Urokinase-type plasminogen activator(uPA) bound to urokinase plasminogen activator receptor(uPAR) expression is strongly correlated with the metastatic potential of various tumors by enhancing ECM degradation through plasminogen and matrix metallopreotease activation. But expression of uPA/uPAR in human malignant salivary gland tumors has been rarely reported. The purpose of this study were to investigate mRNA expression and cytologic concentration of uPAR in SGT cell line compared to various cancer cell lines by RT-PCR and ELISA method, and to study migration and adhesion assay. These results would be to apply the pathogenesis and prognosis of malignant salivary gland tumors. All the cell lines(SGT, HN 4, SCC 25, and HeLA) were cultured under DMEM with 10% FBS at 37℃ in a 5% CO2 incubator. We studied a possible association between mRNA expression and cytosolic concentrations of uPAR in SGT cell line compared to various cancer cell lines using RT-PCR and an enzyme-linked immunoassay( ELISA) method. And also cell adhesion and migration assay were done in all the cell lines. In migration assay SGT cell line was about 2.5-4 folds higher than another cell lines. In adhesion assay SGT cell line was about 1.1-2 folds higher than another cell lines. uPAR cytosolic concentrations of SGT cell line was about 3.4-10 folds by ELISA, while mRNA expression was about 2.5-5 folds by RT-PCR. Oral Scc cell lines showed the lowest value. uPAR protein and mRNA expression were correlated with migration and adhesion assay. From the aboving results, high cytosolic concentrations and mRNA expression of uPAR were correlated with migration and adhesion assay. It suggested that this might be a specific marker for malignant potential of SGT cell line and would be contributed to pathogenesis and prognosis of human salivary gland adenocarcinoma
Recurrence-metastasis status of squamous cell carcinoma of tongue is a challenging oncologic problem. This study examined the expression of E-cadherin/β-catenin cell adhesion complex in squamous cell carcinoma of the tongue through an immunohistochemical study. Twenty samples from 15 patients with squamous cell carcinoma of the tongue, who were treated at the Department of Oral and Maxillofacial Surgery, consisted of primary or recurrent tumors along with matched metastatic lymph nodes were retrieved for immunohistochemical staining and grouped based on recurrence-metastasis status.Differences in stain localization were noted in E-cadherin, β–catenin and phospho β–catenin staining between different tumor groups based on the recurrence-metastasis status. The number of phospho β-catenin stain positive cells was found to have a significant role in survival. E-cadherin confirms its role as a powerful individual differentiation indicator and the role of β-catenin specially the phospho type elicts interest
Nodular fasciitis is defined by the World Health Organization as a benign proliferative fibroblastic lesion. It was first described in 1955 by Konwaler et al. and also known as “nodular pseudosarcomatous fasciitis” and “infiltrative fasciitis”, which reflect the lesion’s rapid growth, dense cellularity, alarming mitotoic activity and lack of circumscription. Here, we report a case of nodular fasciitis, which appeared as subcutaneous mass of right perizygoma area in 40-year-old Korean female. She had no history of trauma or cosmetic surgery related with the lesion. She disclosed that the mass grew rapidly for last 2 months. Computed tomography showed relatively well-defined spherical mass with 1.2 cm in diameter. Under local anesthesia the mass was resected by intraoral approach. The histological examination revealed a relatively poor-dermacated mass with high cellularity, which was composed with infiltrative spindle-shaped cells without accompanying inflammatory reactions. The tumor cells were partially positive for α-smooth muscle actin and β-catenin, but negative for cytokeratin AE1/3, S-100, desmin, and CD34. Therefore, the lesion was pathologically diagnosed as nodular fasciitis. Although nodular fasciitis is known to pseudosarcomatous benign reactive lesion, the clinician need to follow up the patient periodically. The reasons are as follows; Nodular fasciitis may accompany nuclear accumulation of β-catenin, which imply neoplastic process rather than reactive process of the lesion. Moreover, it has many histological similarities with inflammatory myofibroblastic tumor which have malignant transformation tendency. Further research for mesenchymal tumor could help our understanding for the disease entity of nodular fasciitis
Myositis ossificans traumatica(MOT) in head and neck area is a rare non-neoplastic, heterotopic bone formation within a muscle, arising after trauma or surgery. Although the several case reports have described the occurrence of MOT in other muscles of oro-facial musculature, MOT has never been reported in mentalis muscle. We demonstrate a case of MOT occurred in mentalis muscle of a 64 years old male. He recently complained of a movable mass with no pain. He had experienced a hitting trauma on his right anterior mandible about 25 years ago. Since then, the trauma area has been asymptomatic. The lesion showed severe muscular degeneration, resulted in a pseudocystic change without epithelial lining. The ectopic ossification was closely associated with the fibrous fascia of adjacent muscles. The lesion contained a lot of basophilic materials admixed with atrophying cells, which were supposed to be derived from muscle parenchyma injured previously. It was presumed that the basophilic materials induced callus-like ossification in the fibro-muscular lesion, also recruited macrophages and fibroblasts. However, the ossification processes in MOT were predominantly characterized by dystrophic calcification without osteoblastic differentiation of mesenchymal cells. Even more, there appeared some skeletal muscle bundles which were calcified dystrophically. Therefore, we suggest the present case of MOT might be initiated by dystrophic calcification in the degenerating muscle tissue
Both synovial cysts and synovial chondromatoses originate from synovial tissue, and only one case has been reported to occur simultaneously in a patient. Our patient presented with a swelling of the right preauricular area and pain on opening the mouth. Magnetic resonance images demonstrated two cystic structures in the superficial lobe of right parotid gland. Under the clinical impression of a cyst in the salivary gland, an enucleation was performed. However, the final diagnosis based on microscopic examination was a synovial cyst associated with the right temporomandibular joint and a synovial chondromatosis arising within the cystic wall. Awareness of this combined lesion will help make a diagnosis and establish a proper treatment plan.
A 41-year-old male patient complained of a whitish growing mass on the central area of palate. The exophytic keratinizing mass was recently grown and showed rough surface localized at palatal mucosa. He has heavily smoked for more than 25 years. The lesion was clearly demarcated as an out-growing verrucous whitish epithelial lesion with erosive mucosa in the periphery and also continuous with the extensive hyperkeratosis in whole hard palatal mucosa. In the histological examination there appeared a plaque type verrucous hyperplasia in the absence of papillomatous growth. In the series of immunostains including PCNA, p53, β-catenin, APC, E-cadherin, BCL-2, MMP-1 and MMP-3 showed no features of infiltrative growth and malignant transformation. Therefore, the present case was diagnosed oral verrucous hyperplasia(OVH) different from squamous cell papilloma, verrucous leukoplakia, verrucous carcinoma, and oral squamous cell carcinoma.