멀티포컬 콘택트렌즈의 디자인에 따라 처방법을 다르게 하고 각각의 처방에 따른 시기 능을 비교하였다. 방법 : CNMF(center near multifocal) 콘택트렌즈를 성공적으로 착용하고 있는 초기노안 19명(남 3명, 여 16명, 평균연령 45.47±5.88세)을 대상으로 CDMF(center distance multifocal) 콘택트렌즈와 MMV(modified monovision) 콘택트렌즈를 각각 착용하도록 하고 처방법에 따라 시기능을 비교하였다. 시력검사는 ETDRS 시표 를 사용하여 측정하였고, 근거리 입체시력은 Stereo Fly Test를 이용하였으며, 시력 만족도는 NEI-VFQ-25 설 문지를 변형하여 측정하였다. 결과 : 처방법들에 따른 원거리 교정시력, 근거리 교정시력 및 입체시력은 차이가 없었다. 그러나 원거리 시력 만족도에 대한 설문조사에서는 야외광고를 볼 때와 낮에 운전을 할 때에는 CNMF, CDMF 착용 시 MMV보다 높 았고, 밤 운전 시에는 CDMF 착용 시에 가장 높았다(p<0.050). 중간거리 시력 만족도는 컴퓨터를 사용할 때와 밤에 계단을 오르내릴 때에는 CDMF와 MMV이 CNMF보다 높았다(p<0.050). 근거리 시력만족도는 신문 및 영수증을 볼 때에는 MMV이 CNMF, CDMF보다 높았으며, 전반적인 근거리 시력 만족도는 MMV 착용 시 높았다(p<0.050). 결론 : 세 가지 노안 교정법에 따라 시력은 차이가 없었지만 일상생활에서 시력 만족도는 주시거리와 환경에 따라 차이가 발생한다. 그러므로 착용자의 요구조건에 맞는 처방법을 선택한다면 멀티포컬 콘택트렌즈 착용 만족 도를 높일 수 있을 것으로 생각된다.

Globally, colon cancer is increased gradually and known as one of the major causes of cancer death. Stevia, a substitute of sugar, is known to have many components including alpha-tocopherol and anthocyanin etc, as antioxidants. This study's purpose is to investigate whether stevia plant extract can have a protective effect against colon carcinogenesis induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in mice. Total 30 male ICR mice were divided into 2 groups; AOM/DSS treatment (control group), AOM/DSS + stevia extract (0.5%, in drinking water). After acclimation for 1 week, five weeks old mice received three intraperitoneal AOM (10 mg/kg b.w.) injections weekly for 3 weeks (0–2nd weeks of the experiment) and 2% DSS as drinking water for the next one week. AIN-76A purified rodent diet and 0.5% stevia extract water were supplied to the animals for 6 weeks. The colons of mice were collected and the number of aberrant crypt foci (ACF) and aberrant crypts (ACs) in colonic mucosa were counted after staining with methylene blue. Malondialdehyde (MDA) concentration in feces were determined. The numbers of ACF and ACs were significantly (p<0.01) decreased in stevia-treated group compared with the control group. The MDA concentration in feces was also significantly (p<0.01) decreased in stevia-treated group compared with the control group. In histopathology of colonic epithelium, hyperplasia of colonic epithelium was less observed in steviatreated group. These results indicate that stevia has a protective effect against colon carcinogenesis induced by AOM/DSS in mice and further study needs to illustrate the protective mechanisms.

A lot of work has been done on the topic of Korean traditional costume. In spite of the amount of the work, however, not much work has appeared that summarizes the overall research trend in such a way that we gain insights into the future direction of the Korean traditional costume field. This study aims to work out an analysis of the research trend that may shed light on the current status of the field and also on the things that have to be done to further develop the Korean costume design field. The analysis reported in this work is based on the articles that appeared in two journals: Journal of the Korean Society of Costume published by the Korean Society of Costume (2,661 articles from the year 1977 to 2019) and Journal of Korean Traditional Costume published by the Society of Korean Traditional Costume (762 articles from the year 1998 to 2019). The result of the analysis shows that only a very small amount of work is devoted to actual designing of Korean traditional costume, and this has to change to globalize Korean traditional fashion and draw worldwide attention to it. So more work is needed particularly on actual designing of Korean traditional costume. This does not mean that we simply have to maintain tradition and replicate the original designs, but we have to endeavor to work out novel designs by, for instance, creative destruction and restructuring, maintaining the essence of the tradition of Korean costume.

There is a growing global interest in food choices that could affect health. Consumer food choices are dictated by numerous factors such as attitudes, beliefs, desires and preferences stemming from psychological and physiological attributes. The present study aimed to understand consumers’ internal perceptions about cream soup, cream sauce, and tomato sauce using ZMET (Zaltman’s Metaphor Elicitation Technique). The 9 step ZMET interview process was conducted for a sample of 36 consumers (12 consumers for each sauce). A content analysis of the survey results was carried out. From this, 56 concepts of cream soup were derived, and 15 constructs that met with the agreement of more than one-third of the total sample consumers were extracted. These 15 constructs included ‘Soft’, ‘Recollection’, ‘Familiar’, ‘Warm’, ‘Comfortable’, etc. Similarly, for cream sauce, 67 concepts and 20 constructs ‘Silky’, ‘Warm’, ‘Restaurant’, ‘Family’, ‘Memory’, etc. were deduced. A total of 66 concepts and 20 constructs for tomato sauce ‘Sourness’, ‘Sauce Bottle’, ‘Pleasant’, ‘Ingredient’, ‘Cooking’, etc. were derived. The analysis of consumers’ consensus maps through this study provides a deep and useful understanding of consumers and their latent needs. Also, the results of this study indicate that exploring consumers’ internal perceptions is critical to understanding their healthy food choices. This can be used as basic data for formulating marketing strategies.

