Background and Purpose: Antimicrobial photodynamic therapy using Methylene blue (MB-PDT) has been proposed as an adjunctive to scaling and root planing (SRP) to provide preferable results for the treatment of periodontitis. The multi-factor mechanism of aPDT action correlates with various influencing components such as the photosensitizer and the light delivery system. The paper aims to review the recorded parameters of MB-PDT from clinical trials of periodontitis which may serve to improve the treatment of periodontal diseases. Materials and Methods: PubMed search engine was used to identify human clinical trials of PDT in dentistry. After applying specific keywords, additional filters, exclusion criteria, the initial number of 17378 was reduced to 12. Results: More than half of the articles of SRP + MB-PDT presented better results [pocket depth (PD) reduction, clinical attachment level (CAL) gain, etc.] compared to SRP alone in the treatment of periodontitis. Conclusions: While more clinical evidence is needed, recent studies demonstrate that MB-PDT combined with SRP show a greater potential as a treatment of periodontal diseases in comparison to SRP alone.
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck cancer and is associated with high recurrence, poor treatment, and low survival rates. Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that regulates the response to hypoxia, a major factor in the tumor microenvironment that affects tumor development and progression in various cancer types. However, microRNA (miRNA) sequence analysis revealed that only a few miRNAs targeting HIF-1α had been discovered. In the present study, we investigated HIF-1α expression in OSCC and the effect of HIF-1α-targeting miRNAs on the progression and metastatic potential of OSCC. We analyzed public databases to explore which miRNAs target HIF-1α expression. In addition, the expression of proteins involved in the cell cycle, proliferation, and apoptosis in HSC-2 cells was analyzed after miRNA-126 mimic treatment. Furthermore, to investigate the effect of miRNA-126 on the proliferation and invasion ability of OSCC cells, 5-ethynyl-2′-deoxyuridine and Transwell assays were performed. The activities of MMP-2 and MMP-9 were evaluated via gelatin zymography. Our results showed that miRNA-126, which targets HIF-1α, enhances OSCC cell proliferation by regulating the cell cycle and reinforces the cell mobility of OSCC via HIF-1α expression. These findings suggest that miRNA-126 may be a novel marker for OSCC treatment and the development of new tools for patients with OSCC.
According to the classification of World Health Organization, primary adenocarcinomas of the sinonasal tract can be initially classified as salivary and non-salivary types. The latter are further divided into intestinal and non-intestinal types. Sinonasal intestinal-type adenocarcinoma(ITAC) is rare adenocarcinoma subtype, which is closely occupational exposure to hardwood dusts, leather. In this study, we present a case of ITAC in a 68-year-old man. We successfully treated with wide excision and soft tissue reconstruction with free anterolateral thigh flap.