간행물
대한구강악안면병리학회지 KCI 등재 The Korean Journal of Oral and Maxillofacial Pathology
- 발행기관 대한구강악안면병리학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-1577 (Print)2384-0900 (Online)
- 수록기간 1977 ~ 2025
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
제31권 제5호 (2007년 10월) 38건
1.
2007.10
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to evaluate the role 0 1' integrin a 3 and integrin ß 1 in the oral squamous cell ca rcinomas. For this study‘ 10 specimens diagnosed as squamous cell carcinoma referred to the Dept. of Oral Pathology. School of Dentis try, Kyung Hee Univers ity, and 5 specimens of normal oral mucosa without any inflammatory cha nges were used as experimenta l and co nt rol groups, respectively. AlI s pecimens; experirnental and control group were f ixed in neutral f ormalin so lu tion and embedded in paraffin, and then the serial tissue section were rnade 5i1m in thickness and processed for imrnunohi stochemical observatlon The specimens were incubated with prirnary antibody against integrin a 3 r integrin ß 1‘ each was diluted at 1;100, followed by the super sensit ive non- biotin horse r adish peroxidase detection sys tem with DAB as chromogen‘ After counters ta ining with Gill ’s hematoxylin stain method and mounted and examined under the light microscope. Based on the intens ity of the immunoreactivity, intensity of the immunity was scored no ep ithelial stain, weak 0 1' focal epitheli al sta in, modera te 0 1' focal intensive epithelial stain, intense genera lized epitheli al s taining for the e pithelia l, and co nnective ti ssue component in squamous cell carcinomas, and normal oral mucosa on each Expression of integrin a 3 in t he oral mucosa was negli gible. Expression 0 1' integrin a 3 in expression in the or al s mnus cell ca rcinoma was ve ry wea k, but the express ion was increased in poorly differ entiat ed type of the oral squamous cell carcinomas ln the oral mucosa , expression of in tegr in ß 1 ra nged from weak to moderate in the cytoplasm and the cell membra nes of the kera tini zed and basal cell layer. Nuclei were mainly integrin ß 1 negative‘ but rarely revealed weak expression. ln sq uamous cell carcinoma, expression of integrin ß 1 was ntense notably in the cytoplasm, cell membrane a nd nuclear membra ne Nuclei of several tumor cells revealed moderate expression of integrin ß 1. Expression of integrin ß 1 was increased the poorly diffe rentiated type of in squamous cell carcinoma compare to that in moderate or well diffe rentiated type of oral squamous cell carCllìoma These results suggest integrin a 3 and integrin ß 1 may be influ enced the development and growth of the squamous cell carcima .
4,600원
2.
2007.10
구독 인증기관 무료, 개인회원 유료
The purpose of this study was to evaJ uate the role of integrin a 3 and integrin ß 1 expression in the saivary gJand tumors. For this study, 11 specimens diagnosed as pleomorpic adenoma, adenoid cystic carcinoma, adenocarcinoma, mucoe pidermoid carcimoma referred to the Dept. of Oral Pathology‘ School of Dentistry, Kyung Hee University, 2 specimens 01' normaJ submandibular gland tissues were used as experimental, control groups respectively, All the tissues experimental and control group wel'e fixed in neutral formaJin solution and embedded in paraffin, seriaJ tissue section were made 511m in thickness and processed in the standard way for immunohistochemical method, using primary antibody against integrin a 3, and integrin ß 1 each was diluted at 1;100 followed by the poly- horse radish peroxidase detection system with DAB as chormogen counterstained with Mayel ’s hematoxylin stain method and mounted And examined unde1' the biologic micro scope with the criteria of no epitheliaJ stain, weak 01' focal epithelial stain, moderate 01' focal intensive epithelial s tain. intense generalized epithelial staining for the epithelial, and connective tissue components in no1'mal salivary gland, and saivary g land tumors : pleomorphic adenoma‘ adenoid cystic carcinoma, adenoca1'cinoma, mucoepide1'moid ca1'cinoma on each On the integ1'in α 3 reaction, negative to minimal posit ive reaction was noted on the salivary gland twnors and nor mal subma ndibular gland tlssues On the integrin ß 1 reactions, intense 1'eaction is shown on the serous demilune and ductal cells , and partly on the serous acini in submandibula1' gland tlssues On the integrin ß 1 reactions to pleomorphic adenoma tissues, moderate reactions were noted on the ductal celJs and myoepithelial cells. On the integrin ß 1 reactions to adenoid cystic ca rci noma‘ adenocarcinoma, mucoepidermoid ca1'cinoma tissues, intense reactions were shown on the neo plastic cell s , This resuJt suggest that integrin a 3. integrin ß 1 could be a 1'ole inducing the tumorigenesis.