This study used adult wistar-based rats to observe the sexual cycle as a morphological characteristic of vaginal epithelial cells by vaginal smearing, and investigated the fetal number through mating with male rats of the same strain. The target animal was a 12 to 13-week-old Wistar-based mature unlighted rat (weight 220 g to 240 g), room temperature 23 ± 2℃, 14 hours artificial lighting (05:00 to 19:00 hours), 10 hours Adapted individuals were used for rearing for at least 2 weeks under the conditions of the darkroom (19:00 to 05:00). The feed was managed for free feeding of pellet feed for animals and water. The vaginal smearing method was used for the experiments by observing the sexual cycle every morning and confirming that the normal sexual cycle of 4 or 5 days was repeated at least 2 cycles or more. As a result, the proestrus was found to have few red blood cells, the cells and nuclei were rather large and round, and many nucleated cells were identified. In the case of the estrus, the cells were large and the nuclei were not stained, and most of the keratinocytes were found. In addition, in the metestrus and diestrus, there were many white blood cells, and it was confirmed that nucleated epithelial cells and keratinocytes were significantly reduced. The pregnancy period was 21 ± 1.8 days, and the number of live births per delivery was 11.9 on average. The number of fetuses on the 8th and 10th days of pregnancy were 15.2 ± 0.4 and 15.4 ± 0.3, respectively. On the contrary, the number of fetuses on the 12th day of pregnancy was 12.9 ± 0.6, which was significantly (p < 0.05) decreased compared to the 10th day of pregnancy, and the number of fetuses was similar until delivery. As a result of investigating the change of body weight according to the birth weight and growth stage after delivery, the birth weight of female and male was 9.2 ± 2.0 g and 9.8 ± 2.5 g, respectively. After that, until the 16th day, the female and the male showed similarly moderate weight gain, and then showed a rapid weight gain until the 21st day of lactation. With reference to the results of this study, it is expected to be used as basic data for determining the mating time of rodents and controlling pregnancy and fetal number.

The objective of the present study was to determine the best model to describe and quantify the changes in live body weight, height at withers, height at rump, body length and chest girth of Holstein cows raised under Korean feeding conditions for 50 months. The five standard growth models namely polynomial linear regression models, regression of growth variables on the first and second-order of ages in days (model 1) and regression of growth variables on age covariates from first to the third-order (model 2) as well as non-linear models were fitted and evaluated for representing growth pattern of Holstein cows raised in Korean feeding circumstances. Nonlinear models fitted were three exponential growth curve models; Brody, Gompertz, and von Bertalanffy functional models. For this purpose, a total of 22 Holstein cows raised in Korea used in the period from April 2016 to May 2020. Each model fitted to monthly growth curve records of dairy cows by using PROC NLIN procedure in SAS program. On the basis of the results, nonlinear models showed the lower root mean square of error (RMSE) for live body weight, height at withers, height at rump, body length and chest girth (12.22, 1.95, 1.55, 4.04, 2.06) with higher correlation coefficiency (R2) values for live body weight, height at withers, height at rump, body length and chest girth (0.99, 0.99, 0.99, 1.00, 1.00). Overall, the evaluation of the different growth models indicated that the Gompertz model used in the study seemed to be the most appropriate one for standard growth of Holstein cows raised under Korean feeding system.

Consumers exposed to aesthetic brands can not only experience sensory pleasure but also lead to strong customer loyalty. Furthermore, aesthetic brands can expect to lead to the overall experience of the brand. They include the brand's sensory, emotional, behavioral and intellectual experience. Since aesthetic brands related to the sensory representation of customers, consumers exposed to aesthetic brands can expect to have a meaningful impact on the overall experience of the brand. The study will select aesthetic brands that distributed in Korea and then compare the aesthetic characteristics of each brand. It also seeks to verify the brand attractiveness, consistency, prominence and influence of brand motifs on the brand's sensual, emotional, behavioral and intellectual experience. To verify the influence between variables proposed in this study, we would like to conduct a multiple regression analysis. SAS 9.4 used to verify the hypotheses proposed in this study. Cronbach's ɑ measure to verify reliability of each measurement item. In particular, brand attractiveness and brand motif can verify empirically to confirm that they play an important role in inducing brand experience.

ETc 손실을 보상하는데 필요한 물의 양을 작물 용수 요구량(Crop water requirement, CWR)로 정의되며, ETc 평가는 작물 필요 요구량을 정확하게 정량화하는 데 필요하며, 물 균형 계산에서 중요한 역할을 한다. 토마토와 파프 리카의 실제 관수 요구량(Actual crop water, ACW)이 적절한 CWR인지 평가하였다. 토마토와 파프리카 재배에 적정한 AWC 예측 및 추정을 위하여 온실 내부 환경데이터를 Penman-Monteith을 이용하여 기준 작물 증발산(ET)을 계산한 후, 기준 증발산은 작물 상수(Kc;토마토-1.15, 파프리카-1.05)계수로 조정하였다. 토마토와 파프리카의 CWR과 ACW를 계산하여 비교 평가한 결과 ACW가 CWR을 대체할 수 있지만 파프리카의 ACW는 필요 이상으로 높게 나타났다. 또한, 토마토의 ACW는 1일 100 ~ 1,200 ml이고, 파프리카의 ACW는 1일 100 ~ 500 ml가 적절한 것으로 나타났다. 그러나, 스마트 온실에서 ETc의 정밀도를 높이려면, ETc가 CWR로 변환되고 ACW와 비교하기 위해서 클래스 A팬 설정이 필요하다. 향후 실시간으로 CWR을 측정하기 위한 시뮬레이션 프로그램 연구가 필요하다.