In the expansion of odontogenic cyst, degradation of extracellular matrix and resorp디on of bone are accompa띠ed ， but its mechanism is under debate. The degrees of inflammation and fibrosis were graded in 39 cases of odontogenic cysts. Cytoplasrnic expressions in various cell types according to them were analyzed using immunohistochemis띠， for MMPs and πMPs. In epithelial cells, MMP-2 expression was increased with marked fibrosis(p=O.018) and in stromal cells, MMP-2,-9 and TIMP-2 expressions were slightly higher in cases with mild inflammation and marked fibrosis. Tendencies of positive correlation were found between MMP-2,-9 and TIMP-2 expressions in epithelial cells and between MMP-l and MMP-9 expressions in stromal cells. These results suggest that MMP-l ,-2 ,-9 and TIMP-2 expression might be related to the expansion of odontogenic cysts, and the expression rate of them was different according to cell types and the degree of inflammation and fibrosis.
The purpose of this study was to investigate the initial bone tissue change and ,to repair on the Miniscrew and surrounding tissue under the various types orthodontic forces by use of niti closed spring through the polarizing φ>larizing microscopic fmdings. For this study, three young adult mongrel dogs(experimental group 1, 2, 3) were used, and 12 titanum miniscrew(a1, a2, a3, b1, b2, b3, c1, c2, c3, d1, d2, d3) were inserted into the palatal bone. The experimental design was consisted of equal opposite group(B), extrusion group(C), double same sided group(D). and group(A) was controlled. Group B, C, D miniscrews were loaded with 150gm of force immediately after placing to palatal side, and group B miniscrews were loaded with changed force of equal amount to buccal side after 10 없ys. and Group C was loaded extrusive force, group D was loaded double amount to palatal side at same time. The experimental animals in each group and control group were sacrified at one, three, and six weeks following implatation the miniscrew to make the samples for polarizing microscofic findings on palatal bone and miniscrew. All samples were examed and compared the 비stologic changes through the polarizing microscpe. The obtained results were as follows. 1. Under equal 01ψ。site force group, the histologic features of tissues around Miniscrew in experimental B group was no difference between the control group under equal opposite force group 2. Under extrusive force after lateral force group, it's bone density was decreased less than control groupunder extrusive force after lateral force group. πùs group was most p∞r bone densi마 ofall. 3. Under double same side group, inital feature was sirnlar to control group , but after 6weeks bone density was decreased along the Miniscrew surfaceunder double same side group. Based on these experimental results, in the prac디ce ， Miniscrew was usefull as skeletal anchorage in immediately load after implantation on alveolar bone under lateral force and opposite lateral force, but at applied extrusive force and double opposite side force were non- useful as skeletal anchorage with long-term treatrnent.
It is well known that glycoproteins, glycosaminoglycans and proteoglycans are of fllndamental importance to the processes of morphogenesis and cytodifferentiation dllring the teeth development. With HID-TCH-SP(High-iron diamine-thiocarbohydrazide-silver proteinate), slllfated glycosaminoglycans sllch as chondroitin slllfate and heparan slllfate have been localized at the 1I1trastructurallevel in a wide variety of tisslles. 까le pllrpose of this study were to characterize slllfated glycosaminoglycan profiles of hllman fetal tooth genns at 비trastructurallevel for the phase of morphogenesis and cytodifferentiation, and to detect the protein expression of sulfated glycosaminoglycan by immunoslot blot. Human tooth germs from the alveolar bone of twenty still born fetuses were f1xed in a mixture of 2% glutaraldehyde/ l% fonnaldehyde. The ultrathin sections were stained with HIDTCH- SP, and some sections were σ'eated with 0.05% solution of testicular hyaluronidase to identify the histochemical properties of the HID-TCH-SP stain deposits. For semiquantitative protein assay, immunoslot blot was done. The obtained results were as follows 1. HID-TCH-SP staining showed sulfated glycocongugated deposits in DEJ, peritublllar dentin, and mantle dentin matrix, enamel prism sheath, interrod area, and enamel matrix. 2. Heparan sulfate deposits in DEJ resisted to testicular hyalllronidase treatment prior to HID-TCH-SP staining 3. In immunoslot blot, chondroitin slllfate was detected higher in enamel and dentin extraα ， while heparan slllfate was relatively expressed in enamel and dentin extract, but rarely expressed in enamel or dentin extract. From the aboving results, it was suggested that chondroitin and heparan sulfate would play an important role in the formation of D티， while chondroitin sulfate would in the development of enamel prism sheath, enamel matrix, and mantle or peritllblllar dentin of human fetal t∞th germs.