4,000원
3.
2007.10
구독 인증기관 무료, 개인회원 유료
Many researchers are interested in wound healing in the t reatment of burns, prevention of post surgical adhesions and cosmetic s urgery by excess collagen production and scar formatlOn Synthetic epidermal substi tutes with cultured epi thelial cells seem to be an attractive strategy since keratinocytes have been demonstrated to modulate fibroblast growth and collagen synthesis. Bioa bsorbable and biocompatible chitosan structurally mimics hyaluronic acid. Recently, a bio compatible synthesi zecl ch itosa n-PVP(polyvinyl pyrrolidone) hydrogels demonstrated in vitro biocompat ibi li ty for bio medical applications . However. there is no re port on this hydrogeJ"s ability to modulate human gingival fibroblast growth. The purpose of this study were to investigate different growth modulation between human gingival fibroblast and normal human oral keratinocyte by chitosan- PVP hydrogel, and to apply this biocompatible synthetic polymer to oral and maxillofacial wound healing. We have synthesized a hydrogel from chitosan-PVP and examined its effect on human gingival fibroblast growth modulation in vitro. Non-toxic and biocompatible hydrogel with human gingival fi broblasts and epithelial cells was tested by MTT assay. HGF showed a higher growth proliferation than that of NHOK after cell seeding. In MTT assay, 30% hydrogel leach out products showed a higher cellular viability in NHOK than that of any other products. In MTT assay, 30% hyclrogel leach out products showed relatively lower cellular viability of HGF ln growth profile, NHOK showed about 7 fo lcls higher than HGF after 1 day, while about 2 fo lds higher after 5 days. And also NHOK showed above about 70% cell ular via bility from 1 to 7 days. It suggested that Chitosan-PVP hydrogel would inhibit relatively the growth of HGF and s timulate the growth of NHOK_ This phenomenon may prove to be of use in wound management 0 1' oral and maxillofacial area as epitheli al substitutes.
4,000원
4.
2007.10
구독 인증기관 무료, 개인회원 유료
Laser is used to prevent the early dental caries in dental f ield and to apply for treatment of stomatitis and hyper sens it ivity , and laser mass Recently it is reported that laser i1'r adiation affect on soft tissue treatment and bone 1'emodelling after dental implantation. The purpose of this study was to examine laser irradia ti on effect on activity of normal human osteoblast on titanium plate in vitro by various laser wave length, and to observe morphologic change of NHost on LiLa nium plate and to a nalysis concentration of Ca"++ , IP and ALP NHost were cultured in DMEM containing 10% FBS, and observed by in verted microscope 1"or attatchment to the surface of titanium plate. Ca ++, I.P. , and a lkaline phosphat ase(ALP) concent ration in medium was calculated during 4 weeks, which was treated with Wilcoxon rank, Anova test and linear regression. The obtained results were as follows Morphologic changes showed rapid growth rate of NHost ++ at 3 days of laser lrradiatlOn ln spite of laser wave type, Ca" and P concentration was decreased at 2 weeks and was the hig hest at 3 weeks, but decreased at 4 weeks In spite of laser wave type, ALP concentration was decreased at 2 weeks but was increased at 3-4 weeks, From the aboving results, in spite of laser wave type, there were rapid growth rat e of NHost a nd no significant of Ca"++ , IP and P concen tr ation but these concentration showed predominant change than that of control
4,300원
5.
