Due to the existence of uncertainties and the unknown time variant environmental disturbances for ship course nonlinear control system, the ship course adaptive neural network robust course-keeping controller is designed by combining the backstepping technique. The neural networks (NNs) are employed for the compensating of the nonlinear term of the nonlinear ship course-keeping control system. The designed adaptive laws are designed to estimate the weights of NNs and the bounds of unknown environmental disturbances. The first order commander are introduced to solve the problem of repeating differential operations in the traditional backstepping design method, which let the designed controller easier to implement in navigation practice and structure simplicity. Theoretically, it indicates that the proposed controller can track the setting course in arbitrary expected accuracy, while keeping all control signals in the ship course control closed-loop system are uniformly ultimately bounded. Finally, the training ship of Dalian Maritime University is taken for example; simulation results illustrated the effectiveness and the robustness of the proposed controller.

The diffe rentiation of osteoblasts from mesenchymal precursors requires a series of cell fa te decis ions controlled by a hi erarchy of transcription factors Among these are RUNX2, osterix (OSX) , ATF4 and a la rge number of nuclear co-regulators. During bone development, initial RUNX2 expression coincides with the formation of mesenchyma l con densations well before the branching of chondrogenic and osteogenic lineages. Runx2 is s ubject to a number 0 1' post - tran scriptional controls including regulation by nuclear accessory factors such as ATF4 and DLX5 and post-trans lational mod ificati on, especially phosphOl‘ylation. We previously showed that Runx2-dependent transcription is acti vated by the ex tracellular signal-regulated/MAP kinase pathway in response to ECM/integrin and FGF2 stimulation. To identify and a5sess the function of ERK/MAPK phosphorylation s ites in RUNX2 and esta bli sh the role of MAPK s ignaling in bone fo 1'mation Approaches: A deletion/mutagenesis approach wa5 used to ide ntify 1'egions 0 1' RUNX2 necessary fo1' MAPK responsiveness and phosphorylation. To evaluate the in vivo function of the ERK/MAPK pa thway‘ transgen ic mice were developed wi th osteoblast- specific expression of either dominant-negative 0 1' cons titutively-active MEKl in osteoblasts and crossed with Runx2 heterozygous-• null ammals RUNX2 is phosphorylated on two critical serine res idues in the P/S/T domain. RUNX2 conta ining S/A mutations in these sites is refractory to MAPK stimula ti on while S/E muta tions cause cons titutive activation MAPK activation of RUNX2 was also found to occur in vivo 까'a n sge ni c expression of cons titutively ac tive MEKl in osteo • blas ts accelerated skeletal development while a dominant-negati ve MEKl reta rd ed bone formation in a RUNX2- dependent manner As shown by these studies‘ the ERK/MAPK pathway controls Runx2 tra nscript iona l activ ity by phosphorylation at specific serine residues. This may be a major pathway for controlling osteoblast activity in response to extracellul ar matrix signals. mechanical loads and hormonal stimulation

Bong Joon-ho’s films have a unique narrative style and profound philosophy, and have repeatedly broken box office records with high commercial value, and are deeply recognized by the world film industry and loved by audiences. This article analyzes and summarizes Bong Joon-ho’s films, discusses the creative style and thematic connotation of Bong Joon-ho films, experiences the aesthetic style of “genre narrative strategy” and “problem awareness” of Bong Joon-ho films, and thinks about the trade-off techniques behind Bong Joon-ho’s high box office and high reputation, as well as the film development model.

The essence of "ideological and political education-oriented course" is not only an educational method, but also an educational idea. The implementation of "ideological and political education-oriented course" is of great significance for implementing national ideological education and improving the effectiveness of ideological and political work in universities. It is also a necessary way to effectively play a major role in educating students in classroom. Digital Media Art courses have the unique advantages of enhancing the enjoyment and attractiveness, effectiveness and persuasion, as well as interaction and affinity in the implementation of the "ideological and political education-oriented courses" reform. It is beneficial to realize the teaching design and goal of multi-dimensional education with "ideological and political education-oriented course". In the specific implementation path, it should keep up with the times and take multiple measures. It can be pushed forward by following the principle of curriculum construction, returning to the original idea of education, applying information technology, inheriting outstanding cultural genes, and playing party committee's key role.