Arrùno acid transpoπers play an important role in supplying nutrition to cells and for cell proliferation. System L is a major nutrient transport system responsible for the Na+-independent transport of large neutral amino acids including several essential amino acids. In malignant tumors, a system L transporter L-type amino acid transporter 1 (LATl) is upregulated to support tumor cell growth. In the present study, we have examined the expression and function of system L amino acid transporter in FaDu human pharyngeal squamous carcinoma cells. RT-PCR, real-time quantitative RT-PCR and westem blot analysis have revealed that the FaDu cells express LATl together with its associaω19 protem 4F2hc, whereas the FaDu cells do not express the other system L isoform L-type amino acid transporter 2 (LAT2). 까le uptake of L-(14Clleucine by FaDu cells is Na+-independent and almost completely inhibited by system L selective inhibitor 2-aminobicyclo-(2,2,1)-heptane-2- carboxylic acid (BCH). The profiles of the inhibition of L-[I4Cllellcine uptake by variolls amino acids in the FaDu cells are comparable with those for the LA T1 expressed in Xenopus 。()(찌es. π1e majority of L-[I4Clleucine uptake is, therefore, mediated by LAT1 in the FaDu cells. These results suggest that the transport of neutral amino acids including several essential amino acids in the FaDu human pharyngeal squamous carcinoma cells mediated by LAT1. In addition, specific inhibition of LAT1 by such agents as BCH in pharyngeal squamous cell carcinomas will be a new rationale for anti-cancer therapy.
Eugenol (4-allyl-2-methoxyphenol) is a phenol derivative and generally used in dental treatment. A few investigator reported that eugen이-induced C)πoto잉city by apopto디c pathway, but it is not yet well understood In the present study, to investigate the eugenol-induced cytoto잉city by apoptosis, we have examined the apoptotic molecules and pathway in primary human gingival fibroblast (HGF) and human salivary gland cells (HSG). To identify apoptotic cell death, 3-(4,5-dimethylthiazol-2-yl)-2 ,5-diphenyl tetrazolium bromide (MTT) reduction assay with or without N-acetylcysteine (NAC), and the morphological study by propidium iodide (pI) staining were screened. And to investigate the apoptotic pathway, reverse transcriptase-polymerase chain reaction (RT-PCR) for apoptotic molecules and caspase aαivity assay were performed. Both M1T reduction assay and an addition of NAC showed that eugenol act as a pro-oxidant led to cell death. With the morphological study, both cells showed apoptotic change by nuclear fragmentation and/or chromatin condensations. With the apoptotic machinery study, the Bax and Bcl-2 mRNA expression were not detected in HGF. But, for HSG, the increased expression of Bax with decreased of Bcl-2 was observed. And the expression of Apaf-l was not detected or nα significantly increased in HGF and HSG, respectively. With measure of caspase activity, there was no change of caspase activities in HGF. But, for HSG, there was decrease of caspase 9 activity and increased caspase 3 activity. We suggest eugenol-treated HGF underwent apoptosis independent of Bcl family and caspase. However, for eugenol-πeated HSG, apoptosis occurred via Bcl famiIy and caspase pathway.
A case was reponed in which an odontogenic cyst that appeared to be a dentigerous cyst associated with an impacted mandibular third molar was found histologically to demonstrate characteIistics of glanclu lar odontogenic cyst with para- and orthokeratinization. 까1ese histologic cliversities were interpreted as a reflection of the pluripotentiality of the epithelial remnants of the mandibular thircl molars or clentigerous cyst epithelium. It was conceivable that it would have the capacity of inclucing the fonnation of cysts with both squamous and glandular epithelium.