2007.10
구독 인증기관 무료, 개인회원 유료
Human gingival fibroblasts are necessary for oral homeostaslS These cells are fundamental in tissue healing and tissuc remodeling processes under a response to physiological actions such as mastication, Collagen and elastin, that are extracell ular glycoprotein of gingival fibroblast, are found in all animals, '1γpe 1 collagen is most dominant protein found in human gingival fibroblasts , Matrix metalloproteinase-1(MMP-1) has a role play in destruction of metabolism of extracellular matrix(ECM) and MMP-1 can destroy many ECMs as well as non-ECM molecules MMP-1’s local activation is conytolled by tissue inhibitor of metalloproteinase-1(TIMP-1) , Therefore, it is important to have a balance between in both s ituations MMPs and TIMPs of increased 0 1' decreased extracelluar matrix molecules, The purpose of trus study is to find out the effect of physical stimulus to human gingival fibroblast on mRNA, proteins of collagen 1, elastin, MMP-1, and TIMP-1 Healthy human gingival fibroblasts were separated and cultur때 in DMEM(Dulbeco’s Modified Eagle’s Medium) , When the sample reached to confluence state, it was separated with 0,25% t rypsin and 0‘ 53mM ethylendiaminetetraacetic aCld Separated cells were centrifuged in a cell culturing flask at 1000rpm for 30, 60, 120 mmutes Then it was forced by 35g/cm' continuously, The obtained results that expression of mRNA using histological study and Reverse Transcription Polymerase Chain Reaction (RT-PCR) , expression of protein using Enzyme-Linked Immunosorbent Assay(ELISA) for this study, At 30minutes after cen trifuging, there were s pindl e shaped gingival fibroblasts with long processes parallel to other cells in the control group , However the cell density was simil ar to compared group, At 60minutes after centrifuging, spindle shaped human gingival fibroblast with relatively long process, less densely packed, At 120minutes after centrifuging, cell processes were lengthened 2-3 times‘ and cell density was lower, At 30-60 minutes after centrifuging, it was increased by 1,3-1,7 times in expressoin of collagen 1 mRNA as compared with comparison group, However, there was no change in elastin, TIMP-l, and MMP-1, At 120 minutes after centrifuging, The revealed collagen 1 mRNA was increased 3 times as compared with comparison group, It was increased 2 times in elastin , 12 times in TIMP-1 as compared with comparison group, However, there was no change in MMP-l. At 30-60 minutes after centrifuging, it was increased by 1.1 times in revealing of protein revealing in collagen 1, TIMP-1 But there was no cha nge in elastin, MMP-1 At 120 minutes after centrifuging, it was increased by 1,2 times in revealing collagen 1 protein, 11 times in elastin, 12 times in TIMP-l, but there was no change in MMP-l. ln conclu s ion, it increased in revelation of collagen 1 ,elastin and TIMP-1 by continous stimulus in human gi ngival fibroblast, But there was no change in revelation of MMP-l Therefore, th is type of pressu re is one of the components for healing of gingiva l fibroblast
4,000원
6.
2007.10
구독 인증기관 무료, 개인회원 유료
A case of a distinctive variety of basalαd squamous cell carcinoma (B8CC) of the floor of mouth is described Histologic evaluation of the tumor showed lobules and aggregates of medium-sized basaloid cells with distinctive periph eral palisading and focal areas of comedo- necrosis. This appearance together with microcystic spaces simulated that of an adenoid cystic carcinoma. Accompanying epithelial dysplasia of the overlying mucosa was found. Immunohistochemistry of the tumor revealed diffuse strong expression for cytokeratin AE1/3, focally positive for Epithelial Membrane Antigen in the inner cells of tubular structures However, CEA, 8-100, smooth muscle actin, and vimentin were negative. The histologic differential diagnosis considered were adenoid cystic carcinoma and adenosquamous cell carcinoma Immunohistochemistry and awareness of this unusual pattern of B8CC will facilitate the correct diagnosis being reached.
3,000원
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